Kinetic properties and adrenergic control of TREK-2-like channels in rat medial prefrontal cortex (mPFC) pyramidal neurons

Ładno, W.; Gawlak, Maciej; Szulczyk, Paweł; Nurowska, Ewa

doi:10.1016/j.brainres.2017.04.009

Cited by 7 publications

(20 citation statements)

References 80 publications

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“…In the study, we investigated the effect of divalent ions and temperature on the amplitude of TREK-2-like currents that appeared spontaneously in membrane depolarization and hyperpolarization. Identification of the channel as a TREK-2-like channel in its non-canonical isoform (b or c) has been described previously [6] and was performed on the basis of pharmacological and biophysical properties and with the use of immunofluorescence together with confocal microscopy. Non-canonical isoforms result from alternative splicing [7].…”

Section: Resultsmentioning

confidence: 99%

“…www.journals.viamedica.pl/medical_research_journal in rat pyramidal prefrontal cortex neurons. These currents have been previously identified as conducted by TREK-2-like channels in its non-canonical form b or c [6]. We performed experiments with two different solutions in a pipette (pipette solution corresponds to the extracellular environment).…”

Section: Resultsmentioning

confidence: 99%

“…The experiments were performed on young (20-days-old) male Wistar rats. Preparation of the slices and the procedure of enzymatic tissue dissociation were performed as described previously [6]. Voltage-clamp recordings were performed in the cell-attached configuration using an Axopatch 1D amplifier (Axon Instruments) and pClamp 9.0 software.…”

Section: Methodsmentioning

confidence: 99%

“…The main function of leak potassium channels is to stabilize the resting membrane potential, which affects the excitability of neurons. It was recently reported that TREK-2-like channels are present in pyramidal cells in the prefrontal cortex of rats [6]. Thus, the excitability of prefrontal pyramidal cells can depend on the activity of TREK-2-like channels, not only in physiological but also in pathophysiological conditions.…”

Section: Introductionmentioning

confidence: 96%

“…Although the TREK-2 channels are distinct from voltage-activated channels (Kv family) or inward rectifier channels (Kir family), to a certain degree their kinetics resemble those of Kv and Kir channels. The probability of opening (Po) of TREK-2 channels increases with membrane depolarization [6], and the inward conductance exceeds the outward conductance (the latter does not result from asymmetrical ion concentration) [7]. In our work, we examined how the rectifying properties of the TREK-2-like channel depend on the presence of divalent ions, such as Mg 2+ and Ca 2+ , in extracellular solution in temperature close to physiological.…”

Section: Introductionmentioning

confidence: 99%

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Extracellular divalent ions modulate TREK-2-like channel conductance in prefrontal pyramidal neurons in rats

Nurowska

Adamiec

Dworakowska

2019

Medical Research Journal

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Background: The aim of the study was to investigate, with the use of the patch clamp technique, the dependence of the conductance of the TREK-2-like potassium leak channel in the medial prefrontal cortex pyramidal neurons on the presence of extracellular magnesium and calcium ions. It is suspected that TREK-2 channels regulate mood and may be associated with the pathophysiology of depression. Since magnesium and calcium deficiency contribute to depressive symptoms, we investigated how TREK-2-like channel pore properties change in the absence of divalent cations. Results: Single-channel currents were recorded in a cell-attached configuration in enzymatically dispersed pyramidal neurons of the prefrontal cortex in rats. Spontaneous TREK-2-like channel activity was recorded either in the presence or absence of magnesium and calcium ions in extracellular solution. A significant increase in the inward channel conductance was observed when divalent cations were removed from the extracellular solution. Inward rectification was also increased when the bath temperature was raised to 34-37°C. Conclusions: The study confirmed that the activity of TREK-2-like channels is affected by the presence of magnesium and calcium ions in the extracellular solution. Therefore, in vivo, the TREK-2-like channel may possibly participate in the prefrontal cortex dysfunction associated with the deficiency of divalent cations.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Extracellular divalent ions modulate TREK-2-like channel conductance in prefrontal pyramidal neurons in rats

Nurowska

Adamiec

Dworakowska

2019

Medical Research Journal

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Age‐dependent expression of Nav1.9 channels in medial prefrontal cortex pyramidal neurons in rats

Gawlak

Szulczyk

Berłowski

et al. 2017

Developmental Neurobiology

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Developmental changes that occur in the prefrontal cortex during adolescence alter behavior. These behavioral alterations likely stem from changes in prefrontal cortex neuronal activity, which may depend on the properties and expression of ion channels. Nav1.9 sodium channels conduct a Na current that is TTX resistant with a low threshold and noninactivating over time. The purpose of this study was to assess the presence of Nav1.9 channels in medial prefrontal cortex (mPFC) layer II and V pyramidal neurons in young (20-day old), late adolescent (60-day old), and adult (6- to 7-month old) rats. First, we demonstrated that layer II and V mPFC pyramidal neurons in slices obtained from young rats exhibited a TTX-resistant, low-threshold, noninactivating, and voltage-dependent Na current. The mRNA expression of the SCN11a gene (which encodes the Nav1.9 channel) in mPFC tissue was significantly higher in young rats than in late adolescent and adult rats. Nav1.9 protein was immunofluorescently labeled in mPFC cells in slices and analyzed via confocal microscopy. Nav1.9 immunolabeling was present in layer II and V mPFC pyramidal neurons and was more prominent in the neurons of young rats than in the neurons of late adolescent and adult rats. We conclude that Nav1.9 channels are expressed in layer II and V mPFC pyramidal neurons and that Nav1.9 protein expression in the mPFC pyramidal neurons of late adolescent and adult rats is lower than that in the neurons of young rats. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1371-1384, 2017.

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Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats

Shinohara

Arakaki

Amano

et al. 2020

Neuropsychopharm Rep

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Kinetic properties and adrenergic control of TREK-2-like channels in rat medial prefrontal cortex (mPFC) pyramidal neurons

Cited by 7 publications

References 80 publications

Extracellular divalent ions modulate TREK-2-like channel conductance in prefrontal pyramidal neurons in rats

Extracellular divalent ions modulate TREK-2-like channel conductance in prefrontal pyramidal neurons in rats

Age‐dependent expression of Nav1.9 channels in medial prefrontal cortex pyramidal neurons in rats

Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats

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