Kinetic characterization and molecular modeling of trehalose-6-phosphate phosphatase from Anopheles gambiae and expressed in Pichia pastoris

Pessoa, Marcos CÃ©zar Fernandes; Izumi, Ã‰rika; Zanotto, Sandra PatrÃ­cia; Astolfi-Filho, Spártaco

doi:10.5897/ajb2017.16021

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…The K M values of BmiTPPC1 and BmiTPPC2 were close to those of reported TPPs (Fig. 6K, L) (Miao et al ., 2016; Shan et al ., 2016; Pessoa et al ., 2017; Liu et al ., 2017a). Interestingly, in spite of the dominance of BmiTPPB transcript in most of the samples, the K M values of BmiTPPB were an order of magnitude greater than BmiTPPC1 and BmiTPPC2 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Trehalose‐6‐phosphate phosphatases are involved in trehalose synthesis and metamorphosis in Bactrocera minax

et al. 2022

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Trehalose is the principal sugar circulating in the hemolymph of insects, and trehalose synthesis is catalyzed by trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Insect TPS is a fused enzyme containing both TPS domain and TPP domain. Thus, many insects do not possess TPP genes as TPSs have replaced the function of TPPs. However, TPPs are widely distributed across the dipteran insects, while the roles they play remain largely unknown. In this study, 3 TPP genes from notorious dipteran pest Bactrocera minax (BmiTPPB, BmiTPPC1, and BmiTPPC2) were identified and characterized. The different temporal-spatial expression patterns of 3 BmiTPPs implied that they exert different functions in B. minax. Recombinant BmiTPPs were heterologously expressed in yeast cells, and all purified proteins exhibited enzymatic activities, despite the remarkable disparity in performance between BmiTPPB and BmiTPPCs. RNA interference revealed that all BmiTPPs were successfully downregulated after double-stranded RNA injection, leading to decreased trehalose content and increased glucose content. Also, suppression of BmiTPPs significantly affected expression of downstream genes and increased the mortality and malformation rate. Collectively, these results indicated that all 3 BmiTPPs in B. minax are involved in trehalose synthesis and metamorphosis. Thus, these genes could be evaluated as insecticidal targets for managing B. minax, and even for other dipteran pests.

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Section: Discussionmentioning

confidence: 99%

Trehalose‐6‐phosphate phosphatases are involved in trehalose synthesis and metamorphosis in Bactrocera minax

et al. 2022

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“…[6,[9][10] TPP has been studied in a few insects like Delia antiqua, Anopheles gambie, Bactrocera minax, and Sitodiplosis mosellana. [9,6,[11][12] Three TPP genes from Bactrocera minax express highly in the last instar larvae or early pupal stages. [6] The expression and activity of TPP are elevated in the pre-diapause and diapause stages of Sitodiplosis mosellana.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Trehalose Synthesis in Lepidoptera Reduces Larval Fitness

Tellis,

Mohite,

Nair

et al. 2023

Advanced Biology

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Trehalose is synthesized in insects through the trehalose 6‐phosphate synthase and phosphatase (TPS/TPP) pathway. TPP dephosphorylates trehalose 6‐phosphate to release trehalose. Trehalose is involved in metamorphosis, but its relation with body weight, size, and developmental timing is unexplored. The expression and activity of TPS/TPP fluctuate depending on trehalose demand. Thus, TPS/TPP inhibition can highlight the significance of trehalose in insect physiology. TPS/TPP transcript levels are elevated in the pre‐pupal and pupal stages in Helicoverpa armigera. The inhibition of recombinantly expressed TPP by N‐(phenylthio)phthalimide (NPP), is validated by in vitro assays. In vivo inhibition of trehalose synthesis reduces larval weight and size, hampers metamorphosis, and reduces its overall fitness. Insufficient trehalose leads to a shift in glucose flux, reduced energy, and dysregulated fatty acid oxidation. Metabolomics reaffirms the depletion of trehalose, glucose, glucose 6‐phosphate, and suppressed tricarboxylic acid cycle. Reduced trehalose hampers the energy level affecting larval vitality. Through trehalose synthesis inhibition, the importance of trehalose in insect physiology and development is investigated. Also, in two other lepidopterans, TPP inhibition impedes physiology and survival. NPP is also found to be effective as an insecticidal formulation. Overall, trehalose levels affect the larval size, weight, and metabolic homeostasis for larval‐pupal transition in lepidoptera.

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Regulation of trehalose metabolism in insects: from genes to the metabolite window

2023

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Trehalose is a major circulatory sugar in the haemolymph of insects. It provides instant energy and protection against stress. Trehalose metabolism is associated with insect growth and development. The architecture and spatio-temporal expression dynamics of trehalose metabolism and transport genes are key for regulation. These genes are controlled by various transcription factors, largely linked to nutrition, insect development, and metamorphosis. Also, trehalose levels are affected by substrate affinities and modifications of enzymes involved in the pathway. A feedback mechanism involving the precursors and products can regulate trehalose metabolism. Further, the neuroendocrine system controls trehalose levels under normal and stressed conditions by producing different hormones. Hypotrehalosemic hormones work under surplus energy conditions to activate haemolymph trehalose uptake and degradation. In contrast, hypertrehalosemic hormones stimulate trehalose production in the fat body and its transport to the haemolymph. However, trehalose metabolism regulation in insects needs to be studied in detail. This review discusses aspects of trehalose synthesis, transport, and degradation dynamics in developmental transition and stress response. Unravelling the epigenetic factors, transcriptional control and chemical or genetic modulators can provide further insights into the intricate regulation of trehalose in a development- and tissue-specific manner. This molecular information about effectors and regulators of trehalose metabolism can be applied in developing diverse biotechnological applications.

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Kinetic characterization and molecular modeling of trehalose-6-phosphate phosphatase from Anopheles gambiae and expressed in Pichia pastoris

Cited by 3 publications

References 58 publications

Trehalose‐6‐phosphate phosphatases are involved in trehalose synthesis and metamorphosis in Bactrocera minax

Trehalose‐6‐phosphate phosphatases are involved in trehalose synthesis and metamorphosis in Bactrocera minax

Inhibition of Trehalose Synthesis in Lepidoptera Reduces Larval Fitness

Regulation of trehalose metabolism in insects: from genes to the metabolite window

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