Kinetic performance optimisation for liquid chromatography: Principles and practice

Causon, Tim J.; Broeckhoven, Ken; Hilder, Emily F.; Shellie, Robert A.; Desmet, Gert; Eeltink, Sebastiaan

doi:10.1002/jssc.201000904

Cited by 27 publications

(12 citation statements)

References 57 publications

(60 reference statements)

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“…Fundamentally, column chemistry [ 12 , 16 , 17 ], mobile phase composition [ 12 , 16 ], and column temperature [ 18 , 19 ] are the essential factors in defining chromatographic separation during LC analyses. For instance, under reverse-phase conditions, a phenyl-derived column matrix may produce a different elution profile (i.e., number of isomers separated) compared to an alkyl-derived column matrix, due to the interactions involved in the retention mechanism of the respective columns [ 12 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Revising Reverse-Phase Chromatographic Behavior for Efficient Differentiation of Both Positional and Geometrical Isomers of Dicaffeoylquinic Acids

Masike

Dubery

Steenkamp

et al. 2018

Journal of Analytical Methods in Chemistry

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Dicaffeoylquinic acids (diCQAs) are plant metabolites and undergo trans-cis-isomerization when exposed to UV irradiation. As such, diCQAs exist in both trans-and cis-configurations and amplify the already complex plant metabolome. However, analytical differentiation of these geometrical isomers using mass spectrometry (MS) approaches has proven to be extremely challenging. Exploring the chromatographic space to develop possible conditions that would aid in differentially separating and determining the elution order of these isomers is therefore imperative. In this study, simple chromatographic parameters, such as column chemistry (phenyl versus alkyl), mobile phase composition (methanol or acetonitrile), and column temperature, were investigated to aid in the separation of diCQA geometrical isomers. e high-performance liquid chromatography photodiode array (HPLC-PDA) chromatograms revealed four isomers post UV irradiation of diCQA authentic standards. e elution profile/order was seen to vary on different reverse-phase column chemistries (phenyl versus alkyl) using different mobile phase composition. Here, the elution profile/order on the phenyl-derived column matrices (with methanol as the mobile phase composition) was observed to be relatively reproducible as compared to the alkyl (C 18 ) columns. Chromatographic resolution of diCQA geometrical isomers can be enhanced with an increase in column temperature. Lastly, the study highlights that chromatographic elution order/profile cannot be relied upon to fathom the complexity of isomeric plant metabolites.

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Section: Introductionmentioning

confidence: 99%

Revising Reverse-Phase Chromatographic Behavior for Efficient Differentiation of Both Positional and Geometrical Isomers of Dicaffeoylquinic Acids

Masike

Dubery

Steenkamp

et al. 2018

Journal of Analytical Methods in Chemistry

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“…A prediction of these aspects not explicitly covered by the van Deemter formalism is possible by investigating the kinetic plots (Causon et al 2011). …”

Section: Packed Beds For Catalytic Reactionsmentioning

confidence: 99%

Microfluidics in Planar Microchannels: Synthesis of Chemical Compounds On-Chip

Arima

Watts

Pascali

2014

Lab-on-a-Chip Devices and Micro-Total Analysis Systems

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Microreactors are a wide class of devices that are currently playing a prominent role in several research fields such as biology, medicine, food chemistry, environmental analysis, up to the production of compounds in organic chemistry. Several typologies of microreactors have been produced with tubular or planar shapes, of different materials and designs. In this chapter, an overview of planar microchannel-based microreactors and their application to organic chemistry is given. Initially, after recalling the main theoretical parameters of microfluidics, an introduction of the proposed technology and the main requirements to perform mixing, which is essential to perform chemical synthesis on-chip, is presented. Silicon and glass microreactors, the most common planar systems for organic chemistry, are described with the aim of pointing out the most important parameters to be taken into consideration in the planning of a specific microreactor to be used for mixing, purification or crystallization of chemicals at the microscale. Then, several applications of initially described microreactors to organic chemistry for research applications are given. In the next section, the use of planar microchannel microreactors in the field of radiochemistry is reported. The radiopharmaceutical application is not casual, being a sector in which the microreactor technology is very promising, due to the need of quickly producing small and fresh amounts of products in a controlled environment. Finally, for completeness, other approaches beyond planar microchannels are mentioned: mesoreactors towards industrial level synthesis and micro-vessels for radiochemistry.

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“…Furthermore, the ratio of the dwell time with respect to t 0 time needs to be kept constant, but this constraint is often omitted for reasons of practicality. The principles and practice to create gradient kinetic plots have been reviewed by Causon et al [40].…”

Section: Kinetic Performance Limitsmentioning

confidence: 99%

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Dores-Sousa

Vos

Eeltink

2018

J of Separation Science

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This review describes chromatographic dispersion and different plate‐height models frequently used to assess the chromatographic performance of ultra‐high‐pressure liquid chromatography column technology. Furthermore, different performance indices, including the resolution, the separation impedance, and kinetic plots are discussed allowing to quantify and visualize the resolving power in liquid chromatography. The construction of kinetic plots is explained, and different visualization approaches are highlighted. Finally, key instrument and column‐technology developments to advance the kinetic performance limits are discussed and selected state‐of‐the‐art applications are highlighted.

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Kinetic performance optimisation for liquid chromatography: Principles and practice

Cited by 27 publications

References 57 publications

Revising Reverse-Phase Chromatographic Behavior for Efficient Differentiation of Both Positional and Geometrical Isomers of Dicaffeoylquinic Acids

Revising Reverse-Phase Chromatographic Behavior for Efficient Differentiation of Both Positional and Geometrical Isomers of Dicaffeoylquinic Acids

Microfluidics in Planar Microchannels: Synthesis of Chemical Compounds On-Chip

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

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