Kinetics and bioenergetics of light‐limited photoautotrophic growth of <i>Spirulina platensis</i>

Lee, H. Y.; Erickson, Larry E.; Yang, Shouping

doi:10.1002/bit.260290705

Cited by 95 publications

(32 citation statements)

References 20 publications

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“…1. The phenomenon of decreasing apparent YG with increasing y and qe has also been observed in other laboratories (Gons & Mur, 1975;Eppley & Dyer, 1985;Lee et al, 1987) and in field studies (Oswald, 1970;Goldman, 1979;Richmond, 1983). Gon and Mur (1975) suggested that lower YG at high is a result of enhanced photorespiration, induced by high intracellular 02 partial pressure.…”

Section: Discussionsupporting

confidence: 73%

High CO2 partial pressure depresses productivity and bioenergetic growth yield of Chlorella pyrenoidosa culture

Lee

Tay

1991

J Appl Phycol

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When low-CO 2 grown Chlorellapyrenoidosa (YSK strain) cells were exposed to high CO 2 partial pressures (pCO 2 ), the specific growth rate () declined exponentially reaching a steady state value in about 20 h. A short interruption (up to 1 h) by temporarily lowering the pCO 2 did not prevent the continuous decline in y when high pCO 2 was restored. In chemostate studies, light-limited cultures were supplied with 2 to 90 kPa of CO 2 . The steady state biomass production rate and bioenergetic growth yield were related inversely to pCO 2 and the average energy uptake rate. The maintenance energy coefficient was, however, independent of dissolved pCO 2 in the pCO 2 range studied.

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Section: Discussionsupporting

confidence: 73%

High CO2 partial pressure depresses productivity and bioenergetic growth yield of Chlorella pyrenoidosa culture

Lee

Tay

1991

J Appl Phycol

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“…Its cultivation is still difficult because the available light energy is insufficient in low light, whereas photoinhibition occurs in high light. Mixotrophic or heterotrophic cultivation are appropriate ways to solve this problem (Lee et al 1987). However, Synechococcus sp.…”

Section: Introductionmentioning

confidence: 99%

Carbon metabolism and energy conversion of Synechococcus sp. PCC 7942 under mixotrophic conditions: comparison with photoautotrophic condition

et al. 2011

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To investigate the carbon metabolism and energy conversion efficiency of the cyanobacterium Synechococcus sp. PCC 7942 under mixotrophic conditions, we studied its growth characteristics in mixotrophic cultures with glucose and with acetate, respectively, and further discussed the carbon metabolism and energy utilization based on metabolic flux analysis. Results showed that both glucose and acetate could enhance the growth of Synechococcus sp. PCC 7942. The metabolic flux through the glycolytic pathway, tricarboxylic acid cycle, and mitochondrial oxidative phosphorylation was affected by the two organic substrates. Additionally, the cellular composition was also modulated by glucose and acetate. Under mixotrophic conditions, glucose exerts more significant impact on the diminishment of photochemical efficiency. Although the contribution of light energy was smaller, the cell yields based on total energy in mixotrophic cultures were higher compared with that of photoautotrophic one. On the basis of chlorophyll fluorescence analysis, the actual energy conversion efficiencies based on ATP synthesis in the photoautotrophic, glucose-mixotrophic, and acetatemixotrophic cultures were evaluated to be 4.59%, 5.86%, and 6.60%, respectively. Keywords Synechococcus sp. PCC 7942 . Mixotrophic cultivation . Carbon metabolism . Energy conversion . Metabolic flux analysis . Chlorophyll fluorescence analysis Nomenclature a ij Stoichiometric coefficient of metabolite i in the jth reaction (−) A m×nMatrix of stoichiometric coefficient (−) A 0 Side area of flask receiving incident light (m 2 ) E abLight energy absorbed by the biomass unit (kJ g −1 h −1 ) E P Actual light energy for photosynthesis (kJ g −1 h −1 ) E C Chemical energy received from organic carbon resources (kJ g −1 h −1 ) E C,ACE Chemical energy originated from acetate (kJ g −1 h −1 ) E C,GLC Chemical energy originated from glucoseThe total absorbed energy (kJ g −1 h −1 ) I 0 Incident light intensity (μmol m− 2 s −1 ) K a Effective absorption coefficient of light (m −1 ) rVector of m-dimensional metabolite accumulation rate (mmol g −1 h −1 ) r ATP Relative rate of ATP synthesized (mmol g −1 h −1 ) r ACE Relative rate of acetate consumption (mmol g −1 h −1 ) r ex Subvector of extracellular metabolite accumulation rate (mmol g −1 h −1 )Relative rate of glucose consumption (mmol g −1 h −1 ) r i Accumulation rate of metabolite i (mmol g −1 h −1 ) r in Subvector of intracellular metabolite accumulation rate (mmol gof cell mass based on ATP generation (g mol-ATP −1 ) Y E Cell yield based on total light energy (g kJ −1 ) ΔG o ACE Free energy change of acetate complete oxidation (kJ mol −1 ) ΔG o ATP Free energy change of ATP hydrolysis (kJ mol −1 ) ΔG o GLC Free energy change of glucose complete oxidation (kJ mol −1 ) μ Specific growth rate (h −1 ) 8 Vector of measurement noise variancecovariance (−) Φ PSII The efficiency of photosystem II photochemistry (−) Ψ ATP The efficiency of energy conversion on ATP synthesis (%)

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“…However, these values are valid only at low cell concentrations and a narrow light path, and give no information on local light energy distribution inside the culture. Thus, bioengineers have developed mathematical models to obtain the light distribution profile and average light intensity (Cornet et al, 1992;Lee et al, 1987;Molina Grima et al, 1997;Rabe and Benoit, 1962). Since the average light intensity can be identical for completely different conditions, the light distribution profile is required to describe the irradiance conditions more precisely.…”

Section: Introductionmentioning

confidence: 99%

A light distribution model for an internally radiating photobioreactor

Suh¹,

Lee²

2003

Biotech & Bioengineering

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Analysis of light energy distribution in culture is important for maximizing the growth efficiency of photosynthetic cells and the productivity of a photobioreactor. To characterize the irradiance conditions in a photobioreactor, we developed a light distribution model for a single-radiator system and then extended the model to multiple radiators using the concept of parallel translation. Mathematical expressions for the local light intensity and the average light intensity were derived for a cylindrical photobioreactor with multiple internal radiators. The proposed model was used to predict the irradiance levels inside an internally radiating photobioreactor using Synechococcus sp. PCC 6301 as a model photosynthetic microorganism. The effects of cell density and radiator number were interpreted through photographic and model simulation studies. The predicted light intensity values were found to be very close to those obtained experimentally, which suggests that the proposed model is capable of accurately interpreting the local light energy profiles inside the photobioreactor system. Due to the simplicity and flexibility of the proposed model, it was also possible to predict the light conditions in other complex photobioreactors, including optical-fiber and pond-type photobioreactors.

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Kinetics and bioenergetics of light‐limited photoautotrophic growth of Spirulina platensis

Cited by 95 publications

References 20 publications

High CO2 partial pressure depresses productivity and bioenergetic growth yield of Chlorella pyrenoidosa culture

High CO2 partial pressure depresses productivity and bioenergetic growth yield of Chlorella pyrenoidosa culture

Carbon metabolism and energy conversion of Synechococcus sp. PCC 7942 under mixotrophic conditions: comparison with photoautotrophic condition

A light distribution model for an internally radiating photobioreactor

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