Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Anion-Exchange Chromatography of Bovine Serum Albumin

Miyabe, Kanji; Guiochon, Georges

doi:10.1021/bp990062x

Cited by 45 publications

(26 citation statements)

References 48 publications

(85 reference statements)

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“…The plot of δd resembles that of the reciprocal of km,L (= δtotal). It is concluded that the concentration dependence of km,L results mainly from that of Ds because Dp is assumed to be independent of C. This situation is similar to those found in anion exchange 17,18 and reversed-phase chromatography. 27 A similar conclusion is derived from Fig.…”

Section: Explanation Of the Positive Concentration Dependence Of Kmlsupporting

confidence: 72%

“…The values of Ds are between 1 × 10 -8 and 1 × 10 -7 cm 2 s -1 . In previous papers, 17,18,27 we demonstrated a similar positive concentration dependence of Ds in two phase systems, in anion exchange and reversed-phase HPLC. In anion exchange chromatography of BSA, the heterogeneous surface model could represent the positive concentration dependence of Ds.…”

Section: Derivation Of An Estimate Of Dssupporting

confidence: 63%

“…dependence of both km,L of BSA 17,18 and km of Tröger's base 19 reported previously [8][9][10][11] and derived new information concerning the mass transfer characteristics of the four transport processes described above. In the case of anion exchange separation of BSA, 17,18 it was shown that surface diffusion played a predominant contribution in the kinetic properties of mass transfer and that the linear concentration dependence of km,L probably resulted from that of the surface diffusion coefficient (Ds).…”

supporting

confidence: 63%

“…In anion exchange chromatography of BSA, the heterogeneous surface model could represent the positive concentration dependence of Ds. 17,18 By contrast, in reversed-phase liquid chromatography (RPLC) on a C18-silica gel, the dependence of Ds of p-toctylphenol on its concentration was interpreted by the chemical potential driving force model. 27 Although Ds varies with the combination of solute, stationary and mobile phase in a separation system, some literature data on Ds previously reviewed are given in reference.…”

Section: Derivation Of An Estimate Of Dsmentioning

confidence: 99%

“…In the case of anion exchange separation of BSA, 17,18 it was shown that surface diffusion played a predominant contribution in the kinetic properties of mass transfer and that the linear concentration dependence of km,L probably resulted from that of the surface diffusion coefficient (Ds).…”

mentioning

confidence: 99%

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Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Enantiomeric Separation on a Polymeric Imprinted Stationary Phase

Miyabe

Guiochon

2000

ANAL. SCI.

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Molecular imprinting is a most attractive method of preparation of dedicated stationary phases for chromatography. Its use for enantioseparations is attracting considerable interest. 1,2 Imprinted materials can provide a very high selectivity for the objective compound, i.e., the imprinted molecule. [1][2][3][4][5] Although analytical applications are rapidly developing, the situation on the preparative front is less rosy. 1 Columns packed with imprinted stationary phases exhibit a poor efficiency and elution peaks are often unsymmetrical, especially for the more retained enantiomer. Sellergren et al. 1,2 suggested that the nonlinear behavior of the adsorption isotherm was the main cause of the broad and unsymmetrical bands observed for L-phenylalanine anilide (PA) on an L-PA imprinted chiral stationary phase. Although the possibility of a slow mass transfer kinetics was also pointed out, no detailed study supported this assumption. Yet, information on the mass transfer characteristics of imprinted stationary phases is necessary to clarify strategies for the improvement of these materials.Most kinetic studies in chromatography assume that four mass transfer processes are involved in a column, (1) axial dispersion, (2) fluid-to-particle mass transfer, (3) intraparticle diffusion, and (4) adsorption/desorption. 6 The contributions of these four processes to peak spreading are evaluated separately. Axial dispersion and the fluid-to-particle mass transfer resistance are inherent to flow processes through packed beds. They are well understood, relatively easy to control, and their contributions are often small. By contrast, intraparticle diffusion and adsorption/desorption give often larger contributions to the mass transfer resistance in the column. Their properties are intrinsic to each packing material and they are still often poorly understood. In order to evaluate the actual performance of packing materials, the characteristics of the mass transfer kinetics inside stationary phase particles must be clarified.Sajonz et al. 7 measured the adsorption equilibrium isotherm and the mass transfer kinetics of L-and D-PA on a polymeric stationary phase imprinted with L-PA, using a staircase frontal analysis method. The isotherm was well represented by either the Bi-Langmuir or the Freundlich models, but not by the simple Langmuir isotherm. The equilibrium data suggested that the distribution of the adsorption energy on the surface of the imprinted stationary phase is heterogeneous and showed that this phase has a high selectivity for the imprinted L-enantiomer. The lumped mass transfer rate coefficient (km,L) was calculated from each single breakthrough curve by applying the transport model of chromatography. 6 The values of km,L increased with increasing concentration of the enantiomers. The extent of the positive concentration dependence of km,L was larger for L-PA than for D-PA. It is expected that a more detailed analysis of the overall mass transfer rate parameter, km,L, could provide new, useful information and all...

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Section: Explanation Of the Positive Concentration Dependence Of Kmlsupporting

confidence: 72%

Section: Derivation Of An Estimate Of Dssupporting

confidence: 63%

supporting

confidence: 63%

Section: Derivation Of An Estimate Of Dsmentioning

confidence: 99%

mentioning

confidence: 99%

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Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Enantiomeric Separation on a Polymeric Imprinted Stationary Phase

Miyabe

Guiochon

2000

ANAL. SCI.

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Mass transfer and separation criteria for high‐speed countercurrent chromatography

Zhao¹,

He²

2011

AIChE Journal

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in Wiley Online Library (wileyonlinelibrary.com).High-speed countercurrent chromatography (HSCCC) is a versatile technique for preparative separations of a wide variety of solutes. For optimization of operating conditions, prediction of separations, and scale-up study, a model is needed to describe the effluent concentration profile, which determines the separation efficiency (mass transfer, mixing, and partitioning) and the resolution between peaks. A transferdispersive model is proposed to describe the effluent profile based on the assumption that the retention of a peak is caused by partitioning over two phases, and peak broadening is caused by axial dispersion and mass transfer limitation. In this work, mass transfer was investigated by comparing model simulations to experimental data. One generalized correlation of overall mass transfer coefficients was derived. Based on the correlations of axial dispersion coefficients in our previous work and mass transfer coefficients in this study, the model predicts the elution concentration profile well. Furthermore, separation criteria were proposed to predict the separation of two adjacent solutes, and they were verified using literature data.

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Adsorption of α‐amylase in a fixed bed: Operating efficiency and kinetic modeling

2003

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Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Anion-Exchange Chromatography of Bovine Serum Albumin

Cited by 45 publications

References 48 publications

Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Enantiomeric Separation on a Polymeric Imprinted Stationary Phase

Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Enantiomeric Separation on a Polymeric Imprinted Stationary Phase

Mass transfer and separation criteria for high‐speed countercurrent chromatography

Adsorption of α‐amylase in a fixed bed: Operating efficiency and kinetic modeling

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