Kinetics and Mechanism of Degradation of Ampicillin in Solution

Hou, Joseph P.; Poole, John W.

doi:10.1002/jps.2600580412

Cited by 93 publications

(37 citation statements)

References 19 publications

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“…This suggests that something else is occurring to the ampicillin S-oxide that changes the structure, but has the same exact mass. Investigations into the literature revealed the presence of a fluorescent compound as a result of ampicillin degradation and this was attributed to the formation of ampicillin diketopiperazine [56]. At this length of a treatment time, the solutions turned yellow in color.…”

Section: Mechanism Of Plasma Degradationmentioning

confidence: 99%

Mechanism of Ampicillin Degradation by Non-Thermal Plasma Treatment with FE-DBD

Smith

Adams

2017

Plasma

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This research focused on determining the effectiveness of non-thermal atmospheric pressure plasma as an alternative to advanced oxidation processes (AOP) for antibiotic removal in solution. For this study, 20 mM (6.988 g/L) solutions of ampicillin were treated with a floating electrode dielectric barrier discharge (FE-DBD) plasma for varying treatment times. The treated solutions were analyzed primarily using mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). The preliminary product formed was Ampicillin Sulfoxide, however, many more species are formed as plasma treatment time is increased. Ampicillin was completely eliminated after five minutes of air-plasma treatment. The primary mechanism of ampicillin degradation by plasma treatment is investigated in this study.

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Section: Mechanism Of Plasma Degradationmentioning

confidence: 99%

Mechanism of Ampicillin Degradation by Non-Thermal Plasma Treatment with FE-DBD

Smith

Adams

2017

Plasma

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“…A total of 88 male Wistar (Morini) rats aged 4 months, weighing 357 + 11 g, were used; all animals were housed under standard feeding and environmental conditions. The animals fasted for 18 h before treatment, were subdivided into 11 lots of 8, and then were treated orally or intraperitoneally with the antibiotics listed in Table 1 (3), with minor modifications, using the bacterial strains specified in Table 1 for the inoculum.…”

Section: Methodsmentioning

confidence: 99%

Stability of Frozen Rat Plasma Containing Different Antibiotics

Berti¹,

Maccari²

1975

Antimicrob Agents Chemother

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The antibiotic activity was determined at different intervals of time on plasma samples, taken from rats treated with a certain number of commonly used antibiotics, and kept at -20 C up to 8 weeks. The results of the microbiological assays demonstrate that the stability of the antibiotics in the frozen plasma decreases in the following order: oxytetracycline > cephalexin, streptomycin, erythromycin > demeclocycline > ampicillin, amoxycillin > penicillin G, cephaloridine, rolitetracycline, and tetracycline.

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“…Several studies have been reported on the stability of ampicillin in solution (5,8,11,12). It appears that the stability depends upon solvent, pH, temperature, and ampicillin concentration.…”

Section: Methodsmentioning

confidence: 99%

Rapid, Precise, Turbidometric Assay for Low Levels of Ampicillin in Serum After Single-Dose Oral Administration

Whyatt

Dann

Slywka

et al. 1974

Antimicrob Agents Chemother

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To date, procedures utilizing cup-plate microbiological assay (4, 19) and fluorometry (15) have been the only non-isotopic methods available to quantitatively measure ampicillin at the low concentrations found in urine and serum after therapeutic doses of the drug. However, ampicillin metabolites appear to interfere with determination of ampicillin by this fluorometric procedure (15). Problems inherent with cupplate microbiological assay systems include a 10 to 20% variability in precision, long incubation periods, the somewhat complicated procedures for calculating and plotting data, and the need for sample dilution to narrow ranges of concentration required for zonal inhibition. This latter problem is magnified when the drug is being assayed in samples of biological fluids.Because of these limitations, we have developed a turbidometric assay for ampicillin in serum. With this assay, the entire range of serum concentrations observed after a single oral dose of 250 mg can be determined directly, without dilution and with a high degree of precision. MATERIALS AND METHODSGeneral procedures. Several organisms were tested for susceptibility. The

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Kinetics and Mechanism of Degradation of Ampicillin in Solution

Cited by 93 publications

References 19 publications

Mechanism of Ampicillin Degradation by Non-Thermal Plasma Treatment with FE-DBD

Mechanism of Ampicillin Degradation by Non-Thermal Plasma Treatment with FE-DBD

Stability of Frozen Rat Plasma Containing Different Antibiotics

Rapid, Precise, Turbidometric Assay for Low Levels of Ampicillin in Serum After Single-Dose Oral Administration

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