Kinetic Vitrification of Spermatozoa of Vertebrates: What Can We Learn from Nature?

Katkov, I.; Bolyukh, V. F.; Chernetsov, O.A.; Dudin, P.I.; Grigoriev, A.Y.; Isachenko, Vladimir; Isachenko, Evgenia; Lulat, Ayub G.-M.; Moskovtsev, Sergey I.; Petrushkо, Maryna; Pinyaev, Vladimir I.; Sokol, K.M.; Sokol, Y.I.; Sushko, A. B.; Yakhnenko, Ilya

doi:10.5772/34784

Cited by 23 publications

(29 citation statements)

References 79 publications

(113 reference statements)

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“…This reaffirms the hypotheses proposed by Nauk (1991), Gao et al (1997), Nawroth et al (2002) and Katkov (2012) and 2) the presence of old spermatozoa, or those damaged during the process of cryopreservation, could generate reactive oxygen species that further damage sperm membranes and oxidise thiol groups, thus altering chromatin condensation, although this latter hypothesis is not supported by the findings of Guthrie and Welch (2006). With regard to the warming of vitrified samples, Gao and Critser (2000) and Johnson et al (2000) suggested that sperm survival could be decreased if it is carried out incorrectly.…”

Section: F I G U R Esupporting

confidence: 90%

“…According to Nawroth et al (2002) and Katkov (2012), the shape and size of the sperm head could be factors that define cell cryo-sensitivity. Comparative studies carried out by Nauk (1991) in sperm cryo-properties in different mammalian species (among which were human and porcine) showed a negative correlation between the size of the head and cryo-sensitivity.…”

Section: F I G U R Ementioning

confidence: 99%

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Porcine sperm vitrification I: cryoloops method

et al. 2016

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The aims of this study were to evaluate porcine sperm vitrification in cryoloops, with and without two different cryoprotectants and assess two warming procedures. Extended (n = 3; r = 4) and raw (n = 5; r = 2) semen was diluted in media without and with cryoprotectants (4% dimethylformamide and 4% glycerol) to a final concentration of 20 × 10 spermatozoa ml and vitrified using the cryoloops method. Two warming procedures were evaluated: rapid method (30 s at 37°C) and an ultra-rapid method (7 s at 75°C, followed by 30 s at 37°C). Total motility (phase contrast), sperm viability (6-carboxifluorescein diacetate and propidium iodide stain), membrane function (hypo-osmotic swelling test), acrosome integrity (phase contrast), chromatin condensation (toluidine blue stain) and chromatin susceptibility to acid denaturation (acridine orange stain) were evaluated before and after vitrification and analysed using Friedman's test. In all media, the only seminal parameters that were maintained after vitrification were chromatin condensation and integrity. Vitrification of porcine spermatozoon using cryoloops, both in the presence or absence of cryoprotectants and independent of the warming procedure used, permits conservation of sperm chromatin condensation and integrity. It would be interesting to further verify this by producing porcine embryos using vitrified spermatozoon with intracytoplasmic sperm injection.

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Section: F I G U R Esupporting

confidence: 90%

Section: F I G U R Ementioning

confidence: 99%

Porcine sperm vitrification I: cryoloops method

et al. 2016

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“…In contrast to the findings of Katkov et al .,38 our study showed that the highest post-thaw/warming motility and vitality resulted from cryopreservation using raffinose-based, rather than sucrose-based cryoprotectants. Also, with human spermatozoa,111239 successful post-warming outcomes were achieved after vitrification with 0.25 mol l −1 sucrose.…”

Section: Discussioncontrasting

confidence: 99%

Minimal volume vitrification of epididymal spermatozoa results in successful in vitro fertilization and embryo development in mice

et al. 2017

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This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling (18% w/v raffinose, RS-C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh control (FC) spermatozoa were compared with post-thawed or warmed RS-C, RV, and SV samples. Mouse oocytes (n = 267) were randomly assigned into three groups for insemination: RV (n = 102), RS-C (n = 86), and FC (n = 79). The number and the proportion of two-cell embryos and blastocysts from each treatment were assessed. Sperm motility (P < 0.01) and vitality (P < 0.05) were significantly reduced after vitrification compared with slow-cooled spermatozoa. However, DNA fragmentation was significantly reduced in spermatozoa vitrified using sucrose (15 ± 1.8% [SV] vs 26 ± 2.8% [RV] and 27 ± 1.2% [RS-C]; P < 0.01). Although the number of two-cell embryos produced by RS-C, RV, and FC spermatozoa was not significantly different, the number of blastocysts produced from two-cell embryos using RV spermatozoa was significantly higher than FC spermatozoa (P = 0.0053). This simple, small volume vitrification protocol and standard insemination method allows successful embryo production from small numbers of epididymal spermatozoa and may be applied clinically to circumvent the need for ICSI, which has the disadvantage of bypassing sperm selection.

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“…141,142,144,[147][148][149] Clinical efforts have further shown that vitrification of sperm in higher volumes (up to 0.5 mL) can be a practical and effective treatment achieving pregnancies and live births by IVF and intrauterine insemination (IUI) procedures. 141,142,145,147,[150][151][152][153][154] Although kinetic sperm vitrification has been proven effective for over 15 years, 5,147 it has not been met with the same large-scale acceptance and industry application afforded to eggs and embryos. However, as experimentation continues and more users become familiar with this technology, a trend away from traditional sperm freezing in the presence of toxic CPAs could well occur overtime.…”

Section: Human Sperm Vitrificationmentioning

confidence: 99%

“…4 Insights into this pioneering history of cryobiology and the early efforts of other investigators have been reviewed. 5 As for Luyet, he went on to become the founding president of the Society for Cryobiology in 1964.…”

Section: Insights Into the Pioneering Past Of Vitrificationmentioning

confidence: 99%

Vitrification: the pioneering past to current trends and perspectives of cryopreserving human embryos, gametes and reproductive tissue

Schiewe¹,

Anderson²

2017

BSAM

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After more than 2 decades of development in mammalian models and the clinical focus of a few pioneering laboratories, vitrification of human oocytes and embryos has transformed today's assisted reproductive technology (ART) industry. Our ability to cryopreserve gametes and embryos without fear of the damaging effects of ice formation has instilled great confidence in post-warming specimen viability. In turn, clinical treatment options are progressively eliminating fresh embryo transfer (ET; ie, freeze-all cycles), integrating preimplantation genetic screening and contemplating the use of cryopreserved oocytes as a viable resource. Vitrification's impact on clinical treatments is akin to the advent of sperm injection technology in the 1990s on male factor infertility. An appreciable and quantifiable difference was made, with procedural efficacy and global reliability essentially being guaranteed. Yet, there are challenges in the current trends and perspectives in how this technology is and will be optimized in the future. User variation in vitrification products and procedures warrants stricter adherence to quality control measures to enhance specimen biosafety and patient satisfaction, while reducing potential liability concerns. Furthermore, future progress in our understanding of the chemical and cryophysical processes of vitrification will insure the effective cryostorage of reproductive tissues and gametes at a level attained for embryo cryopreservation today.

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Kinetic Vitrification of Spermatozoa of Vertebrates: What Can We Learn from Nature?

Cited by 23 publications

References 79 publications

Porcine sperm vitrification I: cryoloops method

Porcine sperm vitrification I: cryoloops method

Minimal volume vitrification of epididymal spermatozoa results in successful in vitro fertilization and embryo development in mice

Vitrification: the pioneering past to current trends and perspectives of cryopreserving human embryos, gametes and reproductive tissue

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