Kinetic Properties of Purified β-Mannanase from Penicillium italicum

Olaniyi, Oladipo Oladiti

doi:10.9734/bmrj/2014/6555

Cited by 14 publications

(18 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mannanase has been produced by various microorganisms and purified as reported in previous studies. Olaniyi et al [1] produced and purified mannanase enzyme from Penicillium italicum isolated from yam peel. Madau & Shetati sourced for β-1, 4-mannanase from Scopulariosis candida and purified it while Sumardi isolated Geobacillus stearothermophilus L07 from oil palm shell, screened for mannanase production after which it was purified.…”

Section: Discussionmentioning

confidence: 99%

“…In the first stage, the supernatant obtained after centrifugation was precipitated by the addition of ammonium sulphate to achieve 70% ammonium sulphate concentration [1]. The precipitated enzyme was then diluted in phosphate buffer 50 mM pH 7.0, and dialyzed in the same buffer.…”

Section: Mannanase Purificationmentioning

confidence: 99%

“…Beta-mannanase otherwise known as mannan endo-1,4-β-mannosidase or 1,4-β-D-mannanase with an enzyme commission (EC) 3.2.1.78 breakdowns β-1,4-mannosidic bonds in the main chain of glucomannans, galactomannans and β-1,4-mannans [1]. It transforms the abundant mannan-rich heteromannan, glucomannan and galactomannan substrates into mannooligosaccharides [2] and a small amount of mannose, glucose and galactose [1,3].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…It transforms the abundant mannan-rich heteromannan, glucomannan and galactomannan substrates into mannooligosaccharides [2] and a small amount of mannose, glucose and galactose [1,3]. A number of plants, bacteria, fungi and various invertebrates had been reported to produce β-mannanase [1,3]. The mannanase of biological origins have found a variety of applications in different industrial sectors [3], including animal feed formulation, pharmaceutical products preparation, pulp biobleaching, [4], as well as pre-treatment of plant biomass for second generation biofuel production [5].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Untitled

2017

JBMOA

View full text Add to dashboard Cite

The present study aimed at the isolation, screening and partial purification of beta-mannanase from fungi isolated from soil and water samples collected from Ilaje Lake, Ondo state, Nigeria. The associated fungi were isolated and counted by standard microbiological methods. Partial purification of crude mannanase was done by standard biochemical methods. Quantitatively, mannanase production was performed in mineral salt medium into which Locust Bean Gum (LBG) had been incorporated as the sole carbon source. Enzyme activity was determined by dinitrosalicylic acid (DNSA) method, while protein content was evaluated by Lowry's method. The highest fungal counts were recorded for water sample collected from Ilaje Lake with 3.4×10 8 sfu/mL. The organisms encountered include Aspergillus flavus, Rhizopus stolonifer, A. fumigatus, A. niger, R. japonicus, Penicillum italicum, Fusarium solani and Candida albicans. All the fungal isolates encountered from these sources showed varied degrees of mannanase activities. The highest specific mannanase activity was recorded for isolate 4B1, while the lowest value was obtained with isolate 1B2. Purification of crude mannanase from A. niger was carried out by ammonium sulphate precipitation and gel filtration . Fractionation of ammonium sulphate precipitated mannanase from A. niger on Sephadex G-200 produced two activity peaks. In this investigation, fungal isolates evaluated for mannanase production from this source gave appreciable mannanase activity and this could be applied in many industrial processes. Keywords: Materials and Methods Sources of SampleWater and soil samples were collected from Ilaje Lake, Ondo State, Nigeria in sterile bottles. The samples were transferred to the laboratory and were used as sources for the isolation of mannanase-producing fungi [9,10]. Isolation and identification of mannanase-producing fungi from soil and water samplesThe total fungal counts from the samples were determined using the pour plate method on potato dextrose agar. The samples were serially diluted and 1ml of an appropriate dilution was used to inoculate the plate in duplicate. The plates were incubated at 28 ± 2˚C for 72hrs, after which the total colony count was determined as previously described [11]. At the end of incubation, the colonies were sub-cultured from the mixed cultures and identified on the bases of cultural characters (colour, shape of colony, surface and reverse pigmentation and texture of the colony) as well as microscopic structure (septate or nonseptate hyphae, structure of hyphae and conidia) [12]. Screening of mannanase-producing fungi in submerged state fermentationMedium composition described by Mandles & Weber [13] modified by Arotupin & Olaniyi [10] was used for submerged fermentation (static condition). The basal medium contained (g/L): LBG 10g, peptone 2g, yeast extract 2g, NaNO 3 2 g, KH 2 PO 4 1 g, MgSO 4 .7H 2 O 0.5 g, KCl 0.5 g and FeSO 4 .7H 2 O traces. The pH of the media was adjusted to 6.8 with pH meter (Denver Instrument, Model 20 pH/ Co...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Mannanase Purificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Untitled

2017

JBMOA

View full text Add to dashboard Cite

show abstract

Understanding β‐mannanase from Streptomyces sp. CS147 and its potential application in lignocellulose based biorefining

Yoo

Pradeep

Lee

et al. 2015

Biotechnology Journal

View full text Add to dashboard Cite

Hydrolytic enzymes such as cellulase and hemicellulase have been attracted in lignocellulose based biorefinery. Especially, mannanase has been a growing interest in industrial applications due to its importance in the bioconversion. In this study, an extracellular endo-β-1,4-D-mannanase was produced by Streptomyces sp. CS147 (Mn147) and purified 8.5-fold with a 43.4% yield using Sephadex G-50 column. The characterization of Mn147 was performed, and the results were as follows: molecular weight of ∼25 kDa with an optimum temperature of 50°C and pH of 11.0. The effect of metal ions and various reagents on Mn147 was strongly activated by Ca(+2) but inhibited by Mg(+2) , Fe(+2) , hydrogen peroxide, EDTA and EGTA. Km and Vmax values of Mn147 were 0.13 mg/mL and 294 μmol/min mg, respectively, when different concentrations (3.1 to 50 mg/mL) of locust bean gum galactomannan were used as substrate. In enzymatic hydrolysis of heterogeneous substrate (spent coffee grounds), Mn147 shows a similar conversion compared to commercial enzymes. In addition, lignocellulosic biomass can be hydrolyzed to oligosaccharides (reducing sugars), which can be further utilized for the production of biomaterials. These results showed that Mn147 is attractive in quest of potential bioindustrial applications.

show abstract