Kinetics and intimate mechanism of protein crystal nucleation

Nanev, Christo N.

doi:10.1016/j.pcrysgrow.2013.09.001

Cited by 21 publications

(20 citation statements)

References 94 publications

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“…These observations clearly indicate that the amorphous solid particles act merely as a heterogeneous substrate that enhances the nucleation event, proceeding according to CNT. (In other words, the assumption that protein crystal nucleates heterogeneously on foreign particles of biological origin [74] is confirmed by these experiments). All this marks a significant departure from the initial formulation of the TSN [75].…”

Section: Evoking Nucleation; Classical Nucleation Theory (Cnt) Vs Musupporting

confidence: 64%

Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

Nanev

2017

Crystals

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Three-dimensional protein molecule structures are essential for acquiring a deeper insight of the human genome, and for developing novel protein-based pharmaceuticals. X-ray diffraction studies of such structures require well-diffracting protein crystals. A set of external physical factors may promote and direct protein crystallization so that crystals obtained are useful for X-ray studies. Application of electric fields aids control over protein crystal size and diffraction quality. Protein crystal nucleation and growth in the presence of electric fields are reviewed. A notion of mesoscopic level of impact on the protein crystallization exercised by an electric field is also considered.Keywords: protein crystallization; classical and two-step nucleation mechanisms; impact of electric fields on the protein crystallization; external and internal electric fields; number density; size and quality of protein crystals

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Section: Evoking Nucleation; Classical Nucleation Theory (Cnt) Vs Musupporting

confidence: 64%

Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

Nanev

2017

Crystals

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“…The consequence of this fact is that the attachment frequency f* of protein molecule is extremely low compared to small molecule with isotropic property in its surface. Although the touch frequency of two molecules in protein solutions is almost same as the one of small molecule when the thermodynamic conditions are conserved, the highly anisotropic distribution along the surface causes the majority of such touches between protein molecules fail to create a bond in order to generate a nucleus [50]. Filled squared symbols are designated for the lysozyme solution 3.5% NaCl with 50 mg/ml; cross stands for 7.0% NaCl solutions without protein; empty triangles represent for 50 mg/ml lysozyme with 7.0% NaCl.…”

Section: Resultsmentioning

confidence: 99%

The influence of low frequency of external electric field on nucleation enhancement of hen egg-white lysozyme (HEWL)

Pan

Zhang

et al. 2015

Journal of Crystal Growth

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“…Energy barriers of crystal nucleation and numbers of molecules constituting the critical nuclei were calculated from the stationary nucleation rates measured. (For more results see . )…”

Section: Experimental Study On Protein Crystal Nucleationmentioning

confidence: 98%

Recent experimental and theoretical studies on protein crystallization

Nanev

2016

Crystal Research and Technology

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Crystals are required to determine three-dimensional structures of proteins and other bio-molecules via X-ray diffraction. This is the prime cause for the keen interest in protein crystallization. Recently published experimental and theoretical studies on protein crystal nucleation and growth are reviewed, and the prospects of protein-based crystalline pharmaceuticals mass-production are considered. A new insight into protein crystal nucleation is presented from a physical perspective. It refers to modeling nuclei number density as a definite (curved) surface where each point has two coordinates, supersaturation and time. In doing so, theoretical S-shaped dependences of nuclei number densities on both supersaturation and time are found. These dependences are confirmed experimentally by plotting insulin crystal nucleation data. Growth of protein crystals of narrow size distribution is monitored to establish crystal polydispersity cause. Ostwald ripening is considered as a postgrowth stage of the crystallization process.

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Kinetics and intimate mechanism of protein crystal nucleation

Cited by 21 publications

References 94 publications

Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

The influence of low frequency of external electric field on nucleation enhancement of hen egg-white lysozyme (HEWL)

Recent experimental and theoretical studies on protein crystallization

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