Kinetics Approach of Biodegradation of Petroleum Contaminated Soil by using Indigenous Isolated Bacteria

Yudono, Bambang; Said, Muhammad Irfan; Sabaruddin, .; Napoleon, Adipati; Fanani, Zaenal

doi:10.5400/jts.2011.16.1.33

Cited by 18 publications

(4 citation statements)

References 2 publications

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“…Considering the degradation efficiencies of bacterial strains, the highest rate constant was shown with A1 strain (2.533 day −1 , t 1/2 = 0.273) followed by A2 (1.648 day −1 , t 1/2 = 0.420), A3 (1.272 day −1 , t 1/2 = 0.545) A4 (1.887 day −1 , t 1/2 = 0.367) in a decreasing order. The results are also consistent with studies in the literature [62,63].…”

Section: Bio-removal Of Petroleumsupporting

confidence: 93%

Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field

Özyürek

Soyuer

2023

Hacettepe Journal of Biology and Chemistry

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This study aimed to enhance the removal of petroleum with potent biosurfactant producing bacterial strains (Bacillius subtilis CC9, Enterobacter asburiae WMB1, Klebsiella oxytoca Rizhao 536-1, and Acinetobacter pittii PgBE252) isolated from an oil field. The biosurfactant production was determined by different screening methods such as hemolytic activity, drop-collapse, oil spreading, BATH, emulsification index and surface tension. While the presence of biosurfactant was detected with individual bacterial strain, B. subtilis CC9 strain was found to be quite effective on petroleum degradation (92%) with a high yield of biosurfactant production (0.95 ±0.05 g L-1). The degradation rate constant and half-life period of B. subtilis CC9 strain were calculated as K = 2.533 day−1, t1/2 = 0.273. It has been clearly shown with GC/MS analysis that B. subtilis CC9 strain was effective on the removal of long chain n-alkanes (C31 - C35) as well as short and medium chains. The FT-IR analysis also approved the presence of aliphatic and peptide moieties of biosurfactant extracted from B. subtilis CC9 strain. It is remarkably pointed out with this study that high yield of biosurfactant producing B. subtilis CC9 strain can be used in advanced petroleum bioremediation studies as an alternative to chemical surfactants.

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Section: Bio-removal Of Petroleumsupporting

confidence: 93%

Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field

Özyürek

Soyuer

2023

Hacettepe Journal of Biology and Chemistry

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“…In a similar study, the reaction rate constants of Pseudomonas pseudoalcaligenes, Bacillus megaterium and Xanthobacter autotrophicus were 0.0189, 0.0324 and 0.0324 day −1 , respectively. According to the reaction rates, Xanthobacter autotrophicus was the most effective bacteria in the degradation of petroleum oil sludge [42]. Considering the results in the literature, it was clearly stated that the halflife period was low with a high degradation rate constant in consortium 3, which had the highest biodegradation rate.…”

Section: Evaluation Of Growth Intensities and Petroleum Biodegradatiomentioning

confidence: 97%

Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

Özyürek

Bilkay

2020

SN Appl. Sci.

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The objectives of this study were to determine and compare the petroleum biodegradation efficiencies of three different bacterial consortia adapted to oil field. For this purpose, petroleum-contaminated waste mud sample was enriched in Bushnell Haas medium with 1% petroleum. Six different bacterial strains (four Gram-positive and two Gram-negative) were isolated from the enriched sample, and bacterial strains were identified with phenotypic and genotypic analyses. Each bacterial strain was evaluated in terms of biodegradation capacities by colorimetric analysis with the DCPIP redox indicator. Petroleum biodegradation efficiencies of three different consortia were investigated by gravimetric and GC-MS analyses. Consortium 1, consisting of Bacillus subtilis, Leuconostoc mesenteroides, Bacillus halotolerans, Enterococcus casseliflavus; consortium 2, consisting of Klebsiella pneumoniae and Pannonibacter phragmitetus; consortium 3, consisting of Bacillus subtilis, Leuconostoc mesenteroides, Bacillus halotolerans, Enterococcus casseliflavus, Klebsiella pneumoniae and Pannonibacter phragmitetus, were used in petroleum biodegradation. According to the results of gravimetric analysis, biodegradation of petroleum was 48%, 77% and 82% for consortium 1, consortium 2 and consortium 3, respectively. The results of GC-MS analysis revealed that removal of long-chain n-alkanes (C 23-C 32) was in the range of 35-58% by consortium 1; 56-67% by consortium 2; and 63-74% by consortium 3. It was emphasized that consortium 2 was more effective in biodegradation of petroleum than consortium 1. This study clearly demonstrated that consortium 3 is most effective in biodegradation of petroleum. This is assumed to be due to the synergistic effect between Gram-positive and Gram-negative bacterial strains. Furthermore, consortium 3 has been found to be very successful despite the shorter incubation period compared to other consortia in the literature. In this context, it is believed that the use of consortium 3 will contribute to the bioremediation of petroleum on a large scale.

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“…Attenuation of petroleum hydrocarbons in terms of biodegradation has been shown to follow a first order reaction [21][22]. The plots in Fig.…”

Section: Predicted Time For Complete Biodegradationmentioning

confidence: 99%

Effect of Aerating Duration on Hydrocarbon Biodegradation in a Simulated Crude-Oil Polluted Aquatic Environment Undergoing Bioremediation

Ewoh

Peekate

2021

SAJRM

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The aim of this research work was to determine the aerating duration that would be effective in enhancing hydrocarbon biodegradation rate during bioremediation of crude-oil polluted river. Sediment and river-water were placed in four glass troughs labeled CT (control), A, B, and C. The setups were polluted with crude-oil, and allowed undisturbed for 2 weeks. Subsequently, accessible crude-oil on the surface was removed; bacteria and nutrients were then added. Air was bubbled for 3 hours into setups A, B, and C, at daily, 3 days, and 7 days interval respectively. Aeration was not applied to setup CT. On day 1, 7, 14, and 21, hydrocarbon concentration was determined; populations of total heterotrophic bacteria (THB) and hydrocarbon-utilizing bacteria (HUB) were also determined. The time it will take for hydrocarbons in the setups to biodegraded “completely” was calculated using first-order reaction equation. The results obtained showed that 71.43, 86.39, 83.17, and 15.42 % hydrocarbon degradation were obtained in setup A, B, C, and CT respectively. The time it will take for hydrocarbons in the setups to biodegrade “completely” were 129, 89, 101, and 1079 days for A, B, C, and CT respectively. There was slight reduction in population of HUB in setup CT, fairly stable population in setup A, and increase in population of HUB in setups B and C. It is concluded that aerating crude-oil polluted aquatic environment for 3 hours at 3 days interval will be more effective in enhancing hydrocarbon biodegradation rate during bioremediation.

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Kinetics Approach of Biodegradation of Petroleum Contaminated Soil by using Indigenous Isolated Bacteria

Cited by 18 publications

References 2 publications

Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field

Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field

Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

Effect of Aerating Duration on Hydrocarbon Biodegradation in a Simulated Crude-Oil Polluted Aquatic Environment Undergoing Bioremediation

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