“…In contrast, isolate T016 from Yonsei University (BioProject PRJNA352855) (Song et al, 2017) is found here to be negative for all four trichomonasvirus spp. In this case, since the discrepancies involve a single trichomonasvirus species, the T. vaginalis isolate T016 from Yonsei University might have simply been "cured" of TVV2 during culture, as has been reported to occur in some other cases (Wang et al, 1987;Men-Fang et al, 1993).…”
Trichomonas vaginalis is the most common nonviral cause of sexually transmitted infections globally, with an estimated quarter of a billion people infected around the world. Infection by the protozoan parasite results in the clinical syndrome trichomoniasis, which manifests as an inflammatory syndrome with acute and chronic consequences. Half or more of these parasites are themselves infected with one or more dsRNA viruses which can exacerbate the inflammatory disease. Four distinct viruses have been found in T. vaginalis to date, Trichomonas vaginalis virus 1 through 4 (or TVVs). Despite the global prevalence of these viruses, few coding-complete genome sequences have been determined. We conducted viral sequence mining in publicly available transcriptomes across 60 RNA-seq datasets representing 13 distinct T. vaginalis isolates. We assembled sequences for 27 new trichomonasvirus strains across all known TVV species, with 17 of these assemblies representing coding-complete genomes. Using a strategy of de novo sequence assembly followed by taxonomic classification, we discovered a fifth species of TVV that we term Trichomonas vaginalis virus 5 (TVV5). Six strains of TVV5 were assembled, including two coding-complete genomes. These TVV5 sequences exhibit high sequence identity to each other, but low identity to any strains of TVV1-4. Phylogenetic analysis corroborates the species-level designation. These results substantially increase the number of coding-complete TVV genome sequences and demonstrate the utility of mining publicly available transcriptomes for the discovery of RNA viruses in a critical human pathogen.
“…In contrast, isolate T016 from Yonsei University (BioProject PRJNA352855) (Song et al, 2017) is found here to be negative for all four trichomonasvirus spp. In this case, since the discrepancies involve a single trichomonasvirus species, the T. vaginalis isolate T016 from Yonsei University might have simply been "cured" of TVV2 during culture, as has been reported to occur in some other cases (Wang et al, 1987;Men-Fang et al, 1993).…”
Trichomonas vaginalis is the most common nonviral cause of sexually transmitted infections globally, with an estimated quarter of a billion people infected around the world. Infection by the protozoan parasite results in the clinical syndrome trichomoniasis, which manifests as an inflammatory syndrome with acute and chronic consequences. Half or more of these parasites are themselves infected with one or more dsRNA viruses which can exacerbate the inflammatory disease. Four distinct viruses have been found in T. vaginalis to date, Trichomonas vaginalis virus 1 through 4 (or TVVs). Despite the global prevalence of these viruses, few coding-complete genome sequences have been determined. We conducted viral sequence mining in publicly available transcriptomes across 60 RNA-seq datasets representing 13 distinct T. vaginalis isolates. We assembled sequences for 27 new trichomonasvirus strains across all known TVV species, with 17 of these assemblies representing coding-complete genomes. Using a strategy of de novo sequence assembly followed by taxonomic classification, we discovered a fifth species of TVV that we term Trichomonas vaginalis virus 5 (TVV5). Six strains of TVV5 were assembled, including two coding-complete genomes. These TVV5 sequences exhibit high sequence identity to each other, but low identity to any strains of TVV1-4. Phylogenetic analysis corroborates the species-level designation. These results substantially increase the number of coding-complete TVV genome sequences and demonstrate the utility of mining publicly available transcriptomes for the discovery of RNA viruses in a critical human pathogen.
“…In contrast, isolate T016 from Yonsei University (BioProject PRJNA352855) [ 32 ] is found to be negative for all five trichomonasvirus species. In this case, because the discrepancies involve the presence or absence of a single TVV strain, the T. vaginalis isolate T016 from Yonsei University might have simply been cured of TVV2 during culture, as has been reported to occur in other cases [ 47 , 48 ].…”
Trichomonas vaginalis is the most common non-viral cause of sexually transmitted infections globally. Infection by this protozoan parasite results in the clinical syndrome trichomoniasis, which manifests as an inflammatory disease with acute and chronic consequences. Half or more isolates of this parasite are themselves infected with one or more dsRNA viruses that can exacerbate the inflammatory syndrome. At least four distinct viruses have been identified in T. vaginalis to date, constituting species Trichomonas vaginalis virus 1 through Trichomonas vaginalis virus 4 in genus Trichomonasvirus. Despite the global prevalence of these viruses, few complete coding sequences have been reported. We conducted viral sequence mining in publicly available transcriptomes across 60 RNA-Seq accessions representing at least 13 distinct T. vaginalis isolates. The results led to sequence assemblies for 27 novel trichomonasvirus strains across all four recognized species. Using a strategy of de novo sequence assembly followed by taxonomic classification, we additionally discovered six strains of a newly identified fifth species, for which we propose the name Trichomonas vaginalis virus 5, also in genus Trichomonasvirus. These additional strains exhibit high sequence identity to each other, but low sequence identity to strains of the other four species. Phylogenetic analyses corroborate the species-level designations. These results substantially increase the number of trichomonasvirus genome sequences and demonstrate the utility of mining publicly available transcriptomes for virus discovery in a critical human pathogen.
“…6-The tube which contains DNA was stored at -20Cº till used for amplification. 13), PCR Identification of Trichomonas vaginalis according procedure by as (14) with some modifications and products analysis according procedure used by (13) as seen in figure (1).…”
The aim of this research was to diagnosis of Trichomonas vaginalis by use PCR technique and evaluate interleukin-6 (IL-6) in men infected with Trichomonas vaginalis; urine and blood specimens were collected from 85 men whom have visited the department of infertility at Al-Sadder medical city, Al-Zahra Hospital in Najaf Province during the period from June till October, 2012. Twenty healthy looking age matched men taken to serum tubes and serum was separated. Serum was used for evolution of the IL-6.The IL-6 was evaluated in serum using ELISA technique .Trichomonas vaginalis was isolated from 15 men with a prevalence rate 17.64% by using PCR technique. The results revealed a significant increase in IL-6 in men infected with Trichomonas vaginalis in compared to control group and the PCR is accurate method used in diagnosis of Trichomonas vaginalis parasite.
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