Kinetic and Temporal Factors Influence Chilling Injury to Germinal Vesicle and Mature Bovine Oocytes

Zeron, Yoel; Pearl, M.; Borochov, Amihud; Arav, Amir

doi:10.1006/cryo.1998.2139

Cited by 86 publications

(53 citation statements)

References 27 publications

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“…The viability or survival of oocytes was affected by both VS/WS and vitrification/warming procedures. FDA has been previously used to assess the viability of mouse embryos (Mohr & Trounson 1980), porcine GV oocytes (Didion et al 1990) and bovine oocytes (Zeron et al 1999). Oocyte viability was evidenced by intense fluorescence; nonfluorescence indicated nonviability.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, the oocytes were cultured in m-PBS 2 containing 2.5 mg fluorescein diacetate (FDA)/ml for 3 min at 38.5 8C and then washed three times in m-PBS 2 solution before examination of membrane integrity under an inverted fluorescence microscope by the method of Zeron et al (1999). High intensity of flourescence level was regarded as high viability, low fluorescence intensity was regarded as low viability, and nonfluorescence indicated dead oocytes.…”

Section: Assessment Of Viability and Survival Of Oocytesmentioning

confidence: 99%

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Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

Shi¹,

Zhu

Zhang³

et al. 2006

Reproduction

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This study was designed to examine the effect of Taxol pretreatment on vitrification of porcine oocytes matured in vitro by an open pulled straw (OPS) method. In the first experiment, the effect of Taxol pretreatment and fluorescein diacetate (FDA) staining on parthenogenetic development of oocytes was evaluated. In the second experiment, viability, microtubule organization and embryo development of oocytes were assessed after oocytes were exposed to vitrification/warming solutions or after vitrification with or without Taxol pretreatment. The results showed that Taxol pretreatment and/or FDA staining did not negatively influence the oocyte's developmental competence after parthenogenetic activation. After being exposed to vitrification/warming solutions, the survival rate (83.3%) of the oocytes was significantly (P < 0.05) reduced as compared with that in the control (100%). Vitrification/warming procedures further reduced the survival rates of oocytes regardless of oocytes being treated with (62.1%) or without (53.8%) Taxol. The proportions of oocytes with normal spindle configuration were significantly reduced after the oocytes were exposed to vitrification/warming solutions (38.5%) or after vitrification with (10.3%) or without (4.1%) Taxol pretreatment as compared with that in control (76.8%). The rates of two-cell-stage (5.6-53.2%) embryos at 48 h and blastocysts (0 -3.8%) at 144 h after activation were significantly reduced after exposure to vitrification/warming solutions or after vitrification as compared with control (90.9% and 26.6% respectively). However, the proportion of vitrified oocytes developed to two-cell stage was significantly higher when oocytes were pretreated with (24.3%) than without (5.6%) Taxol. These results indicate that pretreatment of oocytes with Taxol before vitrification helps to reduce the damage induced by vitrification and is a potential way to improve the development of vitrified porcine oocytes. Reproduction (2006) 131 795-804

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Section: Discussionmentioning

confidence: 99%

Section: Assessment Of Viability and Survival Of Oocytesmentioning

confidence: 99%

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

Shi¹,

Zhu

Zhang³

et al. 2006

Reproduction

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“…Guignot et al [6] reported that the period and temperature of ovary storage affected the quality of oocytes, in which cytoplasmic membrane [30,31], microtubule [32], cytoskeleton [33] and zona pellucida [34] may be sensitive to low temperatures. It has been known that porcine oocytes are highly sensitive to chilling, resulting in a loss of membrane integrity when cooled below 16 C [35]. In bovine oocytes, the membrane lipid of oocytes at the GV stage have a transition phase at temperatures between 13 C and 20 C and the plasma membrane of oocytes is very sensitive to cooling at or below 20 C [36].…”

Section: Discussionmentioning

confidence: 99%

Effects of Ovary Storage Time and Temperature on DNA Fragmentation and Development of Porcine Oocytes

Wongsrikeao

Otoi

Karja

et al. 2005

Journal of Reproduction and Development

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Abstract. The present study was conducted to investigate the effects of storage time and temperature of porcine ovaries on the quality and nuclear maturation in vitro of oocytes obtained from stored ovaries and their subsequent development after in vitro fertilization. The ovaries were stored in physiological saline for 0, 3, 6, 9 and 12 h at various temperatures (4, 15, 25 and 35 C). The pH of follicular fluid obtained from the ovaries, DNA fragmentation of the oocyte nucleus and meiotic competence of oocytes were examined. Some oocytes from ovaries stored at 15, 25 and 35 C for 6 h were fertilized in vitro, and then cultured for 7 days to examine the ability of embryos to develop to the blastocyst stage. When the ovaries were stored at 35 C, the pH of follicular fluid decreased and the proportions of oocytes with DNA fragmented nuclei increased as the storage time was prolonged, and the storage of ovaries for 6, 9 and 12 h resulted in lower maturation rates of oocytes. When the ovaries were stored at 4, 15, 25 and 35 C for 6 h, the storage at higher temperatures (≥15 C) decreased the pH of follicular fluid and induced nucleic DNA fragmentation in higher proportions of oocytes. None of the oocytes from ovaries stored at 4 C reached metaphase II. The storage of ovaries at 15 C reduced the rates of in vitro fertilized oocytes and subsequent embryo development, but there were no significant differences in the rates of fertilization and blastocyst formation between oocytes from ovaries stored at 25 C and 35 C. Our findings indicate that the storage of ovaries at 25-35 C for 6 h is effective for maintaining the developmental competence of porcine oocytes even though the development rates were lower than those of ovaries stored at 35 C for 3 h.

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“…We obtained blastocysts only from oocytes vitrified after IVM. The chilling sensitivity of the mammalian oocyte is well documented [1,26,27], and consequently success to survive cryopreservation is limited. Cryopreservation involves controlling many variables, such as cryoprotectant (CP) type used, the method for adding and removing CPs, and cooling and thawing rates, each of these factors being a possible source of cell damage.…”

Section: Discussionmentioning

confidence: 99%

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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The nuclear stage at which oocytes are cryopreserved influences further development ability and cryopreservation affects ultrastructure of both cumulus cells and the oocyte. In this work, we analyze the effects of vitrification at different nuclear and cytoplasmic maturation stages on the oocyte ultrastructure and developmental ability. Culture in TCM199 + PVA with roscovitine 25 M during 24 h led to meiotic arrest (MA) in cumulus-oocyte complexes (COCs), while permissive in vitro maturation (IVM) was performed in TCM199, 10% FCS, FSH-LH and 17b-estradiol for 24 h. Oocytes were vitrified using the open pulled straw method (OPS) with minor modifications. Fresh and vitrified/warmed COCs were fixed as immature, after IVM, after meiotic arrest (MA) and after MA + IVM.Vitrification combined with MA followed by IVM produced the highest rates of degeneration, regardless of the vitrification time. As a consequence, lower proportions of embryos cleaved in these groups, although differences were eliminated at the five-eight cell stage. Development rates up to day 8 were similar in all experimental groups, being significantly lower than those in fresh controls. Only oocytes vitrified after IVM were able to give blastociysts. The morphological alterations observed can be responsible for compromised development. More research is needed to explain the low survival rates of the bovine oocyte after vitrification and warming. #

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Kinetic and Temporal Factors Influence Chilling Injury to Germinal Vesicle and Mature Bovine Oocytes

Cited by 86 publications

References 27 publications

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

Effects of Ovary Storage Time and Temperature on DNA Fragmentation and Development of Porcine Oocytes

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

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