Kinetic model for lactose hidrolysis in a recirculation hollow-fibre bioreactor

Jurado, E.; Camacho, F.; Luzón, G.; Vicaria, José M.

doi:10.1016/j.ces.2003.09.035

Cited by 22 publications

(21 citation statements)

References 40 publications

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“…Their application to reduce the lactose content in milk products is widely known (JURADO et al, 2004). More recently, commercial preparations capable of acting on a wide range of pH have been available and hold great potential for use in lactic fermentation because hydrolytic activity remains throughout acidification process.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content

et al. 2012

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In a single-stage process with the simultaneous addition of β -galactosidase and lactic culture, the lactose conversion, the processing time, viscosity and syneresis were evaluated. Fermentation was promoted by lactic culture containing two probiotic microorganisms, Bifidobacterium animalis and Lactobacillus acidophilus, associated with the typical microorganisms of yogurt.An enzymatic preparation containing β -galactosidases from Kluyveromyces lactis and Aspergillus niger was used. , enzyme concentration of 0.5 g L -1 and enzyme addition at the beginning of fermentation. In these conditions, a decrease in processing time (from 4.55 to 3.68 h) and an increase in lactose conversion (from 15.2 to 97.9%) were observed in relation to the fermentation without enzyme addition, and no detrimental changes in some physical properties of yogurt (viscosity, and syneresis).Index terms: Enzymatic catalysis, lactose hydrolysis, yogurt physical properties, β -galactosidase. RESUMONo processo em um único estágio com a adição simultânea de β -galactosidase e de cultura lática, foram avaliadas a conversão da lactose, o tempo de processamento, a viscosidade e a sinérese. A fermentação foi promovida por cultura láctica, contendo dois micro-organismos probióticos, Bifidobacterium animalis e Lactobacillus acidophilus, associados aos micro-organismos característicos do iogurte. Foi utilizado um preparado enzimático contendo β -galactosidases obtidas de Kluyveromyces lactis e de Aspergillus niger.Os efeitos da concentração inicial de lactose, concentração de enzima e tempo de adição da enzima foram avaliados por meio de um planejamento experimental (2 3 ensaios mais três pontos centrais). As seguintes condições foram estabelecidas: concentração inicial de lactose de 91 g L -1, concentração de enzima de 0.5 g L -1 e adição da enzima no início da fermentação. Nessas condições, um decréscimo no tempo de processamento (de 4,55 h para 3,68 h) e um incremento na conversão de lactose (de 15,2% para 97,9%) foram observados em relação à fermentação sem a adição da enzima, sem alterações prejudiciais em algumas propriedades físicas do iogurte (viscosidade, e sinérese). Termos para indexação:Catálise enzimática, hidrólise da lactose, propriedades físicas do iogurte, β -galactosidase.

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Section: Introductionmentioning

confidence: 99%

Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content

et al. 2012

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“…Others function at acidic pH (5.5-4.5) and are obtained from moulds of the genus Aspergillus. Their application with the objective of reducing the lactose content of milk products is widely known (ABU-REESH, 2000;JURADO et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Lactose conversion and the synthesis of galactooligosaccharides in a simultaneous lagged bioprocess using β-galactosidases and probiotic microorganisms

Martins¹,

Manera²,

Monteiro³

et al. 2011

BJFT

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SummaryLactose conversion and the production of galactooligosaccharides (GOS) were evaluated in a simultaneous bioprocess using probiotic microorganisms and the addition of β-galactosidases, lagged or otherwise. The fermentation was carried out using normal yogurt cultures, Streptococcus thermophilus and Lactobacillus bulgaricus, associated with two probiotic microorganisms, Bifidobacterium animalis and Lactobacillus acidophilus. An enzymatic preparation containing β-galactosidases of distinct origins, obtained from Kluyveromyces lactis and Aspergillus niger was used. The simultaneous bioprocess was evaluated, varying the initial lactose and enzyme concentrations and also the time of addition of the enzymes, with a lag in the enzyme application in relation to the beginning of fermentation. The results indicated a positive impact on lactose conversion, reducing the disaccharide concentration by between 97.7 and 99.7%, as compared to 13.8 and 19.8% in fermentations without enzyme addition, obtaining between 2.7 and 4.2 g.L -1 GOS in the lagged processes carried out with the highest initial lactose concentration and the addition of the lowest enzyme concentration. Key words: Enzyme catalysis; Lactose conversion; Lactic fermentation; GOS. ResumoEm bioprocesso simultâneo conduzido por microrganismos probióticos e através da adição, defasada ou não, de β-galactosidases, foram avaliadas a redução da lactose e a obtenção de galacto-oligossacarídeos (GOS). A fermentação foi conduzida por culturas características de iogurte, Lactobacillus bulgaricus e Streptococcus thermophilus, associadas a dois microrganismos probióticos, Bifidobacterium animalis e Lactobacillus acidophilus. Foi utilizado um preparado enzimático contendo β-galactosidases de origens distintas, obtidas de Kluyveromyces lactis e de Aspergillus niger. O bioprocesso simultâneo foi avaliado variando as concentrações iniciais de lactose e de enzima, e o tempo de adição da enzima, caracterizando uma defasagem na aplicação da enzima em relação ao início da fermentação. Os resultados indicaram um impacto positivo na conversão da lactose, reduzindo entre 97,7 e 99,7% a concentração desse dissacarídeo, em comparação com 13,8 e 19,8% nas fermentações sem adição de enzima, bem como a obtenção de GOS entre 2,7 e 4,2 g.L -1 nos processos defasados realizados com concentração inicial máxima de lactose e com concentração mínima de enzima adicionada. Palavras-chave: Catálise enzimática; Conversão da lactose; Fermentação lática; GOS.Lactose conversion and the synthesis of galactooligosaccharides in a simultaneous lagged bioprocess using β-galactosidases and probiotic microorganisms Conversão da lactose e síntese de galacto-oligossacarídeos em bioprocesso simultâneo defasado com β-galactosidases e microrganismos probióticos Autores | Authors

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“…Jurado et al [13,14] studied different enzymatic reactions in a hollow-fibre reactor. Under the experimental conditions assayed by these authors, there was no appreciable resistance to the transference of substrates and products by the membrane.…”

Section: Introductionmentioning

confidence: 99%

“…The set-up consists of a jacketed-stirred tank (0.250 L), two peristaltic pumps, a rotameter and a hollow-fibre module submerged in a thermostat-controlled bath [13,14]. The module has two compartments, one being the fibre lumens (called the lumen circuit) and the other being the space between fibres (called extracapillary space or ECS) where the enzyme is confined.…”

Section: Introductionmentioning

confidence: 99%

Kinetic and enzymatic adsorption model in a recirculation hollow-fibre bioreactor

Jurado

Camacho

Luzón

et al. 2005

Bioprocess Biosyst Eng

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A general procedure has been developed to model the behaviour of enzymatic reactions in a membrane bioreactor. This procedure unifies the kinetics of the reaction and the adsorption of the enzyme or enzymatic complexes on the membrane, enabling the selection of the most appropriate kinetic model. The general procedure proposed has been particularized and applied to experimental results obtained with two enzymatic reactions carried out in a hollow-fibre reactor, enzymatic hydrolysis of lactose by beta-galactosidase and glucose-fructose isomerization by glucose isomerase. The application of the general model has allowed us to determine the mechanism of the reaction for both kinetic reactions, assuming the adsorption of the enzymatic complex EGa for lactose hydrolysis and the adsorption of the free enzyme onto the membrane for glucose-fructose isomerization.

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Kinetic model for lactose hidrolysis in a recirculation hollow-fibre bioreactor

Cited by 22 publications

References 40 publications

Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content

Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content

Lactose conversion and the synthesis of galactooligosaccharides in a simultaneous lagged bioprocess using β-galactosidases and probiotic microorganisms

Kinetic and enzymatic adsorption model in a recirculation hollow-fibre bioreactor

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