Kinetic parameters of Aspergillus awamori in submerged cultivations on whole wheat flour under oxygen limiting conditions

Koutinas, Apostolis; Wang, R.; Kookos, Ioannis K.; Webb, Colin

doi:10.1016/s1369-703x(03)00018-4

Cited by 26 publications

(16 citation statements)

References 38 publications

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“…Wheat flour hydrolysates produced by this method would be rich primarily in glucose but would also contain other nutrients, such as amino acids, vitamins, phosphorus and other minerals. In previous publications, it has been shown that wheat flour can support the growth of A. awamori but limited data on enzyme production has been presented so far (Koutinas et al, 2003a. This publication provides data on enzyme production during fermentation and enzyme activity requirements for wheat flour hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

“…The supernatant from a 10 mL fermentation sample was separated by centrifugation at 3,000g for 10 min and was utilised for the analysis of glucose and enzyme activities. The solids were used to measure the remaining starch that had not been hydrolysed in the fermentation broth (Koutinas et al, 2003a). An Analox GL6 glucose analyser was used to measure glucose concentration.…”

Section: Methodsmentioning

confidence: 99%

“…Three parameters (K S , K O and K 2 ) are fixed to values calculated in previous studies to avoid problems related to their identifiability. The remaining 11 parameters are estimated based on a non-linear, weighted least squares mathematical programming problem (Koutinas et al, 2003a) which was solved as an optimal control problem using simulated annealing (Kookos, 2004), a stochastic global optimisation algorithm, implemented in MATLAB. To facilitate solution of the optimisation problem and to avoid potential unrealistic optima all parameters were bound constrained.…”

Section: Model Descriptionmentioning

confidence: 99%

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Cereal‐based biorefinery development: Integrated enzyme production for cereal flour hydrolysis

Koutinas

Arifeen

Wang

et al. 2006

Biotech & Bioengineering

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Restructuring the traditional fermentation industry into viable biorefineries for the production of fuels, chemicals and plastics is essential in order to replace (petro)chemical processing. This work presents engineering aspects of Aspergillus awamori submerged fermentation for on-site production of an enzymatic consortium that contains glucoamylase, protease and phosphatase. The crude broth filtrate was used for the production of wheat flour hydrolysates. Improvements on traditional starch hydrolysis carried out in two stages (liquefaction and saccharification) were attempted through integration of unit operations and reduction of processing temperature and reaction duration. An initial increase of temperature to 68 degrees C and a subsequent decrease to 60 degrees C for the rest of the enzymatic hydrolysis resulted in a starch to glucose conversion yield of 94 and 92% when a wheat flour concentration and commercial starch concentration of 225 g L(-1) was used, respectively. The use of crude broth filtrates resulted in the simultaneous hydrolysis of wheat protein and phytic acid, as was indicated by the increase in free amino nitrogen and phosphorus concentration, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Model Descriptionmentioning

confidence: 99%

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Cereal‐based biorefinery development: Integrated enzyme production for cereal flour hydrolysis

Koutinas

Arifeen

Wang

et al. 2006

Biotech & Bioengineering

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“…The best studied Aspergillus endoxylanases, with different substrate specificity, are the three enzymes from Aspergillus awamori. The fungal strain A. awamori is well known for its frequent use in the food industry for the production of xylanase enzymes (5,4). Many plants produce starch, a high molecular weight polymer of glucose.…”

Section: Introductionmentioning

confidence: 99%

“…The starch is used by many microorganisms as storage of carbon and energy source. The starch molecule consists of two types of glucan polymers: highly branched amylopectin and linear amylase (5).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of α-Amylase and Endoxylanase, Produced byAspergillusMutant Strains

Evstatieva¹,

Nikolova²,

Ilieva³

et al. 2010

Biotechnology & Biotechnological Equipment

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Consolidated bioprocessing of starchy substrates into ethanol by industrial Saccharomyces cerevisiae strains secreting fungal amylases

Favaro

Viktor

Rose

et al. 2015

Biotech & Bioengineering

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The development of a yeast strain that converts raw starch to ethanol in one step (called Consolidated Bioprocessing, CBP) could significantly reduce the commercial costs of starch-based bioethanol. An efficient amylolytic Saccharomyces cerevisiae strain suitable for industrial bioethanol production was developed in this study. Codon-optimized variants of the Thermomyces lanuginosus glucoamylase (TLG1) and Saccharomycopsis fibuligera α-amylase (SFA1) genes were δ-integrated into two S. cerevisiae yeast with promising industrial traits, i.e., strains M2n and MEL2. The recombinant M2n[TLG1-SFA1] and MEL2[TLG1-SFA1] yeast displayed high enzyme activities on soluble and raw starch (up to 8118 and 4461 nkat/g dry cell weight, respectively) and produced about 64 g/L ethanol from 200 g/L raw corn starch in a bioreactor, corresponding to 55% of the theoretical maximum ethanol yield (g of ethanol/g of available glucose equivalent). Their starch-to-ethanol conversion efficiencies were even higher on natural sorghum and triticale substrates (62 and 73% of the theoretical yield, respectively). This is the first report of direct ethanol production from natural starchy substrates (without any pre-treatment or commercial enzyme addition) using industrial yeast strains co-secreting both a glucoamylase and α-amylase.

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Kinetic parameters of Aspergillus awamori in submerged cultivations on whole wheat flour under oxygen limiting conditions

Cited by 26 publications

References 38 publications

Cereal‐based biorefinery development: Integrated enzyme production for cereal flour hydrolysis

Cereal‐based biorefinery development: Integrated enzyme production for cereal flour hydrolysis

Identification and Characterization of α-Amylase and Endoxylanase, Produced byAspergillusMutant Strains

Consolidated bioprocessing of starchy substrates into ethanol by industrial Saccharomyces cerevisiae strains secreting fungal amylases

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