Abstract:steps. Structural dynamics of dynein's AAA þ ring domain during processive stepping are not well understood. We use a combination of polarized TIRF and sub-pixel particle tracking to measure the position and orientation of fluorescent nanorods rigidly attached to AAA5 and AAA6 of the individual dynein ring domains via biotin-NeutrAvidin linkage. We observe rotational changes of the ring and how they correlate with translocation steps. The dynein ring undergoes frequent, small rotations, typically less than 20 … Show more
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