Kinetic Retrieval of Eosinophil Cationic Protein, Hyaluronan, Secretory IgA, Albumin, and Urea During BAL in Healthy Subjects

Schmekel, Birgitta; Hörnblad, Y; Hvatum, M; Norlund, Ann-Louise

doi:10.1378/chest.108.1.62

Cited by 15 publications

(11 citation statements)

References 26 publications

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“…We also found, as shown before by others (44,45), that tracheal secretions contain HA. Interestingly, we found that NHBE cells released HA into the apical and BL compartments.…”

Section: Discussionsupporting

confidence: 92%

Identification of Glycosaminoglycans in Human Airway Secretions

Monzón

Casalino‐Matsuda

Forteza

2006

Am J Respir Cell Mol Biol

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Glycosaminoglycans (GAGs), known to be present in airway mucus, are macromolecules with a variety of structural and biological functions. In the present work, we used fluorophore-assisted carbohydrate electrophoresis (FACE) to identify and relatively quantify GAGs in human tracheal aspirates (HTA) obtained from healthy volunteers. Primary cultures of normal human bronchial epithelial (NHBE) and submucosal gland (SMG) cells were used to assess their differential contribution to GAGs in mucus. Distribution was further assessed by immunofluorescence in human trachea tissue sections and in cell cultures. HTA samples contained keratan sulfate (KS), chondroitin/dermatan sulfate (CS/DS), and hyaluronan (HA), whereas heparan sulfate (HS) was not detected. SMG cultures secreted CS/DS and HA, CS/DS being the most abundant GAGs in these cultures. NHBE cells synthesized KS, HA, and CS/DS. Confocal microscopy showed that KS was exclusively found at the apical border of NHBE cells and on the apical surface of ciliated epithelial cells in tracheal tissues. CS/DS and HA were present in both NHBE and SMG cells. HS was only found in the extracellular matrix in trachea tissue sections. In summary, HTA samples contain KS, CS/DS, and HA, mirroring a mixture of secretions originated in surface epithelial cells and SMGs. We conclude that surface epithelium is responsible for most HA and all KS present in secretions, whereas glands secrete most of CS/DS. These data suggest that, in diseases where the contribution to secretions of glands versus epithelial cells is altered, the relative concentration of individual GAGs, and therefore their biological activities, will also be affected.

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“…We also found, as shown before by others (44,45), that tracheal secretions contain HA. Interestingly, we found that NHBE cells released HA into the apical and BL compartments.…”

Section: Discussionsupporting

confidence: 92%

Identification of Glycosaminoglycans in Human Airway Secretions

Monzón

Casalino‐Matsuda

Forteza

2006

Am J Respir Cell Mol Biol

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“…HA is a glycosaminoglycan that is normally found as a high molecular weight polymer attached to the apical membrane of bronchial epithelium (20,21), soluble in tracheobronchial secretions (20,66,67) and in the subepithelial matrix (68). HA is involved in a broad range of processes that involve structural changes in AJC.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan and Layilin Mediate Loss of Airway Epithelial Barrier Function Induced by Cigarette Smoke by Decreasing E-cadherin

Forteza

Casalino‐Matsuda

Falcon

et al. 2012

Journal of Biological Chemistry

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Background: Cigarette smoke (CigS) induces hyaluronan fragmentation and increases epithelial permeability. Results: CigS and HA fragments decrease E-cadherin expression that is prevented by knocking down layilin. Conclusion: HA fragments bind to layilin and signal through RhoA/ROCK to inhibit E-cadherin. Significance: Airway epithelium is our first line of defense against inhaled insults. HA fragments released by CigS disrupt this barrier.

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“…For the measurements of soluble markers such as ECP the fluid has to be processed immediately by separation of the cells at 4 °C followed by storage in a freezer at − 20 °C. Differences in the lavage procedure with respect to volumes and dwell times have made the comparison of ECP data from different studies very difficult [ 71, 72]. In particular the use of large lavage volumes often results in undetectable ECP levels due to dilution of the material.…”

Section: Ecp Measurements In Various Body Fluidsmentioning

confidence: 99%