Killing of Cryptosporidium sporozoites by Lactoferrin

Paredes, José Luís; Sparks, Hayley; White, A. Clinton; Martinez-Traverso, Griselle; Ochoa, Theresa J.; Castellanos-González, Alejandro

doi:10.4269/ajtmh.16-0804

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…The authors showed possible inhibition of sporozoite viability and infectivity, but no effect was found on the excystation stage of the oocyst wall. These studies suggested that Lf protein or its peptides are impermeable to the thick wall of the oocyst but can inhibit the viability of free forms of this parasite through internalization via the endocytic pathway (Paredes et al, 2017).…”

Section: Antiparasitic Effect Of Lf and Its Peptides Against Cryptosp...mentioning

confidence: 99%

Antiparasitic activity of the iron-containing milk protein lactoferrin and its potential derivatives against human intestinal and blood parasites

Anand

2024

Front. Parasitol.

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An iron-containing milk protein named lactoferrin (Lf) has demonstrated antiparasitic and immunomodulatory properties against a variety of human parasites. This protein has shown its capability to bind and transport iron molecules in the vicinity of the host–pathogen environment. The ability of parasites to sequester the iron molecule and to increase their pathogenicity and survival depends on the availability of iron sources. Lf protein has suggested a iron chelating effect on parasites iron and, hence, has shown its antiparasitic effect. Since the parasites have a complex life cycle and have developed drug resistance, vaccines and other treatments are a handful. Therefore, therapeutic research focusing on natural treatment regimens that target the parasite and are non-toxic to host cells is urgently needed. The antiparasitic efficacy of Lf protein has been extensively studied over the past 40 years using both in vitro and in vivo studies. This review article highlighted past important studies on Lf protein that revealed its potential antiparasitic activity against various intracellular and extracellular intestinal or blood-borne human parasites. This review article structures the role of Lf protein in its various forms, such as native, peptide, and nanoformulation, laying the groundwork for its function as an antiparasitic agent and its possible known mechanisms of action.

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Section: Antiparasitic Effect Of Lf and Its Peptides Against Cryptosp...mentioning

confidence: 99%

Antiparasitic activity of the iron-containing milk protein lactoferrin and its potential derivatives against human intestinal and blood parasites

Anand

2024

Front. Parasitol.

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“…94 For example, LF has inhibitory activity against different stages of Cryptosporidium and can kill Cryptosporidium cysts as well as treat Cryptosporidium-related gastrointestinal infections. 95 Probiotics are an important group of beneficial gut microorganisms, and LF can regulate their formation, enrichment, and equilibrium. 96 The consumption of probiotics may contribute to the balance of beneficial microbiota in the gastrointestinal tract, and the property of LF to bind iron allows it to promote the growth of bif idobacteria and lactobacilli.…”

Section: Ferritinsmentioning

confidence: 99%

“…Some studies have combined LF with probiotics and demonstrated that LF inhibits the growth of particular pathogens, thus influencing the composition of the gut microbiota by inhibiting gut pathogens . For example, LF has inhibitory activity against different stages of Cryptosporidium and can kill Cryptosporidium cysts as well as treat Cryptosporidium-related gastrointestinal infections . Probiotics are an important group of beneficial gut microorganisms, and LF can regulate their formation, enrichment, and equilibrium .…”

Section: Lf Regulates Intestinal Inflammation Via the Gut Microbiotamentioning

confidence: 99%

Research Advances of Lactoferrin in Electrostatic Spinning, Nano Self-Assembly, and Immune and Gut Microbiota Regulation

Dong

et al. 2022

J. Agric. Food Chem.

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Lactoferrin (LF) is a naturally present iron-binding globulin with the structural properties of an N-lobe strongly positively charged terminus and a cage-like structure of nano self-assembly encapsulation. These unique structural properties give it potential for development in the fields of electrostatic spinning, targeted delivery systems, and the gut-brain axis. This review will provide an overview of LF’s unique structure, encapsulation, and targeted transport capabilities, as well as its applications in immunity and gut microbiota regulation. First, the microstructure of LF is summarized and compared with its homologous ferritin, revealing both structural and functional similarities and differences between them. Second, the electrostatic interactions of LF and its application in electrostatic spinning are summarized. Its positive charge properties can be applied to functional environmental protection packaging materials and to improving drug stability and antiviral effects, while electrostatic spinning can promote bone regeneration and anti-inflammatory effects. Then the nano self-assembly behavior of LF is exploited as a cage-like protein to encapsulate bioactive substances to construct functional targeted delivery systems for applications such as contrast agents, antibacterial dressings, anti-cancer therapy, and gene delivery. In addition, some covalent and noncovalent interactions of LF in the Maillard reaction and protein interactions and other topics are briefly discussed. Finally, LF may affect immunological function via controlling the gut microbiota. In conclusion, this paper reviews the research advances of LF in electrostatic spinning, nano self-assembly, and immune and gut microbiota regulation, aiming to provide a reference for its application in the food and pharmaceutical fields.

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“…The gardicidal effect of lactoferrin and lactoferrin-derived peptides was also observed at low concentrations, where they caused dilation of the endoplasmic reticulum (ER) membranes, expansion of the nuclear membrane, and plasma membrane protrusions [ 211 ], although high concentrations cause severe morphological changes or even induce programmed cell death [ 212 , 213 ]. Lactoferrin also exhibited antiparasitic activity against Cryptosporidium parvum sporozoites but had no significant effect on oocysts viability or parasite intracellular development [ 214 ].…”

Section: Antiparasitic Activities Of Lactoferrins and Lactoferrin-derived Peptidesmentioning

confidence: 99%

Diverse Mechanisms of Antimicrobial Activities of Lactoferrins, Lactoferricins, and Other Lactoferrin-Derived Peptides

Gruden

Ulrih

2021

IJMS

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Lactoferrins are an iron-binding glycoprotein that have important protective roles in the mammalian body through their numerous functions, which include antimicrobial, antitumor, anti-inflammatory, immunomodulatory, and antioxidant activities. Among these, their antimicrobial activity has been the most studied, although the mechanism behind antimicrobial activities remains to be elucidated. Thirty years ago, the first lactoferrin-derived peptide was isolated and showed higher antimicrobial activity than the native lactoferrin lactoferricin. Since then, numerous studies have investigated the antimicrobial potencies of lactoferrins, lactoferricins, and other lactoferrin-derived peptides to better understand their antimicrobial activities at the molecular level. This review defines the current antibacterial, antiviral, antifungal, and antiparasitic activities of lactoferrins, lactoferricins, and lactoferrin-derived peptides. The primary focus is on their different mechanisms of activity against bacteria, viruses, fungi, and parasites. The role of their structure, amino-acid composition, conformation, charge, hydrophobicity, and other factors that affect their mechanisms of antimicrobial activity are also reviewed.

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Killing of Cryptosporidium sporozoites by Lactoferrin

Cited by 7 publications

References 18 publications

Antiparasitic activity of the iron-containing milk protein lactoferrin and its potential derivatives against human intestinal and blood parasites

Antiparasitic activity of the iron-containing milk protein lactoferrin and its potential derivatives against human intestinal and blood parasites

Research Advances of Lactoferrin in Electrostatic Spinning, Nano Self-Assembly, and Immune and Gut Microbiota Regulation

Diverse Mechanisms of Antimicrobial Activities of Lactoferrins, Lactoferricins, and Other Lactoferrin-Derived Peptides

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