Kinetic Spectrophotometric Determination of Famotidine in Pharmaceutical Preparations

Abstract

A simple accurate kinetic spectrophotometric method was developed for the determination of biotin in pure form and pharmaceutical preparations. The proposed method is based on a catalytic acceleration of biotin on the reaction between sodium azide and tri-iodide in an aqueous solution. Concentration range of 4-16 μg/mL for biotin was determined by measuring the decrease in the absorbance of tri-iodide at 348 nm by a fixed time method. The decrease in absorbance after 14 min from the initiation of the reaction was markedly correlated to the concentration with correlation coefficient of 0.9999. The detection limit (LOD) of biotin was 0.18 μg/mL while quantitation limit (LOQ) was 0.54 μg/mL. The percentage recovery of the spiked samples was 100.08 ± 0.761. The proposed procedure was successfully applied for the determination of biotin in its pharmaceutical preparations with mean recoveries of 101.17 ± 2.05 and 97.87 ± 1.50 for biotin ampoules and capsules, respectively. The results obtained were in good agreement with those obtained using official method.

Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas

“…Dentre os métodos descritos estão a eletroforese capilar com detecção no UV, [6][7][8][9] potenciometria, 10 voltametria, 11 espectrofotometria, 12-17 cromatografia líquida de alta eficiência (CLAE) [18][19][20][21][22][23][24][25][26][27][28][29][30][31] e análise por injeção em fluxo. [32][33][34] As Farmacopeias Brasileira e Americana registram a monografia para famotidina na forma farmacêutica comprimido com quantificação por CLAE. 35,36 No entanto, para identificação do fármaco é utilizada a varredura espectral com solução de tampão fosfato pH 2,5, 35 e, ainda, no ensaio de dissolução para comprimidos a quantidade de fármaco liberado da forma farmacêutica é quantificada por espectrofotometria utilizando-se solução de tampão fosfato pH 4,5.…”

Stability indicating method for famotidine in pharmaceuticals using porous graphitic carbon column

“…Thus, the analytical activities concerning impurities in drugs are among the most important issues in modern pharmaceutical analysis. Several analytical techniques such as spectrophotometry [4 -8], potentiometry and electrogenerated chemiluminescence [9,10], flow injection analysis [11,12], CE [13 -15] and more extensively HPLC [16 -23] have been reported for the individual and simultaneous determination of FMT and other antihistaminic analogues. However, among the most extensively used HPLC methods, only few of them describe the simultaneous determination of FMT and related compounds [24 -32].…”

Determination of Labetalol Hydrochloride in Drug Formulations by Spectrophotometry

“…UV-visible spectrophotometry is the technique of choice even today in the laboratories of research, hospitals and pharmaceutical industries due to its low cost and inherent simplicity. The analytical procedures based on spectrophotometry [17][18][19][20][21][22] have been fre-quently published in reputed journals. Moreover, spectrophotometry is a well known technique which has been utilized for the determination of isoniazid based on the reaction of drug with 1,2-naphthoquinone-4-sulfonic acid sodium in alkaline medium (pH 13).…”