Kinetics and stoichiometry of growth of plant cell cultures of <i>Catharanthus roseus</i> and <i>Nicotiana tabacum</i> in batch and continuous fermentors

Gulik, Walter M. van; Hoopen, H. J. G. ten; Heijnen, Joseph J.

doi:10.1002/bit.260400802

Cited by 57 publications

(25 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While literature data for the product formation model parameters are not readily available, the growth model parameters reported here are in good agreement with the literature data. The values for m max (0.323/day) and Y X|S (0.474 g/g) found in this study are very similar to the values reported by Van Gulik et al (1992) for Nicotiana tabacum cell suspension culture (m max ¼ 0.3264/day and Y X|S ¼ 0.57 g/g). The validity of the dynamic state model was further scrutinized using data from another three independently conducted reactor runs (runs #2$4; each has a different initial glucose concentration and slightly different initial cell density) using the model parameter values shown in Table I.…”

Section: Development Of a Dynamic Process Modelsupporting

confidence: 89%

Observer‐based online compensation of inner filter effect in monitoring fluorescence of GFP‐expressing plant cell cultures

Liu

et al. 2005

Biotech & Bioengineering

View full text Add to dashboard Cite

The green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria is a very useful reporter for real-time bioprocess sensing. GFP culture fluorescence is a composite signal that can be influenced by factors such as culture autofluorescence, inner filter effect (IFE), and photobleaching. These factors complicate accurate estimation of GFP concentrations from the culture fluorescence. IFE is especially problematic when using GFP in monitoring transgenic plant cell suspension cultures, due to the aggregated nature of the cells and the high biomass concentration in these culture systems. Reported approaches for online compensation of IFE in monitoring culture NADH fluorescence or bioluminescence require online measurement of biomass density or culture turbidity/optical density, in addition to fluorescence/bioluminescence measurement. In this study, culture GFP fluorescence was used successfully to estimate GFP concentration and other important states in bioreactor culture of transgenic tobacco cells, while the influences of IFE and culture autofluorescence were rectified without the need for an additional biomass sensor. This was achieved by setting up a novel model-based state observer. First, we developed an improved model for a backscatter fluorescence probe that takes into account the influence of IFE and autofluorescence on reporting culture GFP concentration from online fluorescence. The state observer was then established using the extended Kalman filter (EKF), based on the fluorescence probe model, a dynamic state model of the plant cell bioreactor, and online GFP fluorescence measurement. Several versions of the observer were introduced to address practical requirements associated with monitoring GFP fluorescence of plant cell cultures. The proposed approach offers an effective means for online compensation of IFE to enable quantitative interpretation of the culture fluorescence signals for accurate reporting of GFP or GFP-fusion protein expression.

show abstract

Section: Development Of a Dynamic Process Modelsupporting

confidence: 89%

Observer‐based online compensation of inner filter effect in monitoring fluorescence of GFP‐expressing plant cell cultures

Liu

et al. 2005

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Up to this point we have assumed that sugar is converted only to biomass and CO 2 . Plant cell cultures are, however, capable of producing extracellular polysaccharides (van Gulik et al, 1992). Furthermore, extracellular proteins and the products of cell lysis also accumulate in the medium, and these are considered byproducts in hairy root cultures (Kwok and Doran, 1995).…”

Section: Carbon Balancementioning

confidence: 99%

Growth dynamics of Artemisia annua hairy roots in three culture systems

Kim

Weathers

Wyslouzil

2003

Biotech & Bioengineering

View full text Add to dashboard Cite

The transient growth of Artemisia annua hairy roots was compared for cultures grown in shake flasks and in bubble column and mist reactors. Instantaneous growth rates were obtained by numerically differentiating the transient biomass measurements. Specific sugar consumption rates showed good agreement with literature values. From the growth rate and sugar consumption rate, the specific yield and maintenance coefficient for sugar were determined for all three culture systems. These values were statistically indistinguishable for roots grown in shake flasks and bubble columns. In contrast, the values for roots grown in bubble columns and mist reactors were statistically different, suggesting that sugar utilization by roots grown in these two systems may be different. By measuring respiration rates in the bubble column reactor we also determined the actual biomass yield and maintenance coefficient for O(2) and CO(2). Together with an elemental analysis of the roots, this allowed us to obtain a reasonable carbon balance.

show abstract

“…Microalgal cells can trap light as the energy source and assimilate CO 2 as the carbon source. It is important to note that the organic substrates can also be utilized as the carbon and energy sources by some microalgae [38,39,40]. Changes in the proportions of individual photosynthetic pigments may occur in response to variation in the intensity of light and a species in which a particular type of pigmentation is determined genetically may become adapted to an environment [40].…”

Section: Discussionmentioning

confidence: 99%

Improved Protocol for the Preparation of Axenic Culture and Adaptation to Heterotrophic Cultivation

Azma¹,

Mohamad²,

Rahim³

et al. 2010

TOBIOTJ

View full text Add to dashboard Cite

Abstract:The effectiveness of various physical and chemical methods for the removal of contaminants from the microalgae, Tetraselmis suecica, culture was investigated. The information obtained was used as the basis for the development of improved protocol for the preparation of axenic culture to be adapted to heterotrophic cultivation. Repeated centrifugation and rinsing effectively removed the free bacterial contaminants from the microalgae culture while sonication helped to loosen up the tightly attached bacterial contaminants on the microalgae cells. Removal of bacterial spores was accomplished using a mixture of two antibiotics, 5 mg/mL vancomycine and 10 mg/mL neomycine. Walne medium formulation with natural seawater was preferred for the enhancement of growth of T. suecica. Adaptation of growth from photoautotrophic to heterotrophic conditions was achieved by the repeated cultivation of photoautotrophic culture with sequential reduction in illumination time, and finally the culture was inoculated into the medium containing 10 g/L glucose, incubated in total darkness to obtain heterotrophic cells. Changes in the morphology and composition of T. suecica cells during the adaptation from photoautotrophic to heterotrophic condition, as examined under Transmission Electron Microscope, were also reported.

show abstract

Kinetics and stoichiometry of growth of plant cell cultures of Catharanthus roseus and Nicotiana tabacum in batch and continuous fermentors

Cited by 57 publications

References 48 publications

Observer‐based online compensation of inner filter effect in monitoring fluorescence of GFP‐expressing plant cell cultures

Observer‐based online compensation of inner filter effect in monitoring fluorescence of GFP‐expressing plant cell cultures

Growth dynamics of Artemisia annua hairy roots in three culture systems

Improved Protocol for the Preparation of Axenic Culture and Adaptation to Heterotrophic Cultivation

Contact Info

Product

Resources

About