Kinetic modeling of lutein production by heterotrophic Chlorella at various pH and temperatures

Shi, Xianming; Wu, Zhengyun; Chen, Feng

doi:10.1002/mnfr.200600037

Cited by 65 publications

(28 citation statements)

References 32 publications

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“…However the heterotrophic growth was seriously inhibited when the initial pH value was too high to 10.0 or too low to 4.0 (p < 0.05). Results from Shi et al (2006), Jiang and Chen (2000) also suggested that the pH values of 6.0 and 7.2, a closely neutral condition of medium, were optimal for heterotrophic culture of C. protothecoides and C. cohnii respectively in order to obtain the highest biomass. A slight rise of lipid content when initial pH of medium was increased from 7.9 to 10.0 is reasonably contributed to the alkaline stress.…”

Section: Effect Of Ph and Temperature Influence On T Minus Heterotrophymentioning

confidence: 91%

Heterotrophy of filamentous oleaginous microalgae Tribonema minus for potential production of lipid and palmitoleic acid

Zhou

Wang

Chen

et al. 2017

Bioresource Technology

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h i g h l i g h t sHeterotrophic ability of microalgae Tribonema minus was identified for the first time. Glucose and urea were the optimal sources for heterotrophic fermentation of T. minus. Heterotrophic fermentation and high valuable co-product producing are thought to be effective ways to improve the economic viability and feasibility of commercial production of microalgae biofuels. This work reported the heterotrophic cultivation of Tribonema minus for lipid and palmitoleic acid (a novel functional fatty acid) production. Firstly, the heterotrophic ability of T. minus was identified for the first time with significant promotion in biomass and lipid productivity, and glucose and urea were then selected as the optimal carbon and nitrogen sources. Moreover, nutrient concentrations and culture conditions were optimized. Highest biomass and lipid productivity of 30.8 g L À1 and 730 mg L À1 d À1 were obtained respectively by adding 80 g L À1 glucose at once. In addition, 2 g L À1 urea, 0.8 g L À1 K 2 HPO 4 , 24 mg L À1 ammonium ferric citrate, initial pH of 6, and temperature of 27°C were determined as the appropriate conditions for heterotrophic growth and lipid production.

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Section: Effect Of Ph and Temperature Influence On T Minus Heterotrophymentioning

confidence: 91%

Heterotrophy of filamentous oleaginous microalgae Tribonema minus for potential production of lipid and palmitoleic acid

Zhou

Wang

Chen

et al. 2017

Bioresource Technology

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“…Crypthecodinium cohnii can directly activate acetate to acetyl-CoA, the basic building block of fatty acids synthesis [48]. Also, it has been remarked that the heterotrophic growth of C. protothecoides supplied with acetate, glucose, or other organic compounds as carbon source results in high biomass and high content of lipid in cells [52]. To improve acetate or any other carbon source utilization, actively dividing microalgal cells from the exponential phase should be used as inoculums [2,41].…”

Section: Effect Of Acetate As Carbon Source With Ph Adjustmentmentioning

confidence: 99%

Oil Accumulation via Heterotrophic/Mixotrophic Chlorella protothecoides

Heredia-Arroyo

Wei

2010

Appl Biochem Biotechnol

211

107

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Microalgal oil is a potential energy source because it can be easily converted to fatty acid methyl ester or hydrocarbon type of diesel, and it is produced with relatively higher productivity compared with oil from plants and animals. Heterotrophic growth of microalgae is superior due to its high final product concentration; however, the cost of the raw materials is unacceptable if sugar is utilized as the carbon source. The aim of this study is to optimize the lipid accumulation of Chlorella protothecoides by using carbon sources other than glucose in heterotrophic and mixotrophic cultures. Different factors such as different carbon sources, carbon to nitrogen ratio, initial pH level, salinity, and rotational speed are studied in affecting the cell growth and the oil accumulation. Our experiments revealed that the heterotrophic and mixotrophic cultures of C. protothecoides grew better than autotrophic cultures. C. protothecoides can grow on glycerol or acetate, as well as on glucose. Several stress factors were confirmed or discovered to significantly increase the lipid content of microalgae cells. The replacement of glycerol and acetate as carbon sources for microalgae cultivations provides potential for waste utilization: glycerol from biodiesel industry and acetate from biohydrogen production.

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“…Recently, significant attention has been drawn to several alternative techniques for mass cultivation of algae, including heterotrophic and mixotrophic processes (Chen and Zhang 1997). Much work has been done on heterotrophic and mixotrophic growth of the green algae Chlorella (Shi et al , 2006Xu et al 2006) and Haematococcus (Kang et al 2005;Kaewpintong et al 2007). These algae can use different organic carbon sources such as glucose and acetate.…”

Section: Introductionmentioning

confidence: 99%

Growth characteristics of the cyanobacterium Nostoc flagelliforme in photoautotrophic, mixotrophic and heterotrophic cultivation

2008

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Nostoc flagelliforme is a terrestrial cyanobacterium with high economic value. Dissociated cells separated from a natural colony of N. flagelliforme were cultivated for 7 days under either phototrophic, mixotrophic or heterotrophic culture conditions. The highest biomass, 1.67 g L −1 cell concentration, was obtained under mixotrophic culture, representing 4.98 and 2.28 times the biomass obtained in phototrophic and heterotrophic cultures, respectively. The biomass in mixotrophic culture was not the sum as that in photoautotrophic and heterotrophic cultures. During the first 4 days of culture, the cell concentration in mixotrophic culture was lower than the sum of those in photoautotrophic and heterotrophic cultures. However, from the 5th day, the cell concentration in mixotrophic culture surpassed the sum of those obtained from the other two trophic modes. Although the inhibitor of photosynthetic electron transport DCMU [3-(3,4-dichlorophenyl)-1,1-dimethylurea] efficiently inhibited autotrophic growth of N. flagelliforme cells, under mixotrophic culture they could grow by using glucose. The addition of glucose changed the response of N.flagelliforme cells to light. The maximal photosynthetic rate, dark respiration rate and light compensation point in mixotrophic culture were higher than those in photoautotrophic cultures. These results suggest that photoautotrophic (photosynthesis) and heterotrophic (oxidative metabolism of glucose) growth interact in mixotrophic growth of N. flagelliforme cells.

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Kinetic modeling of lutein production by heterotrophic Chlorella at various pH and temperatures

Cited by 65 publications

References 32 publications

Heterotrophy of filamentous oleaginous microalgae Tribonema minus for potential production of lipid and palmitoleic acid

Heterotrophy of filamentous oleaginous microalgae Tribonema minus for potential production of lipid and palmitoleic acid

Oil Accumulation via Heterotrophic/Mixotrophic Chlorella protothecoides

Growth characteristics of the cyanobacterium Nostoc flagelliforme in photoautotrophic, mixotrophic and heterotrophic cultivation

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