In this paper, Kernel Density Estimation (KDE) as a non-parametric estimation method is used to investigate statistical properties of nuclear spectra. The deviation to regular or chaotic dynamics, is exhibited by closer distances to Poisson or Wigner limits respectively which evaluated by Kullback-Leibler Divergence (KLD) measure. Spectral statistics of different sequences prepared by nuclei corresponds to three dynamical symmetry limits of Interaction Boson Model(IBM), oblate and prolate nuclei and also the pairing effect on nuclear level statistics are analyzed (with pure experimental data). KD-based estimated density function, confirm previous predictions with minimum uncertainty (evaluated with Integrate Absolute Error (IAE)) in compare to Maximum Likelihood (ML)-based method. Also, the increasing of regularity degrees of spectra due to pairing effect is reveal.