Kinetic and Magnetic Resonance Studies of Pyruvate Kinase. III. The Enzyme-Metal-Phosphoryl Bridge Complex in the Fluorokinase Reaction<sup>*</sup>

Mildvan, Albert S.; Leigh, John S.; Cohn, Mildred

doi:10.1021/bi00858a032

Cited by 112 publications

(23 citation statements)

References 29 publications

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“…a Calculated according to scheme (4) and equation (5). In an analogous way the values for log hlE,Y$' $i(L, can also be calculated.…”

Section: 65mentioning

confidence: 99%

“…The importance of mixed complexes in nature is evident, since a great deal of biochemical reactions occur in such complexes, i.e. within the coordination sphere of metal ions [3,4].I n addition, the investigation of ternary Cu2+ complexes, containing an imidazole group as binding site, is of special interest, since it is suggested for several proteins which interact with Cua+ and for natural Cu-proteins that the imidazole group of histidine binds to copper. Thus, the Cuz+ complexes of several histidine-containing peptides …”

mentioning

confidence: 99%

“…mixed ligand metal ion complexes, can be considered as models [2] for enzyme-metal ion-substrate complexes [3,4]. Furthermore, such ternary complexes probably occur in biological fluids that contain several ligands as well as different metal ions.…”

mentioning

confidence: 99%

“…The importance of mixed complexes in nature is evident, since a great deal of biochemical reactions occur in such complexes, i.e. within the coordination sphere of metal ions [3,4].…”

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confidence: 99%

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Ternary Complexes in Solution

Huber

Griesser

Prijs

et al. 1969

European Journal of Biochemistry

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The stability constants of the ternary Cuz+ complexes, ethylenediamine-Cu2+-pyrocatechol and histamine-Cua+-pyrocatechol dianion, were determined. Both complexes are more stable than one would expect on pure statistical reasons. The imidazole-containing ternary Cuz+ complex is, however, especially stable. For the equilibrium, Cu(histamine), + Cu(pyrocatechol), ~2[(histamine)Cu(pyrocatechol)], the constant is log X = 4.86 (I = 0.1; T = 25"), while the statistical value is 0.6. These results were compared with those taken (or calculated) from the literature; it is concluded that the n-system of the ligands is important for such a great increase in stability. Furthermore, the Cuz+-histamine I : 1 complex selectively binds to 0-ligands rather than to N-ligands. Thus, the first coordinated ligand has an influence on the kind of the second to be coordinated, i. e. the Cua+-histamine 1 : 1 complex shows discriminating qualities. The significance of these results is discussed with regard to Cu-proteins, since it is suggested for several enzymes that the imidazole group of histidine is involved with the binding of metal ions.I n general, ternary complexes, i.e. mixed ligand metal ion complexes, can be considered as models [2] for enzyme-metal ion-substrate complexes [3,4]. Furthermore, such ternary complexes probably occur in biological fluids that contain several ligands as well as different metal ions. Investigations of the stability of ternary complexes may, therefore, help toward understanding the driving forces which lead to the formation of such complexes in biological systems. The importance of mixed complexes in nature is evident, since a great deal of biochemical reactions occur in such complexes, i.e. within the coordination sphere of metal ions [3,4].I n addition, the investigation of ternary Cu2+ complexes, containing an imidazole group as binding site, is of special interest, since it is suggested for several proteins which interact with Cua+ and for natural Cu-proteins that the imidazole group of histidine binds to copper. Thus, the Cuz+ complexes of several histidine-containing peptides

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“…a Calculated according to scheme (4) and equation (5). In an analogous way the values for log hlE,Y$' $i(L, can also be calculated.…”

Section: 65mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

“…The importance of mixed complexes in nature is evident, since a great deal of biochemical reactions occur in such complexes, i.e. within the coordination sphere of metal ions [3,4].…”

mentioning

confidence: 99%

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Ternary Complexes in Solution

Huber

Griesser

Prijs

et al. 1969

European Journal of Biochemistry

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“…This involves the formation of chelate complexes of toxic metal ions with administered drugs which can be easily excreted via urine or feces (Chisolm,1968;Goyer & Cherian, 1978;May and Williams, 1979;Bulman et al, 1979;Das, 1990;Wang et.al.,2006). On the other hand, single and mixed ligand chelates of metals with various ligand molecules have been studied extensively related to various aspects quantitatively (Nakayama et.al., 1989;Florence 1982;Mildvan et al,1967;Shelke & Jahagirdar,1976;Maktell and Carey,1967;Moustafa, 2005) and many of the mixed chelate systems of bio-molecules with biologically important metals like Cu, Ni and Zn investigated were used as models of biochemistry to understand more complicated biological reactions (Mildvan & Cohn, 1966;El-Haty et al,1995;Brumas et al,1995;Kleszczewska, 1999;Bucci, 2000).…”

Section: Introductionmentioning

confidence: 99%

Study on mixed ligand complexes of Pb(II) with EDTA and dietary ligands to investigate the applicability on chelation therapy

Chandrathilaka

Hettiarachchi

2017

Ceylon J. Sci.

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Lead is the most distributed toxic metal in the world and chelation therapy is a remedy for removing it from the body, if a person is intoxicated. Stability constants of both binary and mixed ligand complexes of Pb(II) with salicylic acid, ascorbic acid, vitamin B 1 (thiamine), vitamin B 3 (niacin) and Na 2 EDTA were potentiometrically studied, in aqueous media at the temperature of 37.0 ± 0.2 ⁰ C and the ionic strength of 0.15 mol dm -3 in order to identify better mixed ligand systems suitable for chelation therapy of lead. Both metal-ligand and proton-ligand formation constants were calculated using IrvingRossotti equations. The Formation of ternary complexes was further confirmed theoretically by plotting composite curves. Δ log K values calculated revealed that all mixed complexes were more stable than the complexes of corresponding binary systems. EDTA-Vitamin B 1 and EDTA-Ascorbic mixed complexes with Pb(II) disclosed very high stability constants, which were two times greater than that of EDTA binary complex. Vitamin B 1 revealed a remarkably high ability in forming mixed ligand complexes with all other four ligands. Results of this work clearly indicate that (EDTA-Vitamin B 1 ), (EDTA-Ascorbic), (Vitamin B 1 -Ascorbic), (Vitamin B 1 -Salicylic) and (Vitamin B 1 -Vitamin B 3 ) combinations can be used for detoxification of lead more effectively using dietary ligands.

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Mildvan, Albert S.: Early Applications to Enzymes of Paramagnetic Effects on Nuclear Relaxation

Mildvan¹

2007

Encyclopedia of Magnetic Resonance

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Kinetic and Magnetic Resonance Studies of Pyruvate Kinase. III. The Enzyme-Metal-Phosphoryl Bridge Complex in the Fluorokinase Reaction^*

Cited by 112 publications

References 29 publications

Ternary Complexes in Solution

Ternary Complexes in Solution

Study on mixed ligand complexes of Pb(II) with EDTA and dietary ligands to investigate the applicability on chelation therapy

Mildvan, Albert S.: Early Applications to Enzymes of Paramagnetic Effects on Nuclear Relaxation

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Kinetic and Magnetic Resonance Studies of Pyruvate Kinase. III. The Enzyme-Metal-Phosphoryl Bridge Complex in the Fluorokinase Reaction*

Cited by 112 publications

References 29 publications

Ternary Complexes in Solution

Ternary Complexes in Solution

Study on mixed ligand complexes of Pb(II) with EDTA and dietary ligands to investigate the applicability on chelation therapy

Mildvan, Albert S.: Early Applications to Enzymes of Paramagnetic Effects on Nuclear Relaxation

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Kinetic and Magnetic Resonance Studies of Pyruvate Kinase. III. The Enzyme-Metal-Phosphoryl Bridge Complex in the Fluorokinase Reaction^*