Kinetic detection method for thin-layer chromatography

Corcoran, Carolyn A.; Rutan, Sarah C.

doi:10.1016/s0003-2670(00)86568-x

Cited by 11 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 1 5 1 7 , 6 7 The Kalman filter has also been used to fit enzyme deactivation kinetic data,@ and kinetic data measured on thin-layer chromatographic plates. 68 …”

Section: Kinetic Analysismentioning

confidence: 99%

Recursive parameter estimation

Rutan

1990

Journal of Chemometrics

Self Cite

View full text Add to dashboard Cite

SUMMARYThe use of recursive filtering techniques for parameter estimation in a variety of areas is reviewed. In particular, the Kalman filter algorithm is described, along with several variations, including square-root, UDUT and information filters. The solution to parameter estimation problems is discussed for both linear and non-linear models. Applications described include calibration, curve resolution in spectroscopy, chromatography, electrochemistry, kinetic analysis and process monitoring.

show abstract

“…1 1 5 1 7 , 6 7 The Kalman filter has also been used to fit enzyme deactivation kinetic data,@ and kinetic data measured on thin-layer chromatographic plates. 68 …”

Section: Kinetic Analysismentioning

confidence: 99%

Recursive parameter estimation

Rutan

1990

Journal of Chemometrics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The activity of each isozyme can be calculated from the product of AP and k¿. The feasibility of using linear and nonlinear least-squares curve-fitting methods to process kinetic data that obey this type of model has been demonstrated in recent publications (12,(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Guanidinium-induced differential kinetic denaturation of alkaline phosphatase isozymes

Lewis¹,

Rutan²

1991

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

This paper examines the solution kinetics of bovine intestinal and liver alkaline phosphatase (ALP) isozymes. 4-Methylumbelliferyl phosphate is used as the substrate to study the differential kinetic behavior of ALP isozymes in the presence of guanidinium hydrochloride, a denaturant. The recursive Kalman filter algorithm for parameter estimation is used for analysis of the resulting kinetic data. A two-component first-order kinetic model with a zero-order component is used to successfully quantify intestinal and liver isozymes in synthetic mixtures. This work serves as a basis for the development of an electrophoresis separation method for ALP isozyme quantification with differential kinetic detection.

show abstract