Kinetic modeling, production and characterization of an acidic lipase produced by Enterococcus durans NCIM5427 from fish waste

Abstract

Enterococcus durans NCIM5427 (ED-27), capable of producing an intracellular acid stable lipase, was isolated from fish processing waste. Its growth and subsequent lipase production was optimized by Box Behneken design (optimized conditions: 5 % v/v fish waste oil (FWO), 0.10 mg/ml fish waste protein hydrolysates (FWPH) at 48 h of fermentation time). Under optimized conditions, ED-27 showed a 3.0 fold increase (207.6 U/ml to 612.53 U/ml) in lipase production, as compared to un-optimized conditions. Cell growth and lipase production was modeled using Logistic and LuedekingPiret model, respectively; and lipase production by ED-27 was found to be growth-associated. Lipase produced by ED-27 showed stability at low pH ranges from 2 to 5 with its optimal activity at 30°C , pH 4.6; showed metal ion dependent activity wherein its catalytic activity was activated by barium, sodium, lithium and potassium (10 mM); reduced by calcium and magnesium (10 mM). However, iron and mercury (5 mM) completely inactivated the enzyme. In addition, modifying agents like SDS, DTT, β-ME (1%v/v) increased activity of lipase of ED-27; while, PMSF, DEPC and ascorbic acid resulted in a marked decrease. ED-27 had maximum cell growth of 9.90309 log CFU/ml under optimized conditions as compared to 13 log CFU/ml in MRS. The lipase produced has potential application in poultry and slaughterhouse waste management.

Impacts of different concentrations of Copper and Zinc on in vitro responses of Azotobacter chroococum in biomass and nitrogen fixing outputs

“…A linear regression plot of nitrogen fixation rate against specific growth rate generated α = 0.0010156 and β = 0.14032 for copper amended trials (Figure 3). Since, α > 0 and β > 0, then it implies that in this trial, nitrogen fixation was associated with both biomass growth and biomass concentration of A. chroococum (Ramakrishnan et al, 2015). However, since β (0.14032) is far greater than α (0.0010156), and also since the value of α = 0.0010156 is not significant at 0.01 level of significance, one may infer that nitrogen fixation yields in these trials were more significantly influenced by the non-growth associated constant (β), or otherwise insignificantly determined by the growth associated constant, α (Ahmad et al, 2011;Thierie, 2013).…”

Expression of novel acidic lipase from Micrococcus luteus in Pichia pastoris and its application in transesterification

“…Notwithstanding the many lipase-producing strains that have been reported, the investigation on acidic lipase-producing strain is still a minority. Several acidic lipases have been studied to have optimum activity or stability under acidic conditions and have been isolated from Aspergillus niger NCIM 1207 [ 15 ], Penicillium simplicissimum [ 16 ], A. niger AN0512 [ 17 ], A. niger ANL [ 18 , 19 ], Pseudomonas gessardii [ 14 ], P. fluorescens [ 20 ], A. terreus [ 21 ], Enterococcus durans NCIM5427 [ 22 ], Candida viswanathii [ 23 ], Bacillus pumilus [ 24 ], B. subtilis [ 25 ], Neosartorya fischeri P1 [ 26 ], Meyerozyma guilliermondii [ 13 ], and Rasamsonia emersonii [ 27 ].…”

Utilization of fish meal and fish oil for production of Cryptococcus sp. MTCC 5455 lipase and hydrolysis of polyurethane thereof

“…Fish protein hydrolysate has been used as one of the ingredients in the media for the growth of bacteria such as Bacillus subtilis (Ellouz et al 2001), Enterococcus durans (Ramakrishnan et al 2013) and Bacillus cereus BG1 (Sellami-Kamoun et al 2011) and fungi, including Rhizopus oryzae (Ghorbel et al 2005) and Aspergillus oryzae (GarciaGomez et al 2009). However, the reports on production of enzymes using fish meal, as a source of protein (Awan et al 2010) and fish oil, as inducers (Ikemoto and Ota 1996;Kamini et al 2000) are very much limited.…”