“…S9 ). Mutations in Glu142 in M. tuberculosis ADPRase and Glu166 in human NUDT5 considerably decrease k cat , indicating that they are involved in the deprotonation of the water molecule ( 30 , 45 ). However, mutational analysis of T. thermophilus ADPRase revealed that the mutants, Asp126, Glu127, Asp128, and Glu129, on the equivalent loop retain normal activity ( 46 ).…”