Kinetic analysis of bile salt passage across a dialysis membrane in the presence of cereal soluble dietary fibre polymers

Gunness, Purnima; Flanagan, Bernadine M.; Shelat, Kinnari J.; Gilbert, Robert G.; Gidley, Michael J.

doi:10.1016/j.foodchem.2012.03.131

Cited by 29 publications

(22 citation statements)

References 28 publications

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“…This describes the simplest model for diffusion under dialysis [16,19]. According to Equation (1), the concentration of taurocholic acid after reaching equilibrium (C f ) and the apparent permeability rate constant (k) were calculated by non-linear regression (Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…The impact of adsorptive and viscous effects can be displayed by correlating the apparent permeability rate (k) and the viscosity (Figure 4). Since only small shearing forces occur in the gastrointestinal tract, the correlation was based on the shear viscosity at a shear rate of 15 s −1 [16]. …”

Section: Resultsmentioning

confidence: 99%

“…Other approaches include pressure filtration and dialysis to separate unbound bile acids. Some in vitro studies have already demonstrated the suitability of dialysis to determine bile acid release from simulated chymes [15,16,17,18]. Little account has been taken of whether this approach can reflect adsorption as well as viscous properties of dietary fibres.…”

Section: Introductionmentioning

confidence: 99%

“…Correspondingly, first-order kinetics can be applied to the analysis of bile acid release across a dialysis membrane. This could enable the evaluation of viscous and adsorptive effects of dietary fibres based on the parameters of diffusion kinetics [16,19]. In the present study, we evaluated this approach by dialysis of in vitro digested chyme containing dietary fibre from different sources (i.e., barley, citrus, lupin, and potato) and studied adsorptive as well as viscous effects under simulating conditions of the small intestine.…”

Section: Introductionmentioning

confidence: 99%

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Differentiation of Adsorptive and Viscous Effects of Dietary Fibres on Bile Acid Release by Means of In Vitro Digestion and Dialysis

Naumann

Schweiggert‐Weisz

Bader-Mittermaier

et al. 2018

IJMS

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To explain the cholesterol-reducing effects of dietary fibres, one of the major mechanisms proposed is the reduced reabsorption of bile acids in the ileum. The interaction of dietary fibres with bile acids is associated with their viscous or adsorptive effects. Since these fibre characteristics are difficult to investigate in vivo, suitable in vitro methodologies can contribute to understanding the mechanistic principles. We compared the commonly used centrifugal approach with a modified dialysis method using dietary fibre-rich materials from different sources (i.e., barley, citrus, lupin, and potato). Digestion was simulated in vitro with oral, gastric, and small intestinal digestion environments. The chyme was dialysed and released bile acids were analysed by high-performance liquid chromatography. The centrifugation method showed adsorptive effects only for cholestyramine (reference material) and a high-fibre barley product (1.4 µmol taurocholic acid/100 mg dry matter). Alternatively, the dialysis approach showed higher values of bile acid adsorption (2.3 µmol taurocholic acid/100 mg dry matter) for the high-fibre barley product. This indicated an underestimated adsorption when using the centrifugation method. The results also confirmed that the dialysis method can be used to understand the influence of viscosity on bile acid release. This may be due to entrapment of bile acids in the viscous chyme matrix. Further studies on fibre structure and mechanisms responsible for viscous effects are required to understand the formation of entangled networks responsible for the entrapment of the bile acids.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differentiation of Adsorptive and Viscous Effects of Dietary Fibres on Bile Acid Release by Means of In Vitro Digestion and Dialysis

Naumann

Schweiggert‐Weisz

Bader-Mittermaier

et al. 2018

IJMS

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“…Nishimura et al has reported that Chinese yam contains 1.9% (w/w) of the SDF (31). Proposed biological mechanisms for the Chol-lowering effects of SDF include (1) increased barrier properties of the unstirred layer between micelles and intestinal absorptive cells, (2) molecular associations of SDF and bile salt, and (3) formation of a local SDF matrix that entraps bile salt micelles (32)(33)(34)(35). In addition, we also confirmed using a Megazyme Resistant Starch Assay Kit (Megazyme International Ltd.) that the present freeze-dried Jin contained 45.4% (w/w) resistant starch.…”

Section: Jin Blocks Lipid Absorption In the Intestines And Decreases mentioning

confidence: 99%

Consumption of Japanese Yam Improves Lipid Metabolism in High-Cholesterol Diet-Fed Rats

Kusano

Tsujihara

Masui

et al. 2016

J Nutr Sci Vitaminol

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SummaryWe investigated the effects of dietary Japanese yam (Dioscorea japonica Thunb.) on lipid metabolism. Male Wistar rats (6 wk old) were fed a high-cholesterol diet for 6 wk and then supplemented with 26% of Japanese yam or 0.5% of its constituent diosgenin for a further 4 wk of high-cholesterol feeding (C6-J4 and C6-D4 groups, respectively). In the C6-J4 group, body weight gains significantly decreased, but skeletal muscle fiber sizes in quadriceps significantly increased compared with the other groups. Furthermore, Japanese yam supplementation resulted in the reduction of triglyceride contents in their liver, quadriceps, and intra-abdominal visceral fat. Diosgenin supplementation resulted in an increase in the numbers of skeletal muscle fibers and decrease in the fat accumulations in liver and of the lipid contents in quadriceps. Although quadriceps cholesterol contents decreased concomitantly with increased serum HDL-cholesterol in both the groups, fecal bile acid, fecal cholesterol contents, and fecal weight were higher in the C6-J4 group than in the C6-D4 group. Meanwhile, we demonstrated that Japanese yam inhibited micellar cholesterol solubility in vitro in a concentration-dependent manner. These results suggest that Japanese yam is more effective than diosgenin in reducing fat accumulation and improving cholesterol metabolism during chronic consumption of a high-cholesterol diet.

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Solubility, Permeability, and Their Interplay

Beig

Marković

Dahan

2018

Early Drug Development

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Kinetic analysis of bile salt passage across a dialysis membrane in the presence of cereal soluble dietary fibre polymers

Cited by 29 publications

References 28 publications

Differentiation of Adsorptive and Viscous Effects of Dietary Fibres on Bile Acid Release by Means of In Vitro Digestion and Dialysis

Differentiation of Adsorptive and Viscous Effects of Dietary Fibres on Bile Acid Release by Means of In Vitro Digestion and Dialysis

Consumption of Japanese Yam Improves Lipid Metabolism in High-Cholesterol Diet-Fed Rats

Solubility, Permeability, and Their Interplay

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