Kinetic change of oxidative stress in cerebrospinal fluid of mice infected withAngiostrongylus cantonensis

Abstract

The cerebrospinal fluid (CSF) of C57BL/6 mice infected with Angiostrongylus cantonensis was examined for kinetic changes in oxidative stress parameters, including reactive oxygen species (ROS), superoxide dismutase (SOD), catalase, malondialdehyde (MDA), 8-isoprostane, and 8-hydroxy-2'-deoxyguanosine (8-OHdG). The ROS increased gradually in the early stage of infection. During days 12-30 post-infection, the infected mice revealed ROS levels significantly higher than that in uninfected controls (P < 0.001). The ROS levels peaked at day 24 and then returned to that observed in uninfected controls at day 45 post-infection. The kinetics of MDA, 8-isoprostane, and 8-OHdG concentration changes observed in the CSF of the infected mice corresponded with kinetic changes in ROS levels. Thus, the excess ROS caused lipid peroxidation and DNA damage to cells in the central nervous system (CNS) of mice infected with A. cantonensis despite the increased antioxidant SOD and catalase enzyme activities during post-infection days 12-30. The oxidative stress in the CNS of C57BL/6 mice was apparently increased by diseases associated with A. cantonensis infection.

Anti-apoptotic effects of Sonic hedgehog signalling through oxidative stress reduction in astrocytes co-cultured with excretory-secretory products of larval Angiostrongylus cantonensis

“…Moreover, parasitic protozoa may also cause DNA lesions and apoptosis via oxidative stress 20 21 22 23 24 25 26 27 . Moreover, ROS have also been detected in CSF of mice infected with A. cantonensis 28 . After co-culturing with ESPs, the levels of superoxide and hydrogen peroxide in astrocytes significantly increased ( Fig.…”

“…After infection with protozoan parasites such as Plasmodium falciparum, Leishmania infantum, Toxoplasma gondii and Trypanosoma cruzi , oxidative stress is induced in host cells, causing a wide range of effects, such as formation of DNA lesions and apoptosis 20 21 22 23 24 25 26 27 . However, oxidative stress and the expression of antioxidants such as glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-transferase (GST) have both been found to be elevated in the CSF of mice infected with A. cantonensis 28 29 . Although oxidative stress may aggravate meningitis and brain injuries, antioxidants may reduce the levels of damage due to oxidative stress.…”

Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis

“…In A. cantonensis infection, reactive stress and injury are elevated in the parenchyma, meninges, and cerebrospinal fluid of the mouse brain [ 37 , 38 ]. Moreover, this nematode induces blood-brain barrier dysfunction and immune responses in the host brain [ 39 ].…”

A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens

“…The upregulation of genes involved in DNA repair mechanisms is important in maintaining the integrity of DNA. DNA can be damaged under infection such as by reactive oxygen species (ROS) [26,27].…”