Kinetic study of the chemical behavior of quercetin and Kaempferol in alkaline media

Tzouwara-Karayanni, Stella M.; Philianos, S.

doi:10.1007/bf01204593

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…2,3 The determination of flavonoids at the 10 mM level has generally been carried out by spectrofluorometric methods and measuring the intrinsic fluorescence of these compounds. 4 Sensitive methods have been used to determine lower concentrations of flavonoids (mainly morin) based on the formation of a fluorescent complex with ethonium 5 or spectrofluometrically following the oxidation of flavonoids at alkaline pH 6 (with determination ranges of 2.5-25 mmol dm -3 for both morin and kaempferol).…”

Section: Introductionmentioning

confidence: 99%

Kinetic determination of morin nanoamounts

Kostić

Mitić

Miletic

2004

JSCS

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kinetic-spectrophotometric method is proposed for the determination of morin. The method is based on the inhibition effect of morin on the oxidation of C6H5COON aby hydrogen peroxide in the presence of the complex Fe(II)-AA(ascorbic acid),which acts as a catalyst. The concentration range for the determination of morin is one of the lowest achieved so far (a linear calibration graph was obtained for morin from 2.255?22.55 ng cm-3). The limit of detection of the method is 0.28 ng cm-3. The relative error ranges between 1.42 to 5.10% for the given concentration interval. Kinetic equations are proposed for the investigated process. The effects of certain foreign ions upon the reaction rate were determined in order to assess the selectivity of the method. The major advantages of this kinetic-spectrophotometric assay are its sensitivity, selectivity, reproducibility, speed and simplicity.

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Section: Introductionmentioning

confidence: 99%

Kinetic determination of morin nanoamounts

Kostić

Mitić

Miletic

2004

JSCS

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Protic equilibria as the key factor of quercetin emission in solution. Relevance to biochemical and analytical studies

Mezzetti

Protti

Lapouge

et al. 2011

Phys. Chem. Chem. Phys.

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A detailed spectrofluorimetric study on quercetin in aqueous solution proves that its anionic forms are responsible for a strong fluorescence enhancement observed at pH > 6. Anion fluorescence is also observed in organic solvents with strong hydrogen bond acceptor properties. The results provide a new interpretation of biophysical and analytical literature data where the fluorescence of the anionic forms of quercetin has never been explicitly taken into account. In particular, comparison with published binding studies strongly suggests that quercetin deprotonation and consequent fluorescence enhancement takes place when the flavonoid binds to several biological macromolecules. This observation can be very helpful in the understanding at a molecular level of the interaction between quercetin and the biomolecule.

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Kinetic study of the chemical behavior of quercetin and Kaempferol in alkaline media

Cited by 2 publications

References 4 publications

Kinetic determination of morin nanoamounts

Kinetic determination of morin nanoamounts

Protic equilibria as the key factor of quercetin emission in solution. Relevance to biochemical and analytical studies

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