Killer character in<i>Kluyveromyces</i>yeasts: Activity on<i>Kloeckera apiculata</i>

Rosini, Gianfranco; Cantini, Mauro

doi:10.1111/j.1574-6968.1987.tb02247.x

Cited by 18 publications

(6 citation statements)

References 23 publications

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“…Previous studies have reported a broad spectrum of activity for Kpkt, and demonstrated its stability under winemaking conditions, indicating its promising features for use as a novel antiseptic agent in the wine and beverage industries [10,18,27]. In the present study, the mode of action Kpkt was characterized in view of its potential application as a natural antimicrobial agent.…”

Section: Discussionmentioning

confidence: 79%

Tetrapisispora phaffii killer toxin is a highly specific β-glucanase that disrupts the integrity of the yeast cell wall

2009

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BackgroundKiller yeasts have been used to combat contaminating wild yeasts in food, to control pathogenic fungi in plants, and in the medical field, to develop novel antimycotics for the treatment of human and animal fungal infections. Among these killer yeasts, Tetrapisispora phaffii (formerly known as Kluyveromyces phaffii) secretes a glycoprotein known as Kpkt that is lethal to spoilage yeasts under winemaking conditions. In the present study, the mode of action of Kpkt, and the specific damage produced by this toxin on sensitive yeasts is investigated.ResultsThe use of castanospermine, a β-glucanase inhibitor, demonstrated that β-glucanase activity is essential for the Kpkt killer activity in vivo. Accordingly, Kpkt has no killer activity on either sensitive yeast spheroplasts or whole sensitive cells in the presence of isosmothic medium (0.8 molar sorbitol). Kpkt induces ultrastructural modifications in the cell wall of sensitive strains, as shown by confocal microscopy, laser-scanning electron microscopy, and atomic force microscopy. The Kpkt killer action is mediated by the glucidic portion of the toxin. This, in turn, appears to be involved both in the stronger cytocidal activity and in the selectivity for the sensitive strain shown by Kpkt compared to laminarinase.ConclusionCollectively, these data indicate that the mode of action of Kpkt is directed towards the disruption of cell-wall integrity, and that this is mediated by a highly specific β-glucanase activity. In this, Kpkt differs from other microbial β-glucanases that do not show killer activities.

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Section: Discussionmentioning

confidence: 79%

Tetrapisispora phaffii killer toxin is a highly specific β-glucanase that disrupts the integrity of the yeast cell wall

2009

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“…Somewhat later, other researchers reported the antagonistic action of yeasts against other yeasts, involving the production of secondary metabolites known as killer toxins or “mycocins” (Young and Yagiu, 1978; Rosini and Cantini, 1987; Walker et al, 1995; Suzuki et al, 2001; Marquina et al, 2002). In summarizing the antimicrobial effects of yeasts present in fermented foods and beverages, Viljoen (2006) mentions actions of organic acids, antibiotic factors, volatile acids, hydrogen peroxide, and various other substrates excreted in the product.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Probiotic Properties of Yeasts: From Fundamental to Novel Applications

Labrie²,

2012

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The yeasts constitute a large and heterogeneous group of microorganisms that are currently attracting increased attention from scientists and industry. Numerous and diverse biological activities make them promising candidates for a wide range of applications not limited to the food sector. In addition to their major contribution to flavor development in fermented foods, their antagonistic activities toward undesirable bacteria, and fungi are now widely known. These activities are associated with their competitiveness for nutrients, acidification of their growth medium, their tolerance of high concentrations of ethanol, and release of antimicrobial compounds such as antifungal killer toxins or “mycocins” and antibacterial compounds. While the design of foods containing probiotics (microorganisms that confer health benefits) has focused primarily on Lactobacillus and Bifidobacterium, the yeast Saccharomyces cerevisiae var. boulardii has long been known effective for treating gastroenteritis. In this review, the antimicrobial activities of yeasts are examined. Mechanisms underlying this antagonistic activity as well as recent applications of these biologically active yeasts in both the medical and veterinary sectors are described.

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“…K . wickerhamii CCY 21-41-1 is the same strain as that used by ROSINI and CANTINI [17] named K . wickenii U.C.D.…”

Section: Resultsmentioning

confidence: 99%

Killer yeasts of Kluyveromyces and Hansenula genera with potential application in fermentation and therapy

Michalčáková

Sulo

Sláviková

1993

Acta Biotechnologica

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The killing/immunity interactions among killer strains of the genera Kluyveromyces, Hansenula and Saccharomyces from the Czechoslovak Collection. of Yeasts were studied with the aim to find the strains with broad specificity and killer activity targeted against a range of undesirable wild yeasts causing stuck fermentations. Among 49 tested Kluyveromyces strains, five strains were found, and among 55 Hansenula strains, ten yeast strains were found with activity against a sensitive strain of Saccharomyces. Hansenula mrakii CCY 38-7-I and Hansenula saturnus var. subsufficiens CCY 38-4-2 showed exceptional activity against the wine contaminants, Zygosaccharomyces builii, as well as against pathogenic Candida species within a broad range of pH 2.9 -5.1. Their potential biotechnological application is discussed.

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Killer character inKluyveromycesyeasts: Activity onKloeckera apiculata

Cited by 18 publications

References 23 publications

Tetrapisispora phaffii killer toxin is a highly specific β-glucanase that disrupts the integrity of the yeast cell wall

Tetrapisispora phaffii killer toxin is a highly specific β-glucanase that disrupts the integrity of the yeast cell wall

Antimicrobial and Probiotic Properties of Yeasts: From Fundamental to Novel Applications

Killer yeasts of Kluyveromyces and Hansenula genera with potential application in fermentation and therapy

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