Kinetic Modeling of Dihydroxyacetone Production from Glycerol by Gluconobacter oxydans ATCC 621 Resting Cells: Effect of Fluid Dynamics Conditions

Morena, Susana de la; Wojtusik, Mateusz; Santos, Victoria E.; Garcı́a-Ochoa, Félix

doi:10.3390/catal10010101

Cited by 11 publications

(5 citation statements)

References 31 publications

(58 reference statements)

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“…For improved DHA production, different Gluconobacter sp. strains have been studied and included Gluconobacter frateurii , Gluconobacter thailandicus, and G. oxydans (de la Morena et al 2020 ; Jittjang et al 2020 ; Poljungreed & Boonyarattanakalin, 2017 ). To identify optimal DHA production, five wild-type industrial strains, G. oxydans WSH-004, G. oxydans WSH-003, G. oxydans 621H, G. japonicus CGMCC 1.49, and G. cerinus CGMCC 1.110 were selected and compared (Liu et al 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Efficient 1,3-dihydroxyacetone biosynthesis in Gluconobacter oxydans using metabolic engineering and a fed-batch strategy

Zeng

Shan

Liu

et al. 2022

Bioresour. Bioprocess.

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Abstract1,3-Dihydroxyacetone (DHA) is a commercially important chemical and widely used in cosmetics, pharmaceuticals, and food industries as it prevents excessive water evaporation, and provides anti-ultraviolet radiation protection and antioxidant activity. Currently, the industrial production of DHA is based on a biotechnological synthetic route using Gluconobacter oxydans. However, achieving higher production requires more improvements in the synthetic process. In this study, we compared DHA synthesis levels in five industrial wild-type Gluconobacter strains, after which the G. oxydans WSH-003 strain was selected. Then, 16 dehydrogenase genes, unrelated to DHA synthesis, were individually knocked out, with one strain significantly enhancing DHA production, reaching 89.49 g L−1 and 42.27% higher than the wild-type strain. By optimizing the culture media, including seed culture and fermentation media, DHA production was further enhanced. Finally, using an established fed-batch fermentation system, DHA production reached 198.81 g L−1 in a 5 L bioreactor, with a glycerol conversion rate of 82.84%. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Efficient 1,3-dihydroxyacetone biosynthesis in Gluconobacter oxydans using metabolic engineering and a fed-batch strategy

Zeng

Shan

Liu

et al. 2022

Bioresour. Bioprocess.

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“…In this way, the metabolic pathways are active towards SA production and cellular maintenance, but there is no bacterial growth. Concurrently, the number of by-products is also reduced dramatically [18][19][20], this being one of the main advantages associated with this type of operation. In fact, Escanciano et al (2022) [17] managed to reduce the quantity of by-products generated by 27.5% in the production of SA from xylose.…”

Section: Introductionmentioning

confidence: 99%

“…Separating growth and production into two different steps allows for a better optimisation of each step and of the overall process. Considering the scale-up, the proposed bioprocess would lead to higher productivity linked to the reduction of dead times as well as to avoid substrate inhibition that was observed when using growing cells as biocatalyst [18,20,21].…”

Section: Introductionmentioning

confidence: 99%

Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

et al. 2023

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Succinic acid (SA) is one of the most prominent C4 biomass-based platform chemicals that can be biologically obtained. This article verifies, for the first time, the possibility of producing succinic acid with fed-batch or repeated batch operations with Actinobacillus succinogenes in a resting state, that is, in the absence of a nitrogen source. In this work it is possible to optimise separately the stages of cell growth and production in the fed-batch or repeated batch modes, minimising the costs associated with the nitrogen source and facilitating the subsequent purification of SA. These experiments were carried out with xylose, the most abundant monosaccharide in hemicelluloses, with the results subsequently being compared to those obtained in equivalent operations carried out with cells in a state of growth. First, a cost-effective synthetic growth medium was proposed and successfully employed for SA production. Biocatalysts’ reutilisation showed that the bioprocess can be carried out successfully in repeated batch and fed-batch modes. The best mode for growing cells is repeated batch, achieving a maximum productivity of 0.77 g‧L−1‧h−1, a selectivity of 53% and a yield of 51% with respect to xylose consumed. In contrast, the fed-batch mode was found to be the most convenient mode with resting cell biocatalyst, reaching a maximum productivity of 0.83 g‧L−1‧h−1, a selectivity of 0.78 g‧g−1 and a yield of 68% with respect to the xylose consumed. In addition, by-product formation is significantly reduced when employing resting cells. An unstructured non-segregated kinetic model was developed for both biocatalysts, capable of simulating cell growth, xylose consumption, SA production and by-product generation, with successful estimation of kinetic parameters supported by statistical criteria.

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“…The use of this conductive composite in combination with G. oxydans cells in an MFC can be appropriate for creating a device capable of long-term low-pH wastewater treatment and simultaneous efficient electric energy generation. Moreover, these microorganisms are used in food [ 30 , 31 ], pharmaceutical [ 32 , 33 ] and cosmetic [ 34 ] industries, so they can be also used in wastewater treatment systems of these biotechnological enterprises.…”

Section: Introductionmentioning

confidence: 99%

Gluconobacter Oxydans-Based MFC with PEDOT:PSS/Graphene/Nafion Bioanode for Wastewater Treatment

et al. 2022

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Microbial fuel cells (MFCs) are a variety of bioelectrocatalytic devices that utilize the metabolism of microorganisms to generate electric energy from organic matter. This study investigates the possibility of using a novel PEDOT:PSS/graphene/Nafion composite in combination with acetic acid bacteria Gluconobacter oxydans to create a pure culture MFC capable of effective municipal wastewater treatment. The developed MFC was shown to maintain its activity for at least three weeks. The level of COD in municipal wastewater treatment was reduced by 32%; the generated power was up to 81 mW/m2 with a Coulomb efficiency of 40%. Combining the MFC with a DC/DC boost converter increased the voltage generated by two series-connected MFCs from 0.55 mV to 3.2 V. A maximum efficiency was achieved on day 8 of MFC operation and was maintained for a week; capacitors of 6800 µF capacity were fully charged in ~7 min. Thus, G. oxydans cells can become an important part of microbial consortia in MFCs used for treatment of wastewaters with reduced pH.

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Kinetic Modeling of Dihydroxyacetone Production from Glycerol by Gluconobacter oxydans ATCC 621 Resting Cells: Effect of Fluid Dynamics Conditions

Cited by 11 publications

References 31 publications

Efficient 1,3-dihydroxyacetone biosynthesis in Gluconobacter oxydans using metabolic engineering and a fed-batch strategy

Efficient 1,3-dihydroxyacetone biosynthesis in Gluconobacter oxydans using metabolic engineering and a fed-batch strategy

Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

Gluconobacter Oxydans-Based MFC with PEDOT:PSS/Graphene/Nafion Bioanode for Wastewater Treatment

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