Kinetic analysis of the free-radical-induced lipid peroxidation in human erythrocyte membranes: Evaluation of potential antioxidants using cis-parinaric acid to monitor peroxidation

McKenna, R; Kézdy, Ferenc J.; Epps, Dennis E. Van

doi:10.1016/0003-2697(91)90492-c

Cited by 46 publications

(15 citation statements)

References 11 publications

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“…This assay colorimetrically measures malondialdehyde (MDA), an end product formed from a variety lipid peroxides and secondary lipid oxidation products. However, the TBA assay does not give quantitative information since a large number of biological molecules interfere with the generation of MDA [16]. When PnA was used as a monitor molecules for the assessment of lipid peroxidation in the cells, the results showed that lipid peroxidation was induced by ROSs after isolation of the cells from the bursal follicles.…”

Section: Discussionmentioning

confidence: 95%

“…A cis-parinaric acid (PnA) was used as a monitor molecule for the assessment of lipid peroxidation [16]. PnA is fluorescent when partitioned into a lipid environment and the emission of fluorescence disappears upon reaction of the lipid with ROSs.…”

Section: Resultsmentioning

confidence: 99%

“…A cis-parinaric acid (PnA) was used as a monitor molecule for the assessment of lipid peroxidation [16]. Briefly, PnA was dissolved in DMSO at a concentration of 10 mM and saturated with N2 gas.…”

Section: Assessment Of Lipid Peroxidationmentioning

confidence: 99%

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Effects of Antioxidants on Induction of Apoptosis in Bursal Cells of Fabricius during In Vitro Cultivation

Arai

Itoh

Kanda

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. After physically disrupting cell contacts, apoptosis of bursal cells of Fabricius was induced during in vitro cultivation. The percentage of apoptotic cells increased with incubation time and approximately 70% cells represented apoptosis after 6 hr of incubation. The induction of apoptosis was significantly inhibited by treatment of the cells with ascorbic acid (vitamin C), but not with trolox, a vitamin E analog. An intense DNA ladder pattern was shown at 6 hr post-isolation, which is a biochemical hallmark of apoptosis. Treatment of the cells with ascorbic acid inhibited the DNA fragmentation, but trolox did not. To monitor the intracellular production of reactive oxygen species (ROSs), the intensity of fluorescence emitted from DCFH-DA was measured. The intensity of fluorescence from cells incubated for 0.5-2 hr was approximately 2-fold higher than that from cells at 0 hr. The relative intensity of fluorescence decreased immediately after the addition of ascorbic acid to the cells. The intensity from the cells treated with ascorbic acid was 20-30% of that from the control cells at each incubation time. For trolox, the intensity was 50-70% of that from the control cells at each 1 to 2 hr incubation time. When ROSs-induced lipid peroxidation was assessed using cis-parinaric acid (PnA) as a monitor molecule, lipid peroxidation was found to occur in the control cells after isolation of the bursal cells. Treatment of the cells with trolox reduced lipid peroxidation, but treatment with ascorbic acid enhanced peroxidation.-KEY WORDS: antioxidant, apoptosis, bursa of Fabricius, in vitro culture, reactive oxygen species.

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Section: Discussionmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

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Effects of Antioxidants on Induction of Apoptosis in Bursal Cells of Fabricius during In Vitro Cultivation

Arai

Itoh

Kanda

et al. 2000

The Journal of Veterinary Medical Science

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“…Регистрация флуоресценции цис-паринариевой кислоты в присутствии липидов, экстрагированных из эритроцитов крови крыс. Для регистрации флуоресценции цис-паринариевой кислоты в присутствии липидов, экстрагированных из эритроцитов крови крыс, использовали стандартную методику [12][13][14][15]20] …”

Section: методикаunclassified

“…Для этого мы использовали метод, основанный на измерении флуоресценции цис-паринариевой кислоты (цисПК) [12][13][14][15]20]. Известно, что флуоресценция цисПК снижается при её окислении свободными радикалами.…”

Section: при обработке результатов рассчитывали процент падения флуорunclassified

Effects of low power laser radiation of blue, green and red ranges on free radical processes in rat blood in endotoxic shock

IuA

et al. 2013

BIOMED KHIM

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Целью данной работы было исследование влияния низкоинтенсивного лазерного излучения синего (441,2 нм), зелёного (532,5 нм) и красного (632,8 нм) диапазонов на свободнорадикальные процессы в крови крыс при экспериментальном эндотоксическом шоке. В работе использовали модель эндотоксического шока, получаемую интраперитонеальным введением липополисахарида В (25 мг/кг). Были проведены измерения функциональной активности лейкоцитов крови (методом люминол-зависимой хемилюминесценции), супероксиддисмутазной активности плазмы крови (методом с нитросиним тетразолием) и окисления липидов мембран эритроцитов (по измерению флуоресценции цис-паринариевой кислоты). Обнаружено, что низкоинтенсивное лазерное излучение оказывает сильное влияние на все исследуемые процессы как в группах животных, которым вводили липополисахарид В, так и в контрольных группах. Наиболее выраженное действие излучения наблюдали в группах животных, которым вводили липополисахарид. В этих группах лазерное излучение вызывало возрастание всех исследуемых показателей. При дозе облучения (0,75 Дж/см 2 ) наиболее эффективным оказалось излучение в зеленом диапазоне спектра. При дозе 1,5 Дж/см 2 сильное влияние оказывало излучение, как в зелёной, так и в красной областях спектра. Обсуждаются возможные механизмы наблюдаемых процессов.Ключевые слова: эндотоксический шок, низкоинтенсивное лазерное излучение, хемилюминесценция, активные формы кислорода, лейкоциты, супероксиддисмутазная активность, свободнорадикальное окисление липидов.ВВЕДЕНИЕ. Сепсис (эндотоксический шок, синдром системного воспаления (ССВ)) и последующий синдром полиорганной недостаточности (СПН) являются наиболее серьезными проявлениями внедрения бактериальной инфекции. Сегодня СПН -одна из частых причин смерти и довольно распространенный патофизиологический процесс. Около 1% всех больных и до 20-30% больных в реанимационных палатах так или иначе имеют 411 * -адресат для переписки

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Analytical and numerical techniques for the evaluation of free radical damage in cultured cells using scanning laser microscopy

Sawada¹,

Raub²,

Decker³

et al. 1996

Cytometry

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Scanning laser microscopy (SLM) was used to develop an assay to visualize the generation of intra‐cellular reactive oxygen species (ROS) and to evaluate the effect of the lipophilic antioxidant U‐87,663 on ROS formation. Cultured N18 neuroglioma cells were challenged by extracellular addition of cumene hydroperoxide, and subsequent intracellular generation of ROS was characterized by measuring the fluorescence intensity of the ROS indicator 2′,7′‐dichlorofluorescein (DCF). The kinetics of the reaction between ROS and the indicator DCF, or the antioxidant U‐87,663, can be most accurately assessed if results from individual cell clusters are analyzed independently. It is possible and necessary to account for these experimental and analytical properties in order to characterize the properties of the antioxidant activity precisely. We determined that the temporal increase in DCF fluorescence was consistent with the reaction of DCF with free radicals generated from cumene hydroperoxide, as was the loss of fluorescence from U‐87,663. The rate constants for the free radical reactions revealed that ROS reaction with DCF is 10 times faster than with U‐87,663. These differences in reaction rates combined with differences in the cellular distribution of the ROS indicator DCF, the antioxidant U‐87,663, and the bulk of the ROS prevented detection of any protection U‐87,663 may offer. © 1996 Wiley‐Liss, Inc.

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Kinetic analysis of the free-radical-induced lipid peroxidation in human erythrocyte membranes: Evaluation of potential antioxidants using cis-parinaric acid to monitor peroxidation

Cited by 46 publications

References 11 publications

Effects of Antioxidants on Induction of Apoptosis in Bursal Cells of Fabricius during In Vitro Cultivation

Effects of Antioxidants on Induction of Apoptosis in Bursal Cells of Fabricius during In Vitro Cultivation

Effects of low power laser radiation of blue, green and red ranges on free radical processes in rat blood in endotoxic shock

Analytical and numerical techniques for the evaluation of free radical damage in cultured cells using scanning laser microscopy

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