Kinetic characteristics of the energy production system in rat brain under conditions of posthypoxic encephalopathy and its correction withBergeniae crassifolia extract

Abstract

Posthypoxic encephalopathy is characterized by inhibition of the succinate oxidase stage in cerebral energy production. When administered to rats exposed to hypoxia, Bergeniae crassifolia extract reduced mortality and restricted the inhibition of rapid metabolic cluster reactions Key Words: Bergenia crassifolia extract; posthypoxic encephalopathy," brain mitochondria; NADHThis study analyzes the role of rapid and slow metabolic clusters [2,3] in the maintenance of brain energy homeostasis by measuring the level of NADH fluorescence in rat brain mitochondria during posthypoxic encephalopathy (PHE) and its correction with extract from Bergenia crassifolia leaves. Previous screening revealed high cerebroprotective activity of this preparation in hypoxia [4,5]. The present study used an original approach to the assessment of energy production based on the analysis of kinetic characteristics of pyridine nucleotide reduction (PNR) during phosphorylation of exogenous ADP [4]. MATERIALS AND METHODSThe study was carried out on 2-month-old male Wistar rats weighing 180-200 g (Laboratory of Experimental Biomedical Modeling, Tomsk Research Center).Piracetam (400 mg/kg) and extract of Bergenia crassifolia leaves (300 mg/kg) were suspended in water and administered intragastrically for 5 days starting from day 14 after hypoxia. Hypoxia was modeled in a hermetically sealed 5-liter jar until the start of agony (3.5-4.0 h), after which the jar was open, and the aniLaboratory of Molecular Pharmacology, Institute of Pharmacology, Tomsk Research Center. Siberian Division of the Russian Academy of Medical Sciences mal was returned to its home cage. After the 19th hypoxia session the rats were decapitated under ether anesthesia.Isolated brains were homogenized in an ice-cold medium containing: 1.2• ~ M KC1, 2x10 3 M K2CO3, l0 -2 M HEPES, 2x10 -4 M EDTA (pH=7.2), 2x10-3 M KH2PO 4 (pH=7.2 at 26~ was added during incubation. The functional state of the energy production system was evaluated by the level of reduced pyridine nucleotides [9].The data were analyzed statistically using paired Wilcoxon--Mann--Whitney test. RESULTSIn the experimental animals, the time of NAD reduction after the addition of ADP to cerebral mitochondria oxidizing an endogenous substrate was 2.65-fold prolonged and the rate of transition from 2AP to 2R metabolic state was 2.8 times lower compared to the control (Table 1 ). During oxidation of exogenous succinate, the time of NAD reduction was 2.3-fold prolonged and the rate of transition from metabolic state 3 to 4R was 2.5 times lower than the corresponding control values. During oxidation of NAD-dependent substrates, Tr 3 increased 1.95-fold and V 3 decreased 2.9-fold. These changes indicate that hypoxic injury causes pronounced negative shifts in the brain energy metabolism [ 1 ].

Adaptogenic effect of black and fermented leaves of Bergenia crassifolia L. in mice

“…This is interesting, in that utilization of lactate correlates with an increased content of bergenin in the B. crassifolia leaves. Bergenin is known to have an immunomodulatory effect (Nazir et al, 2007) and the prophylactic introduction of a Bergenia extract at 300 mg/kg increases the time of life of animals at hypoxia and prevents the development of pathological changes (Khazanov and Smirnova, 2000).…”

The result of "Gastrovit" preparation influence on bioelectrical activity of rat's stomach

“…Badaan's leaf activates phagocyte in case of "in vitro" and recovers immune in case of "in vivo" as bergenin belongs to galacturons [5]. Extract of Badaan's leaf protects the brain acid restriction and is approved [6]. In the earth top of Badaan contains complex caratinoide, rutine, Vitamin C, tanine, complex flavnoids, arbutines, celloide, antraglucoids, complex pectin and K 2O-23.6%, CaO, P2O5, MgO, SiO2, Fe 2O3, Al2O3, MnO, Na2O, TiO2, F [12].…”