Kinderkardiologie

Haas, Nikolaus; Kleideiter, Ulrich

doi:10.1055/b-006-160284

Cited by 5 publications

(4 citation statements)

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“…In addition, photostability, solubility in aqueous solutions, low cytotoxicity, and small molecular size (relevant for super-resolution imaging) are also high desirable characteristics [1][2]. These probes should be excitable with the available light sources and showing high brightness to give rise to contrasted images.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO)₃⁺complexes with an electron withdrawing ancillary ligand

et al. 2016

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Research in fluorescence microscopy presents new challenges, especially with respect to the development of new metal-based fluorophores. In this work, the news fac-[Re(CO) 3 (bpy)L]PF 6 (C3) and fac-[Re(CO) 3 (dmb)L]PF 6 (C4) complexes, where L is an ancillary ligand E-2-((3-amino-pyridin-4-ylimino)-methyl)-4,6-diterbutylphenol, both exhibiting an intramolecular hydrogen bond, have been synthesized for its use as preliminary probes for fluorescence microscopy. The complexes were characterized using chemical techniques such as UV-Vis, 1 H-NMR, TOCSY, FT-IR, cyclic voltammetry, mass spectra (EI-MS 752.22 M + for C3 and 780.26 M + for C4) and DFT calculations including spin-orbit effects. The electron withdrawing nature of the ancillary ligand L in C3 and C4 explains their electrochemical behavior, which shows the oxidation of Re I at 1.84 V for C3 and at 1.88 V for C4. The UV-vis absorption and emission properties have been studied at room temperature in acetonitrile solution. The complexes show luminescent emission with a large Stokes shift (λ ex = 366 nm; λ em = 610 nm for C3 and λ ex = 361 nm; λ em = 560 nm for C4). The TDDFT calculations suggest that an experimental mixed absorption band at 360 nm could be assigned to MLCT (d(Re) →π*(dmb))and LLCT (π(L)→π*(dmb)) transitions. We also assessed the cytotoxicity of C3 and C4 in an epithelial cell line (T84). We found that 12.5 µg/ml of C3 or C4 is the minimum concentration needed to kill the 80% of cell population, as determined by neutral red uptake. Finally, the potential of C3 and C4 as biological dyes for use in fluorescent microscopy was assessed in bacteria (Salmonella enterica) and yeasts (Candida albicans and Cryptococcus spp.), and in an ovarian cancer cell line (SKOV-3). We found that, in all cases, both C3 and C4 are suitable compounds to be used as fluorescent dyes for biological purposes. In addition, we present evidence suggesting that these rhenium (I) tricarbonyl complexes may be also useful as differential fluorescent dyes in yeasts (Candida albicans and Cryptococcus spp.), without the need of antibodies.

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Section: Introductionmentioning

confidence: 99%

Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO)₃⁺complexes with an electron withdrawing ancillary ligand

et al. 2016

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“…RNA-seq reads of N. pumilio (59) were merged and aligned to the draft genome of the species with STAR (60) to create a reference-based transcriptome using Scallop (61). Peptides were predicted with Transdecoder v5.5.0 (62). The predicted N. pumilio proteome was aligned using blastp (63) v2.11.0 against the reference annotated proteomes of Arabidopsis thaliana (64), Populus trichocarpa v3.0 (65) and Betula pendula (66) (parameters: -max_target_seqs 1 - outfmt 6 -evalue 1e-5).…”

Section: Methodsmentioning

confidence: 99%

Effect of temperature on circadian clock functioning of trees in the context of global warming

Estravis-Barcala,

Gaischuk,

Gonzalez-Polo

et al. 2024

Preprint

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Plant survival in a warmer world requires the timely adjustment of biological processes to cyclical changes in the new environment. Circadian oscillators have been proposed to contribute to thermal adaptation and plasticity in plants, due to their ability to maintain periodicity in biological rhythms over a wide temperature range, promoting fitness. However, the influence of temperature and circadian clock performance on plant behaviour in natural ecosystems is not well understood. Here we used two co-occurring Nothofagus tree species from the Patagonian forests that are adapted to contrasting thermal environments derived from their different altitudinal profiles. We revealed that the upper thermal limits for accurate clock function are linked to the species' thermal niches and contribute to seedling plasticity in natural environments. We computationally identified 24 circadian clock-related genes, which showed a high degree of structural conservation with clock genes from both annual and perennial species, and very similar patterns of gene expression to those of Arabidopsis thaliana. Warm temperatures produced a strong transcriptomic rearrangement, which affected the expression of clock-related genes and direct clock targets, evidencing the extent of clock functioning disruption by temperature. N. pumilio, the species from colder environments, showed reduced ability to keep rhythmicity at high temperatures compared to N. obliqua, which inhabits warmer zones. Accordingly, N. pumilio, but not N. obliqua, showed a limited oscillator function in warmer zones of the forest, reduced survival, and growth. Together, our results highlight the potential role of a resonating oscillator in ecological adaptation to a warming environment.

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“…The individual transcriptomes were then compared to the joint transcriptome to determine expression profiles. For identification of glycosyltransferases, Transdecoder (Haas & Papanicolaou, 2018) was used to identify possible protein sequences of at least 100 amino acids. The sequences were then analyzed using Interproscan (Jones et al, 2014) to identify characteristics found among GO terms, Pfam, PROSITE, and other categories for motifs.…”

Section: De Novo Transcriptome Assembly and Analysis For Glycosyltran...mentioning

confidence: 99%

Evaluation of pathways to the C‐glycosyl isoflavone puerarin in roots of kudzu (Pueraria montana lobata)

et al. 2022

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Kudzu (Pueraria montana lobata) is used as a traditional medicine in China and Southeast Asia but is a noxious weed in the Southeastern United States. It produces both O-and C-glycosylated isoflavones, with puerarin (C-glucosyl daidzein) as an important bioactive compound. Currently, the stage of the isoflavone pathway at which the C-glycosyl unit is added remains unclear, with a recent report of direct C-glycosylation of daidzein contradicting earlier labeling studies supporting C-glycosylation at the level of chalcone. We have employed comparative mRNA sequencing of the roots from two Pueraria species, one of which produces puerarin (field collected P. montana lobata) and one of which does not (commercial Pueraria phaseoloides), to identify candidate uridine diphosphate glycosyltransferase (UGT) enzymes involved in puerarin biosynthesis. Expression of recombinant UGTs in Escherichia coli and candidate C-glycosyltransferases in Medicago truncatula were used to explore substrate specificities, and gene silencing of UGT and key isoflavone biosynthetic genes in kudzu hairy roots employed to test hypotheses concerning the substrate(s) for C-glycosylation. Our results confirm UGT71T5 as a C-glycosyltransferase of isoflavone biosynthesis in kudzu. Enzymatic, isotope labeling, and genetic analyses suggest that puerarin arises both from the direct action of UGT71T5 on daidzein and via a second route in which the C-glycosidic linkage is introduced to the chalcone isoliquiritigenin. | INTRODUCTIONKudzu, Pueraria montana lobata, is a creeping, climbing perennial vine.With its diversity, adaptation, and rapid growth rate, kudzu has become an invasive species in the Southeastern United States (EPPO, 2007;Jewett et al., 2003;Sun et al., 2005). It has been planted on over 13,700 ha for erosion control in the Southeastern United States, but its replacement of existing vegetation results in ecological and economic losses, with lost productivity being estimated at $118 ha À1 year À1 (Britton et al., 2002;Sun et al., 2005).Kudzu is classified in the legume family Fabaceae and belongs to the Glycininae subtribe, which also includes soybean (Glycine max) (Wong et al., 2011). The classification of various kudzu varieties is imprecise, with multiple scientific names being ascribed to the same plant. The morphology of these plants can be very different. However, characteristics such as leaf morphology can also vary on the same

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Kinderkardiologie

Cited by 5 publications

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Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO)₃⁺complexes with an electron withdrawing ancillary ligand

Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO)₃⁺complexes with an electron withdrawing ancillary ligand

Effect of temperature on circadian clock functioning of trees in the context of global warming

Evaluation of pathways to the C‐glycosyl isoflavone puerarin in roots of kudzu (Pueraria montana lobata)

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Kinderkardiologie

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Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO)3+complexes with an electron withdrawing ancillary ligand

Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO)3+complexes with an electron withdrawing ancillary ligand

Effect of temperature on circadian clock functioning of trees in the context of global warming

Evaluation of pathways to the C‐glycosyl isoflavone puerarin in roots of kudzu (Pueraria montana lobata)

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Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO)₃⁺complexes with an electron withdrawing ancillary ligand

Fluorescence probes for prokaryotic and eukaryotic cells using Re(CO)₃⁺complexes with an electron withdrawing ancillary ligand