Kinetics and Histopathology of the Cacao-Ceratocystis cacaofunesta Interaction

Santos, Rogério Mercês Ferreira; Silva, Stela Dalva Vieira Midlej; Sena, K.F.; Micheli, Fabienne; Gramacho, Karina Peres

doi:10.1007/s12042-012-9115-8

Cited by 13 publications

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“…A histopathological study of the interaction between C. cacaofunesta and cacao showed plant responses to C. cacaofunesta infection in both resistant and susceptible plants [ 15 ]. Plant responses included the discoloration of the primary walls of infected xylem vessels and the surrounding parenchyma cells; the mobilization of polyphenols in parenchyma cells, the translocation and accumulation of starch in the xylem and the production of gums and tyloses [ 15 ]. The main differences between genotypes were the intensities and kinetics of the appearance of those responses being more pronounced in the susceptible varieties [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…On susceptible hosts, the fungus infects xylem parenchyma cells in a radial direction, from where generated hyphae reach and invade the xylem vessels [ 14 ]. C. cacaofunesta produces atypical smaller conidia which can pass through the cell wall pits and are probably involved in the rapid and massive plant colonization by the fungus [ 15 ]. As a necrotrophic fungus , C. cacaofunesta causes plant cell death during host colonization [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…Plant-fungal interaction studies have reported the formation of polysaccharide gels, tyloses, and phenolic compounds in the vascular vessels of plants infected by Ceratocystis species [ 15 , 21 ]. CWC causes vascular obstruction that leads to wilting, vascular necrosis and tree death within a few weeks [ 2 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

et al. 2018

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BackgroundThe Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus.ResultsWe present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC–MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors.ConclusionAnalysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4440-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

et al. 2018

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“…Em todos estes conidióforos é possível observar a saída dos esporos, indicando que neste período este fungo já está apto a produzir novos conídios. O mesmo pode ser observado no trabalho de Santos et al (18).…”

Section: Resultsunclassified

Colonização do xilema de eucalipto por Ceratocystis spp. isolado de diferentes hospedeiros

Firmino

Tanaka²,

Silva

et al. 2015

Summa phytopathol.

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(3), e C. fimbriata, causadora de doença em muitas plantas lenhosas, como em eucalipto (8). Novos estudos realizados por Van Wyk et al. (21), com vários isolados de manga de diferentes regiões, sugerem que Ceratocystis sp. is a fungus that colonizes the xylem and acts as the causal agent of wilt and drought in several woody plants. The present study aimed to monitor the colonization of this fungus on the surface of eucalyptus plants (Eucalyptus sp.

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“…Resultados semelhantes ao presente trabalho foram encontrados por Tumura (2011) que, segundo o autor, compostos fenólicos, tiloses e grãos de amido foram visualizados em maiores quantidades em plantas de eucalipto suscetíveis a Ceratocystis. Santos et al (2012) citam estes mesmo mecanismos de resistência em estudos relacionados com plantas de cacaueiro resistentes e suscetíveis inoculadas com C. cacaofunesta.…”

Section: Sciunclassified

Análise histológica de plantas de eucalipto resistentes e suscetíveis inoculadas com Ceratocystis fimbriata

Firmino¹,

Tozze²,

Viana³

et al. 2018

Sci. For.

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O gênero Ceratocystis contempla diversas espécies distribuídas em vários lugares do mundo. Uma planta infectada com este fungo apresenta como sintomas primários o escurecimento do sistema vascular e como sintomas secundários murcha, seca e morte da planta, decorrentes do colapso do xilema, causando pelo fungo. Estes sintomas são típicos de estresse hídrico e ocorre em plantas doentes como resultado do desenvolvimento do patógeno no interior de seus vasos e também por mecanismos de defesa da planta que bloqueiam vasos do xilema. O presente trabalho teve como objetivo realizar as análises das reações estruturais e bioquímicas que possam estar ligadas a resistência de plantas de eucalipto a Ceratocystis. Para observação das reações estruturais e bioquímicas das plantas de eucalipto à colonização do fungo, plantas de eucalipto de Eucalyptus grandis (Clone C3 resistente ao fungo e Clone C10 suscetível ao fungo) foram inoculadas com um isolado de C. fimbriata. Após a inoculação, fragmentos dos caules das plantas inoculadas foram coletados (7 e 21 dias após inoculação), fixados em "Karnovsky" e processadas para polimerização em historesina. Foram usadas diferentes técnicas para visualização de gomas e géis, grãos de amido, arginina, ligninas e tiloses. As tiloses, os géis e gomas foram observados em todas as plantas inoculadas com Ceratocystis. O acumulo de lignina foi observado somente em plantas resistentes. O amido e a arginina foram observados em todas as plantas trabalhadas. Estes resultados sugerem que a resistência de eucalipto ao fungo Ceratocystis pode estar ligada a capacidade de acumulo de ligninas nas células, já que esta estrutura somente foi visualizada em plantas resistentes inoculadas com o fungo.

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Kinetics and Histopathology of the Cacao-Ceratocystis cacaofunesta Interaction

Cited by 13 publications

References 22 publications

Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

Colonização do xilema de eucalipto por Ceratocystis spp. isolado de diferentes hospedeiros

Análise histológica de plantas de eucalipto resistentes e suscetíveis inoculadas com Ceratocystis fimbriata

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