2003
DOI: 10.1113/jphysiol.2003.052142
|View full text |Cite
|
Sign up to set email alerts
|

Kinetic properties of the α2 homo‐oligomeric glycine receptor impairs a proper synaptic functioning

Abstract: Ionotropic glycine receptors (GlyRs) are present in the central nervous system well before the establishment of synaptic contacts. Immature nerve cells are known, at least in the spinal cord, to express α2 homomeric GlyRs, the properties of which are relatively unknown compared to those of the adult synaptic form of the GlyR (mainly α1/β heteromeres). Here, the kinetics properties of GlyRs at the single‐channel level have been recorded in real‐time by means of the patch‐clamp technique in the outside‐out confi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

7
83
0

Year Published

2004
2004
2018
2018

Publication Types

Select...
6
2

Relationship

1
7

Authors

Journals

citations
Cited by 69 publications
(90 citation statements)
references
References 52 publications
7
83
0
Order By: Relevance
“…Together, these properties of glycinergic transmission suggest that it is well suited for modifying excitation with temporal precision. Given that IPSC decay kinetics at room temperature also exceeded those reported for receptors in patches excised from neurons (Singer and Berger, 1999;Ali et al, 2000) or from cells expressing human or zebrafish ␣1 subunits (Fucile et al, 1999;Gentet and Clements, 2002) or ␣2 subunits (Mangin et al, 2003), it seems likely that the receptors in mature MNTB contain a novel subunit or posttranslational modification.…”
Section: Glycinergic Transmission In Mntbmentioning
confidence: 99%
“…Together, these properties of glycinergic transmission suggest that it is well suited for modifying excitation with temporal precision. Given that IPSC decay kinetics at room temperature also exceeded those reported for receptors in patches excised from neurons (Singer and Berger, 1999;Ali et al, 2000) or from cells expressing human or zebrafish ␣1 subunits (Fucile et al, 1999;Gentet and Clements, 2002) or ␣2 subunits (Mangin et al, 2003), it seems likely that the receptors in mature MNTB contain a novel subunit or posttranslational modification.…”
Section: Glycinergic Transmission In Mntbmentioning
confidence: 99%
“…To make a "sniffer" electrode (Young and Poo, 1983), an outside-out patch was pulled from a transfected CHO cell line expressing the GlyR ␣2 subunit (Mangin et al, 2003). Homomeric GlyR ␣2 subunits, highly specific for glycine, were characterized by a high elementary conductance (100 -120 pS), a long mean open time, and little desensitization, making them a good sensor for glycine (Mangin et al, 2003). As a control, the electrode was first positioned outside the SC to verify any glycine contamination in the recording medium.…”
Section: Methodsmentioning
confidence: 99%
“…For electrophysiological recordings, cells were seeded onto glass coverslips coated with poly-L-ornithine (0.1 mg/ml). Glycine receptor ␣ 2 subunit cloning and transfection were performed as described by Mangin et al (2003).…”
Section: Methodsmentioning
confidence: 99%
“…These problems will be the subject of future work, but preliminary results suggest that experiments simulated with the flip mechanism can be fitted very well with scheme 3 (18 free parameters) and that results simulated with scheme 3 can be fitted with the flip mechanism (14 free parameters) in a way that is not perfect but is sufficiently good that it would not be distinguishable in practice, with the sort of recordings that we have made. Possible ways of distinguishing between the rival mechanisms include looking at a range of agonists (in progress), recording single channels after a concentration jump, increasing resolution by using ultra-low noise methods, decreasing temperature, or using the slow ␣2 subunit (Mangin et al, 2003).…”
Section: Mechanism Versus Descriptionmentioning
confidence: 99%