Kinetics and isoforms of serum glutamate dehydrogenase in alcoholics

Kravos, Matej; Malešič, Ivan

doi:10.1093/alcalc/agn010

Cited by 16 publications

(14 citation statements)

References 24 publications

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“…We calculated the percentage of GLDH inactivation, with the corresponding thermolabile isoenzyme. We took the 20% barrier of thermo stable as the lowest reliable percentage [4] of GLDH inactivation; this reflects the findings of Grossman, as he was able to inactivate approximately 17% of soluble (thermostabile) and approximately 49% of particulate (thermo labile) GLDH [38].…”

Section: Methodsmentioning

confidence: 75%

“…A statistically significant correlation was also found between total and inactivated GLDH in the cerebrospinal fluid and serum in patients with neurological diseases. The GLDH activity in cerebrospinal fluid is probably more decreased, because it is mitochondrial rather than GLDH from the rough endoplasmic reticulum [4] [5] [42] [43].…”

Section: Resultsmentioning

confidence: 99%

“…GLDH has the key role of acting as an interface between carbohydrates and amino acids in the vicinity of the citric acid cycle and urea cycle in the metabolism of a cell. Its function is to catalyse reversible deamination of glutamate into α-ketoglutarate and ammonium ion [2] [3] [4]. There are two forms: thermostable and thermolabile [1] [5] [6].…”

Section: Introductionmentioning

confidence: 99%

“…There are two forms: thermostable and thermolabile [1] [5] [6]. With thermal inactivation of serum, GLDH was found that almost one third of GLDH serum originated from the rough endoplasmic reticulum and the rest from mitochondria [4].…”

Section: Introductionmentioning

confidence: 99%

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The Role of Glutamate Dehydrogenase Activity in Development of Neurodegenerative Disorders

Kravos¹,

Malešič²

2017

WJNS

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The specific role of Glutamate dehydrogenase (GLDH) in the brain is not yet clear, but it is an important enzyme in protein degradation as well as a metabolism regulator of glutamate as a neurotransmitter. The enzyme probably provides crucial protection for postsynaptic membranes against the neurotoxic effects of glutamate neurotransmitters. In men, GLDH activity declines almost evenly through the ages; in women, it declines faster in the first five decades. In the years of menopause, GLDH activity declines slower. The diminished GLDH activities in leukocytes and in the brain vary considerably, but they are parallel with the progress of neurodegenerative diseases. The GLDH activity is partly deficient in the brain, particularly in the leukocytes of patients with heterogeneous neurological disorders and degeneration of multiple neuronal systems. We found a statistically significant difference of GLDH activity in the cerebrospinal fluid in patients with neurological diseases and unexpected in patients with degenerative and inflammatory disorders. The decrease in GLDH activity in the cerebrospinal fluid of patients with neurodegenerative disorders may be one of the reasons for the neuro-excitotoxic glutamate effect. Defining the GLDH activity in leukocytes is at the moment the sole experimental method. The second one could be the measurement in cerebrospinal fluid. The results suggest a possibility to regulate glutamate level in human brain through activation of GLDH.

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Section: Methodsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Role of Glutamate Dehydrogenase Activity in Development of Neurodegenerative Disorders

Kravos¹,

Malešič²

2017

WJNS

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“…Elevated GGT in conjunction with alkaline phosphatase usually points to hepatobilliary injury [5][6][7]. Serum GGT activity has commonly been interpreted as a marker of alcohol consumption or liver disease [8].…”

Section: Introductionmentioning

confidence: 99%

Repeat Whole Blood Donation Correlates Significantly with Reductions in BMI and Lipid Profiles and Increased Gamma Glutamic Transferase (GGT) Activity among Nigerian Blood Donors

Adias¹

2012

OJBD

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Background: The effect of repeated blood donation on some biochemical values of healthy adult male blood donors in Calabar, Nigeria was studied. Methods: One hundred and fifty three (153) healthy repeat blood donors and 90 first time blood donors constituted the study population. Samples were analyzed using colorimetric procedures. Results: The BMI values of 24.4 ± 2.4 kg/m 2 in the first time donors was significantly higher than the 21.7 ± 1.7 kg/m 2 obtained in repeat donors (P < 0.001). Among all the biochemical parameters, total cholesterol showed a significant change of 2.55 ± 0.74 mmol/l after repeated donations as compared to 3.45 ± 1.47 mmol/l in the first time donors (P < 0.005). Gamma glutamyl transferase (GGT) was significantly higher in repeat donors (70.5 ± 13.5 µl) than the first time donors (62.5 ± 13.3 µl) (P < 0.05). An LDL value of 1.02 ± 0.8 mmol/l in repeat donors was significantly lower than 1.69 ± 1.0 mmol/l in first time donors (P < 0.05). A VLDL value of 0.32 ± 0.2 mmol/l in repeat donors was equally lower than 0.44 ± 0.2 mmol/l obtained in the first time donors (P < 0.05). GGT showed positive correlation with lactate dehydrogenase (LDH) and low density lipoproteins (LDL) at P < 0.05 and P < 0.01 respectively among the repeat blood donors. Triglycerides showed a positive correlation with very low density lipoprotein (VLDL) among repeat donors at P < 0.05 significant level. Pearson correlation analysis also indicates that a significant positive relationship exists between GGT and low density lipoprotein (r = 0.891, P < 0.001). The regression analysis defined the relationship as linear (y = 0.0578x + 36.87; r 2 = 0.7934, P < 0.05). Conclusion: A reduction in the values of some lipid profiles and high GGT activity is associated with repeated blood donations in this study population. Repeated blood donation may play a significant role in reducing the incidence of heart disease.

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Biodegradable β‐Tri‐Calciumphosphate/hydroxyethyl methacrylate enhanced three component bone adhesive demonstrates biocompatibility without evidence of systemic toxicity in a rabbit model

et al. 2009

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Bone gluing is an attractive surgical technique; however, its use in patients is hampered by a variety of side effects. Therefore, it was the aim of this ethically approved study to evaluate a novel biodegradable beta-Tri-Calciumphosphate (beta-TCP, Cerasorb)-enhanced bone adhesive regarding its toxicity and biocompatibility in a rabbit model. Fifty healthy New Zealand White rabbits were assigned in the study (n = 21) and sham-operated control group (n = 29). In the study group, a cylindrical part (4.6 x 10.0 mm) of the proximal tibia and distal femur was removed, reimplanted, and bone adhesive was applied. Blinded physical examination and sampling for hematology, clinical chemistry, and acute phase proteins (haptoglobin, C-reactive protein (CRP)) was performed before surgery and after 12, 24, 48, 72, 240, and 504 h. Significant findings of the physical examination included a slightly higher grading of warmth (p = 0.0019) and pain (p < 0.0001) of the wound 240 h after surgery in the study group. No significant differences between albumin, haptoglobin, CRP, and urea concentrations in both groups were evident, whereas the study group demonstrated significantly lower leukocyte count, total protein, and globulin concentrations (p < 0.0001). As expected, both groups showed a marked transient increase in muscle enzymes (Creatine Kinase and Aspartate Aminotransferase) following the surgery (p < 0.0001). Twelve to 24 h after surgery, a significant decrease in ionized calcium from 1.38 +/- 0.12 mmol/L to 1.06 +/- 0.13 mmol/L was noted in the study group (p < 0.0001). The results clearly indicated that the novel beta-TCP-enhanced bone adhesive showed good biocompatibility without significant evidence of acute or subacute local or systemic toxicity.

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Kinetics and isoforms of serum glutamate dehydrogenase in alcoholics

Cited by 16 publications

References 24 publications

The Role of Glutamate Dehydrogenase Activity in Development of Neurodegenerative Disorders

The Role of Glutamate Dehydrogenase Activity in Development of Neurodegenerative Disorders

Repeat Whole Blood Donation Correlates Significantly with Reductions in BMI and Lipid Profiles and Increased Gamma Glutamic Transferase (GGT) Activity among Nigerian Blood Donors

Biodegradable β‐Tri‐Calciumphosphate/hydroxyethyl methacrylate enhanced three component bone adhesive demonstrates biocompatibility without evidence of systemic toxicity in a rabbit model

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