Kinetic and immunohistochemical characteristics of mitogen-induced cutaneous hypersensitivity in chickens selected for antibody responsiveness

Parmentier, H.K.; Reilingh, G. de Vries; Nieuwland, M.G.B.

doi:10.1016/s0165-2427(98)00200-1

Cited by 42 publications

(55 citation statements)

References 16 publications

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“…PHA first induces an acute response 4·h after injection, primarily characterised by oedema. Then it induces a delayed-type hypersensitivity response through stimulating heterophils, basophils, granulocytic and mononuclear cell infiltration in dermis and dense perivascular infiltration of T lymphocytes at the site of injection (Parmentier et al, 1998;Sharma, 1990). This late response generally peaks 18·h after injection, and may last up to 36·h.…”

Section: Data Collection and Experimental Protocolmentioning

confidence: 99%

Carotenoid availability in diet and phenotype of blue and great tit nestlings

Biard

Surai²,

Møller

2006

Journal of Experimental Biology

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Carotenoids are biologically active pigments of crucial importance for the development of avian embryos and nestlings. Thus parental ability to provide nestlings with a carotenoid-rich diet may enhance offspring fitness. However, very little is known about the possible effects of carotenoid availability in the diet on growing nestlings in natural populations. We experimentally manipulated dietary intake of carotenoids by nestlings of two closely related passerine species, the great tit Parus major and the blue tit Parus caeruleus, and measured nestling antioxidants, body condition, immunity and plumage colour. There was no detectable increase in plasma carotenoids after treatment in carotenoid-fed nestlings of either species despite regular supply of dietary carotenoids. However, in carotenoid-fed blue tit nestlings, plasma vitamin E concentration increased with plasma carotenoid concentration, while that was not the case for control nestlings. In both species, there was no significant effect of carotenoid supply on immune function. Carotenoid supplementation enhanced yellow feather colour in great tit nestlings only. In both species a strong effect of carotenoid supply was found on body condition with an increase in body mass for small carotenoid-fed nestlings compared to similarly sized control nestlings. Dietary availability of carotenoids may thus have important fitness consequences for tits. We hypothesise that the difference in effect of dietary carotenoids on the two species is due to relatively larger clutch size and higher growth rates of blue tits compared to great tits, leading to blue tit nestlings being more in need of carotenoids for antioxidant function than great tit nestlings

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Section: Data Collection and Experimental Protocolmentioning

confidence: 99%

Carotenoid availability in diet and phenotype of blue and great tit nestlings

Biard

Surai²,

Møller

2006

Journal of Experimental Biology

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“…PHA first induces an acute response 4 h after injection, primarily characterised by oedema. Then a delayed-type hypersensitivity response is induced through stimulating heterophil, basophil, eosinophil, macrophage and thrombocyte cell infiltration in the dermis and dense perivascular infiltration of T-lymphocytes at the site of injection (Sharma, 1990;Parmentier et al, 1998;Martin et al, 2006). This late response generally peaks 18 h after injection and may last up to 36 h. This test therefore reflects the combined responses of T-cells, cytokines and inflammatory cells (Davison et al, 1996) and involves both innate and adaptive components of the immune system (Martin et al, 2006;Tella et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of PHA-induced immune response and plasma carotenoids in birds:should we have a closer look?

Biard

Hardy

Motreuil

et al. 2009

Journal of Experimental Biology

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SUMMARYAllocation trade-offs of limited resources are thought to ensure the honesty of sexual signals and are often studied using controlled immune challenges. One such trade-off between immunity and ornaments is that involving carotenoids. Phytohemagglutinin (PHA)-induced immune response is a widely used immune challenge, yet more details on the underlying physiological mechanisms and potential costs are needed. We investigated the temporal dynamics of PHA-induced immune response and associated changes in blood carotenoids, body mass and a carotenoid-based coloured signal. We found variation in individual response patterns to PHA after peak swelling was reached, with birds showing either a rapid or a slow subsequent decrease in swelling, suggesting variation in the duration of the immune response and/or inflammation. Body mass did not affect immune response. Plasma carotenoids followed a transient decrease closely matching the dynamics of the swelling. The peak of the immune response was negatively related to initial plasma carotenoid levels and positively correlated to the relative decrease in plasma carotenoids. Individual variation in duration of the swelling could be partly explained by plasma carotenoids; high initial carotenoid levels were associated with a slower decrease of the swelling. These contradictory effects of carotenoids suggest a complex role in the immune response. Bill colour was positively correlated to initial plasma carotenoid concentration but it did not predict or change as a consequence of immune response to PHA. Bill colour thus reflects medium-or long-term quality rather than immediate quality. Taking into account the dynamics of the immune response and that of associated physiological parameters would thus yield new insights into our interpretation of variation in PHA response.

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“…in the wing web), and with the same amount of saline buffer, we tried to minimize variation between immune treatment groups in local inflammation as a direct result of tissue damage. We measured the thickness change at the injection site (a measure of the inflammatory response) 4.0 ± 0.1 h after an injection of LPS and 24.5 ± 0.3 h after an injection of PHA (Parmentier et al 1998;Smits et al 1999). The 4-h delay was chosen for LPS because it corresponds to the peak of the cutaneous response following an injection of LPS in domestic fowls (Gallus gallus domesticus; Parmentier et al 1998).…”

Section: Experimental Designmentioning

confidence: 99%

“…We measured the thickness change at the injection site (a measure of the inflammatory response) 4.0 ± 0.1 h after an injection of LPS and 24.5 ± 0.3 h after an injection of PHA (Parmentier et al 1998;Smits et al 1999). The 4-h delay was chosen for LPS because it corresponds to the peak of the cutaneous response following an injection of LPS in domestic fowls (Gallus gallus domesticus; Parmentier et al 1998). Likewise, the 24 h delay corresponds to a peak of the cutaneous response to PHA in chicken (Parmentier et al 1998) and house sparrows (Passer domesticus; Martin II et al 2003).…”

Section: Experimental Designmentioning

confidence: 99%

“…In tawny owls, fledging body mass positively correlates with survival before dispersal (Overskaug et al 1999), implying a selective advantage to nestlings that maintain body mass before leaving the nest. We choose PHA and LPS skin-swelling tests because of their amenability to fieldwork and because the immune responses induced by these two immunostimulants are well studied (Dantzer et al 1998;Parmentier et al 1998;Merino et al 1999;Martin II et al 2006;Tella et al 2009). PHA is derived from plant beans of Phaseolus vulgaris and specifically induces T-cell division in vitro (Mire-Sluis et al 1987).…”

Section: Introductionmentioning

confidence: 99%

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Indirect Costs of Parasitism are Shaped by Variation in the Type of Immune Challenge and Food Availability

et al. 2010

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Parasites can inflict indirect fitness costs to their hosts by eliciting costly immune responses. These costs depend on the type and amount of immunostimulants presented to the host immune system but also on the amount of resources available to fuel host immune responses. Here, we investigated how the relative costs of two different types of immune challenge are modulated by variation in food availability. We injected nestling tawny owls (Strix aluco) with either 10 lg of phytohaemagglutinin (PHA) or 20 lg of lipopolysaccharide (LPS), and subsequently raised them under two different food regimes (food-restricted vs. ad libitum). After controlling for food consumption, we found that LPS-injected nestlings lost more body mass than PHA-injected ones only when foodrestricted. We also found that body mass gain of owlets fed ad libitum decreased with the intensity of the skin swelling response against LPS, but not PHA. These experimental and correlative results suggest that nestling tawny owls suffered greater immune costs when treated with LPS than PHA, and that variation in the costs of two different types of immune challenge can be exacerbated under conditions of low food availability. Our study highlights the importance of taking into consideration the interplay between host immunity and nutrition in the study of indirect costs of parasitism.

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Kinetic and immunohistochemical characteristics of mitogen-induced cutaneous hypersensitivity in chickens selected for antibody responsiveness

Cited by 42 publications

References 16 publications

Carotenoid availability in diet and phenotype of blue and great tit nestlings

Carotenoid availability in diet and phenotype of blue and great tit nestlings

Dynamics of PHA-induced immune response and plasma carotenoids in birds:should we have a closer look?

Indirect Costs of Parasitism are Shaped by Variation in the Type of Immune Challenge and Food Availability

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