Kinetic Study of the Cetyltrimethylammonium/DNA Interaction

Abstract

A kinetic study of the interaction of the surfactant cetyltrimethylammonium (CTA(+)) with DNA was carried out in water and in salt (NaCl) solutions. The results can be explained in terms of a reaction mechanism involving two consecutive reversible steps. The first step corresponds to the union/separation of the surfactant with/from the DNA. The second step corresponds to a conformational change of the surfactant/DNA complex. The equilibrium constant, calculated from the forward and reverse rate constants of these steps, agrees with the results of a previous thermodynamic study.

Improving the understanding of DNA–propanediyl-1,3-bis(dodecyldimethylammonium) dibromide interaction using thermodynamic, structural and kinetic approaches

“…2 Motivated by the promising application of cationic surfactants in gene delivery, several groups have explored the interactions of these vectors with nucleic acids (DNA and RNA). [3][4][5][6][7] More specifically, gemini surfactants have received a lot of attention recently due to their unique physicochemical properties, low cytotoxicity and potential bactericidal properties. [8][9][10][11] These types of surfactants, composed of two hydrophobic chains and two hydrophilic head groups covalently attached through a spacer, present high compaction efficiency in comparison with their conventional counterparts that bear single head groups and monoquaternary chains.…”

Supramolecular Systems Based on Novel Mono‐ and Dicationic Pyrimidinic Amphiphiles and Oligonucleotides: A Self‐Organization and Complexation Study

“…Deoxyribonucleic acid (DNA) complexation with cationic ligands is of fundamental and practical importance and has long been the subject of many investigations. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] DNA condensation is a key phenomenon in living matter, therefore the information on interactions between DNA and cationic condensing agents may contribute to a better understanding of the DNA behavior in living cells. [2,[15][16][17] Besides, DNA compaction induced by gene vectors is a crucial step in gene therapy, [9,18,19] which is based on the use of nucleic acids as therapeutic agents for the treatment of human diseases.…”

A simple model for the behavior of multisite receptors in photochemical processes

“…Fig. 4 represents the t of experimental data of mixtures of class i to eqn (11). According to this result, a receptor containing two different types of binding sites cannot be distinguished, employing only data of emission intensity, from a receptor containing only one kind of binding site.…”

“…9 Of course, this modies the characteristics of the biological molecule as receptor. 10,11 Moreover, cooperativity effects are frequent in the case of biological systems. [12][13][14][15] These effects obviously change the characteristics of the binding sites, increasing or decreasing the affinity of the ligand by the receptor 16 (positive or negative cooperativity).…”