Kinetic studies of phenol degradation by Rhodococcus sp. P1 II. Continuous cultivation

Abstract

The degradation of phenol by Rhodococcus sp. P1 was studied in continuous culture systems. The organism could be adapted by slowly increasing concentration, step by step, up to 30.0 g.l-1 phenol in the influent. The degradation rate reached values of about 0.3 g.g dry mass-1.h-1. Large step increases in phenol concentration and addition of further substrates (e.g., catechol) were tolerated up to a certain concentration. With increasing dilution rate and increasing inlet phenol concentration the stability of the system decreased. Nomenclature: D--Dilution rate, h-1, Dc--Critical dilution rate, h-1, Dx--Yield, g dry mass.l-1.h-1, Ks--Monod saturation constant, g.l-1, S--Growth-limiting substrate concentration in culture broth, g.l-1, SR--Growth-limiting substrate concentration in feed, g.l-1, mean--Biomass concentration in culture broth, g.l-1, YS--Yield constant, g cell dry mass.g substrate-1, mu--Specific growth rate, h-1, mu max--Maximum growth rate, h-1.

Isolation and process parameter optimization of Brevibacterium casei for simultaneous bioremediation of hexavalent chromium and pentachlorophenol

“…Further, in order to achieve high removal efficiencies, bacteria were acclimatized by growing in successive increasing concentration of Cr 6+ and PCP as the sole carbon and energy source. This could be probably due to a marked increase in resistance among bacterial cells when continuously exposed to toxic chemicals . Such an increase in resistance may translate into enhancement of bacterial metabolic rate, which in our case was detected as a remarkable increment in the simultaneous detoxification of chromate and PCP.…”

Combined effects of external mass transfer and biodegradation rates on removal of phenol by immobilized Ralstonia eutropha in a packed bed reactor

“…Also phenol biodegradation by suspended cells of Rhodococcus sp. P1 in continuous culture systems [22] and Rhodococcus sp. immobilized in calcium-alginate beads or on granular activated carbon [23] have been reported.…”

Degradation kinetics and pathway of phenol byPseudomonasandBacillusspecies

“…Studies have shown that phenol can be aerobically degraded by a wide variety of microorganisms of genera Pseudomonas ,[ 15–2 2] Acinetobacter ,[ 23 ] Alcaligenes ,[ 24 , 25 ] Bacillus ,[ 26 ] Burkholderia ,[ 27 , 28 ] Nocardia ,[ 29 ] Nocardioides ,[ 30 ] Ralstonia [ 31 ] and Rhodococcus . [ 32 ] In this study, we have presented the pathway and degradation kinetics of phenol by Pseudomonas and Bacillus species.…”