KIF2A silencing inhibits the proliferation and migration of breast cancer cells and correlates with unfavorable prognosis in breast cancer

Wang, JianLi; Ma, Siqin; Ma, Rong; Qu, Xun; Chen, Li; Lv, Cuixia; Zhao, Song; Gong, Yun

doi:10.1186/1471-2407-14-461

Cited by 102 publications

(140 citation statements)

References 29 publications

Supporting

Mentioning

135

Contrasting

Order By: Relevance

“…Function in positioning lysosomes has been described (21), although the molecular mechanism was not examined. More recently, a connection to cell migration has been established (22,23). The effect could be mediated by control of focal adhesions (FAs).…”

Section: Discussionmentioning

confidence: 99%

“…Kif2 has been reported to affect lysosomal positioning (21). Increased Kif2A expression levels have also been reported to be an indicator of poor prognosis and higher metastatic potential, and to contribute to cell migration and invasion in vitro in squamous cell carcinoma and breast cancer (22,23). In transformed bronchial epithelial cells, Kif2A and Kif2C levels are regulated by K-Ras and contribute to the invasive behavior of the cells, but not to epithelial-to-mesenchymal transition (24).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Direct Functional Interaction of the Kinesin-13 Family Membrane Kinesin-like Protein 2A (Kif2A) and Arf GAP with GTP-binding Protein-like, Ankyrin Repeats and PH Domains1 (AGAP1)

Luo¹,

Chen

Wagenbach

et al. 2016

Journal of Biological Chemistry

View full text Add to dashboard Cite

The molecular basis for control of the cytoskeleton by the Arf GTPase-activating protein AGAP1 has not been characterized. AGAP1 is composed of G-protein-like (GLD), pleckstrin homology (PH), Arf GAP, and ankyrin repeat domains. Kif2A was identified in screens for proteins that bind to AGAP1. The GLD and PH domains of AGAP1 bound the motor domain of Kif2A. Kif2A increased GAP activity of AGAP1, and a protein composed of the GLD and PH domains of AGAP1 increased ATPase activity of Kif2A. Knockdown (KD) of Kif2A or AGAP1 slowed cell migration and accelerated cell spreading. The effect of Kif2A KD on spreading could be rescued by expression of Kif2A-GFP or FLAG-AGAP1, but not by Kif2C-GFP. The effect of AGAP1 KD could be rescued by FLAG-AGAP1, but not by an AGAP1 mutant that did not bind Kif2A efficiently, Arf-GAP1-HA or Kif2A-GFP. Taken together, the results support the hypothesis that the Kif2A⅐AGAP1 complex contributes to control of cytoskeleton remodeling involved in cell movement.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Direct Functional Interaction of the Kinesin-13 Family Membrane Kinesin-like Protein 2A (Kif2A) and Arf GAP with GTP-binding Protein-like, Ankyrin Repeats and PH Domains1 (AGAP1)

Luo¹,

Chen

Wagenbach

et al. 2016

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Furthermore, it was demonstrated that KIF2A overexpression was associated with tumorigenesis and cancer progression and indicative of poor prognosis. For example, abnormal KIF2A expression was associated with metastasis and poor prognosis of breast cancer [12], glioma [13] and gastric cancer [14]. In our pervious study, we also found that KIF2A was also associated with ovarian cancer [15].…”

Section: Introductionmentioning

confidence: 49%

“…KIF2A, a type of motor protein with an oncogenic role, is overexpressed in many human malignancies [12-15]. KIF2A is associated with the development and progression of various human cancers and can be an indicator of poor prognosis.…”

Section: Discussionmentioning

confidence: 99%

“…The previous studies have been reported that the proliferation and migration of cancers cells were promoted and apoptosis were inhibited by high expression of KIF2A which predicted poor prognosis in several cancers, such as in breast cancer [12], glioma [13], squamous cell carcinoma of the oral tongue [27] and head and neck squamous cell carcinoma [28]. Additionally, recent studies have also shown that miR-206, a tumor suppressor miRNA that targets several oncogenes, was under expressed in many tumors [16-20], miR-206 inhibited cell proliferation, invasion and/or apoptosis by targeting SOX9 in lung cancer [16], Tbx3 in breast cancer [18], CDK9 in hepatocellular carcinoma [19], HDAC6in head and neck squamous cell carcinoma [20], ANXA2 in osteosarcoma [21], Bcl-2 in colorectal cancer [29], CXCL11 in prostate cancer [30], HDAC6 in squamous cell carcinoma [27], and cell metastasis by targeting MKL1/IL11 in breast cancer [17], FMNL2 in colorectal cancer [31], CXCL11 in prostate cancer [30].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of KIF2A is Suppressed by miR-206 and Associated with Poor Prognosis in Ovarian Cancer

Sheng¹,

Xu²,

Xi³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: This study aimed to investigate the expression and prognostic value of kinesin family member 2A (KIF2A) and the suppression effects of microRNA-206 (miR-206) on KIF2A in ovarian cancer. Methods: Ovarian cancer tissues from patients and ovarian cancer cell lines (A2780 and SKOV3) were used in this study. miR-206 mimics and control were transiently transfected into cells. RT-qPCR was performed to detect KIF2A mRNA and miR-206 expression levels, Western blot was performed to detect KIF2A protein levels, Dual-Luciferase Reporter Assay was used to examine the inhibition effects of miR-206 on KIF2A mRNA, immunohistochemical staining was used to examine the expression of KIF2A in tissue sections. CCK-8, transwell and Annexin-V-FITC/Propidium Iodide staining with flow cytometry were used to detect the cell proliferation, migration/invasion, and apoptosis respectively. Results: Our study explored the expression profiles of KIF2A and miR-206 in the patients with ovarian cancer. We found that overexpression of KIF2A was associated with a poor prognosis in ovarian cancer. We also found that KIF2A mRNA contains two target sites for miR-206 binding and confirmed that miR-206 directly suppresses KIF2A; inhibits ovarian cancer cell proliferation, migration, and invasion; and induces apoptosis. Conclusion: The results suggest KIF2A could serve a valuable prognostic indicator in ovarian cancer and provide a rationale for treatment of ovarian cancer by targeting KIF2A via miR-206.

show abstract

Prognostic biomarkers correlated with immune infiltration in non‐small cell lung cancer

Cui

Liu

et al. 2022

FEBS Open Bio

View full text Add to dashboard Cite

Lung cancer is the leading cause of cancer‐related mortality in men and women globally. Non‐small cell lung cancer (NSCLC) is the most prevalent subtype, accounting for 85–90% of all cancers. Although there have been dramatic advances in therapeutic approaches in recent decades, the recurrence and metastasis rates of NSCLC are as high as 30–40% with the 5‐year overall survival rate being less than 15%. Therefore, it is necessary to explore the pathogenesis of NSCLC at the genetic level and identify prognostic biomarkers and novel therapeutic targets. Here, we aimed to identify mutated genes with high frequencies in Chinese NSCLC patients using next‐generation sequencing and to investigate their relationships with the tumor mutation burden (TMB) and tumor immune microenvironment. A total of 110 NSCLC patients were enrolled to profile the genetic variations. Mutations in EGFR (62.37%), TP53 (61.29%), LRP1B (13.98%), FAT1 (12.90%), KMT2D (11.83%), CREBBP (10.75%), and RB1 (9.68%) were most prevalent. TP53, LRP1B, KMT2D, and CREBBP mutations were all significantly associated with high TMB (P < 0.05 or P < 0.01). The infiltrating levels of immune cells and immune molecules were enriched significantly in the LRP1B mutation group. LRP1B mutations significantly correlated with stimulating and inhibitory immunoregulators. Gene set enrichment analysis revealed that cell cycle, the Notch signaling pathway, the insulin signaling pathway, and the mTOR signaling pathway are related to LRP1B mutations in the immune system. LRP1B mutations may be of clinical importance in enhancing the anti‐tumor immune response and may be a promising biomarker for predicting immunotherapy responsiveness.

show abstract

KIF2A silencing inhibits the proliferation and migration of breast cancer cells and correlates with unfavorable prognosis in breast cancer

Cited by 102 publications

References 29 publications

Direct Functional Interaction of the Kinesin-13 Family Membrane Kinesin-like Protein 2A (Kif2A) and Arf GAP with GTP-binding Protein-like, Ankyrin Repeats and PH Domains1 (AGAP1)

Direct Functional Interaction of the Kinesin-13 Family Membrane Kinesin-like Protein 2A (Kif2A) and Arf GAP with GTP-binding Protein-like, Ankyrin Repeats and PH Domains1 (AGAP1)

Overexpression of KIF2A is Suppressed by miR-206 and Associated with Poor Prognosis in Ovarian Cancer

Prognostic biomarkers correlated with immune infiltration in non‐small cell lung cancer

Contact Info

Product

Resources

About