Kinetic study of sulphuric acid hydrolysis of protein feathers

Bouhamed, Sana Ben Hamad; Kechaou, Nabil

doi:10.1007/s00449-017-1737-7

Cited by 25 publications

(16 citation statements)

References 21 publications

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“…As mentioned above, slaughterhouses produce millions of tons of keratin-containing biomass as co-product every year ( Sharma and Gupta, 2016 ; Li et al, 2020 ). Keratin biomass is extremely rich in protein; for example, ~90% of feather dry matter is protein ( Ben Hamad Bouhamed and Kechaou, 2017 ), while wool contains up to 95% by weight of pure keratin ( Eslahi et al, 2013 ).…”

Section: Essentials Of Keratin Biomass As Substrate For Keratinolyticmentioning

confidence: 99%

Microbial enzymes catalyzing keratin degradation: Classification, structure, function

Qiu

Wilkens

Barrett

et al. 2020

Biotechnology Advances

141

103

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Keratin is an insoluble and protein-rich epidermal material found in e.g. feather, wool, hair. It is produced in substantial amounts as co-product from poultry processing plants and pig slaughterhouses. Keratin is packed by disulfide bonds and hydrogen bonds. Based on the secondary structure, keratin can be classified into α-keratin and β-keratin. Keratinases (EC 3.4.-.- peptide hydrolases) have major potential to degrade keratin for sustainable recycling of the protein and amino acids. Currently, the known keratinolytic enzymes belong to at least 14 different protease families: S1, S8, S9, S10, S16, M3, M4, M14, M16, M28, M32, M36, M38, M55 (MEROPS database). The various keratinolytic enzymes act via endo -attack (proteases in families S1, S8, S16, M4, M16, M36), exo -attack (proteases in families S9, S10, M14, M28, M38, M55) or by action only on oligopeptides (proteases in families M3, M32), respectively. Other enzymes, particularly disulfide reductases, also play a key role in keratin degradation as they catalyze the breakage of disulfide bonds for better keratinase catalysis. This review aims to contribute an overview of keratin biomass as an enzyme substrate and a systematic analysis of currently sequenced keratinolytic enzymes and their classification and reaction mechanisms. We also summarize and discuss keratinase assays, available keratinase structures and finally examine the available data on uses of keratinases in practical biorefinery protein upcycling applications.

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Section: Essentials Of Keratin Biomass As Substrate For Keratinolyticmentioning

confidence: 99%

Microbial enzymes catalyzing keratin degradation: Classification, structure, function

Qiu

Wilkens

Barrett

et al. 2020

Biotechnology Advances

141

103

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“…The feathers were freshly collected from a poultry processing unit in Sfax (Tunisia), washed fully with distilled water and dried at 90°C for 5 h. The FPH was prepared by the method of Ben Hamad Bouhamed et al. , with some modifications. It was conducted in jacketed glass reactor with aqueous H 2 SO 4 (0.5 N) and feather: solvent ratio (w/v) of 1:20 at 90°C for 100 min.…”

Section: Methodsmentioning

confidence: 99%

“…Feathers represent the major by‐product generated from poultry processing plants with 10% of the total weight of the poultry, and they constitute an important source of proteins (85%) . The antioxidant activity of feather protein hydrolysates (FPH) was previously investigated .…”

Section: Introductionmentioning

confidence: 99%

Antihyperlipidemic and antioxidant effects of feather protein hydrolysate in high‐fat diet‐fed mice

Bouhamed

Chaari

Bellassoued

et al. 2019

Engineering in Life Sciences

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The hyperlipidemia is a serious health problem that increases the risk of many complications including cardiovascular disease. This study aims to evaluate the possible antihyperlipidemic effects of the feather protein hydrolysate (FPH) in a mice fed with a high‐fat diet (HFD)‐fed mice during 5 weeks. The FPH administration improved dose‐dependent lipid profile, as well as the liver and renal dysfunction indices in hyperlipidemic mice. The FPH also restored the antioxidant status in liver, kidney, and heart by lowering the lipid peroxidation and enhancing the antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase [SOD]). Moreover, the histological studies proved that FPH administration prevents hepatic steatosis, glomerular hyperfiltration risk, and cardiac muscle hypertrophy. Accordingly, the FPH is a promising novel medicinal ingredient for possible use in the hyperlipidemic treatment and related complications.

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“…(Sinkiewicz et al 2018). Regrettably, commonly applied hydrolysis using a strong mineral acid (Bouhamed and Kechaou 2017) or base (Pahua-Ramos et al 2017) leads to a necessary subsequent recycling of the process solutions, including neutralization and elimination of undesirable salts. In the literature, there are now dozens of studies mostly focused on the biotransformation of feathers by microorganisms or enzymes, unfortunately mostly in sophisticated treatments suitable for laboratories (Kumar et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Environmental aspects and economic evaluation of new green hydrolysis method for waste feather processing

Šolcová

Knápek

Wimmerová

et al. 2021

Clean Techn Environ Policy

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In recent years, an increase in the chicken meat process industry has been growing quickly, which brings a large amount of difficult to process waste, chicken feathers. These billions of kilograms create a serious waste problem over the world, furthermore, poultry feather processing is not only particularly difficult but also relatively expensive. In fact, there is no technology suitable for processing such huge quantities of feathers to guarantee sustainable development of the chicken meat industry together with processing of waste feathers. This article introduces a newly designed and original, highly efficient and environmentally friendly method of physicochemical hydrolysis of waste feathers. The hydrolysis is carried out in the presence of a weak organic carboxylic acid; thus, the resulting hydrolysate does not contain any salts or ashes. Therefore, a resulting hydrolysate, which includes a mixture of amino acids, peptides, proteins, glycoproteins and free fatty acids, is suitable for a variety of applications; e.g. as a chondroprotective agent in the treatment of joint diseases, nutrients for crop plants or targeted biostimulants for agriculture. This paper is focused not only on the process scale-up but also on environmental aspects and economic evaluations to bring general view of the process. Graphic abstract

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Kinetic study of sulphuric acid hydrolysis of protein feathers

Cited by 25 publications

References 21 publications

Microbial enzymes catalyzing keratin degradation: Classification, structure, function

Microbial enzymes catalyzing keratin degradation: Classification, structure, function

Antihyperlipidemic and antioxidant effects of feather protein hydrolysate in high‐fat diet‐fed mice

Environmental aspects and economic evaluation of new green hydrolysis method for waste feather processing

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