KIF17 stabilizes microtubules and contributes to epithelial morphogenesis by acting at MT plus ends with EB1 and APC

Jaulin, Fanny; Kreitzer, Geri

doi:10.1083/jcb.201006044

Cited by 91 publications

(140 citation statements)

References 88 publications

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“…However, apart from a general subapical concentration of microtubules, we did not observe that detyrosinated or tyrosinated microtubules were specifically focused to one of the two cell poles. A predominantly subapical accumulation could be detected for acetylated tubulin as previously shown (Jaulin and Kreitzer, 2010). Therefore, we propose that additional factors assist in intracellular guidance to the specific membrane domains of these cells.…”

Section: Discussionsupporting

confidence: 81%

“…1A; see also supplementary material Fig. S1), which supports data from Jaulin and Kreitzer who showed an enrichment of acetylated tubulin at the apical cell pole (Jaulin and Kreitzer, 2010). In a further study, the quantity of detyr-and tyr-tubulin at the two cell poles was studied by TIRF microscopy, which detects fluorescent dyes in close proximity to the apical or basal plasma membrane of polarized MDCK cells (Schneider et al, 2010).…”

Section: Distribution Of Detyrosinated and Tyrosinated Tubulin In Polsupporting

confidence: 83%

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Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells

Zink

Große

Freikamp

et al. 2012

Journal of Cell Science

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SummaryThe role of post-translational tubulin modifications in the development and maintenance of a polarized epithelium is not well understood. We studied the balance between detyrosinated (detyr-) and tyrosinated (tyr-) tubulin in the formation of MDCK cell monolayers. Increased quantities of detyrosinated microtubules were detected during assembly into confluent cell sheets. These tubules were composed of alternating stretches of detyr-and tyr-tubulin. Constant induction of tubulin tyrosination, which decreased the levels of detyr-tubulin by overexpression of tubulin tyrosine ligase (TTL), disrupted monolayer establishment. Detyr-tubulin-depleted cells assembled into isolated islands and developed a prematurely polarized architecture. Thus, tubulin detyrosination is required for the morphological differentiation from non-polarized cells into an epithelial monolayer. Moreover, membrane trafficking, in particular to the apical domain, was slowed down in TTL-overexpressing cells. This effect could be reversed by TTL knockdown, which suggests that detyr-tubulin-enriched microtubules serve as cytoskeletal tracks to guide membrane cargo in polarized MDCK cells.

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Section: Discussionsupporting

confidence: 81%

Section: Distribution Of Detyrosinated and Tyrosinated Tubulin In Polsupporting

confidence: 83%

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells

Zink

Große

Freikamp

et al. 2012

Journal of Cell Science

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“…Accordingly, our data can be interpreted as showing preferential recruitment and increased growth rate of MTs at cell-extracellular-matrix contacts versus cell-cell contacts, in relation to the establishment of a front-rear cell polarity required for migration (Kadir et al, 2011). These data are consistent with previous observations of a clear bias of MT growth towards the free edge of cells at the border of cell islands (Dupin et al, 2009;Jaulin and Kreitzer, 2010). Thus, our observations are consistent with a major role for a balance between spatially segregated integrin and N-cadherin signaling in biasing MT distribution and dynamics.…”

Section: Discussionsupporting

confidence: 82%

Adhesive interactions of N-cadherin limit the recruitment of microtubules to cell–cell contacts through organization of actomyosin

Plestant¹,

Strale²,

Seddiki³

et al. 2014

Development

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Adhesive interactions of cadherins induce crosstalk between adhesion complexes and the actin cytoskeleton, allowing strengthening of adhesions and cytoskeletal organization. The underlying mechanisms are not completely understood, and microtubules (MTs) might be involved, as for integrin-mediated cell-extracellular-matrix adhesions. Therefore, we investigated the relationship between N-cadherin and MTs by analyzing the influence of N-cadherin engagement on MT distribution and dynamics. MTs progressed less, with a lower elongation rate, towards cadherin adhesions than towards focal adhesions. Increased actin treadmilling and the presence of an actomyosin contractile belt, suggested that actin relays inhibitory signals from cadherin adhesions to MTs. The reduced rate of MT elongation, associated with reduced recruitment of end-binding (EB) proteins to plus ends, was alleviated by expression of truncated N-cadherin, but was only moderately affected when actomyosin was disrupted. By contrast, destabilizing actomyosin fibers allowed MTs to enter the adhesion area, suggesting that tangential actin bundles impede MT growth independently of MT dynamics. Blocking MT penetration into the adhesion area strengthened cadherin adhesions. Taken together, these results establish a crosstalk between N-cadherin, Factin and MTs. The opposing effects of cadherin and integrin engagement on actin organization and MT distribution might induce bias of the MT network during cell polarization.

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“…APC promotes persistent growth of microtubule ends and guides them to the front of the lamellipodia [Näthke et al, 1996;Kita et al, 2006]. APC remains attached to the extending microtubule ends with the help of plus end-directed motor activities [MimoriKiyosue et al, 2000;Jimbo et al, 2002;Jaulin and Kreitzer, 2010], which results in accumulation of APC at the ends of pioneering microtubules (Fig. 3A-b).…”

Section: Directional Migrationmentioning

confidence: 99%

Shaping microtubules into diverse patterns: Molecular connections for setting up both ends

Mimori-Kiyosue¹

2011

Cytoskeleton

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Microtubules serve as rails for intracellular trafficking and their appropriate organization is critical for the generation of cell polarity, which is a foundation of cell differentiation, tissue morphogenesis, ontogenesis and the maintenance of homeostasis. The microtubule array is not just a static railway network; it undergoes repeated collapse and reassembly in diverse patterns during cell morphogenesis. In the last decade much progress has been made toward understanding the molecular mechanisms governing complex microtubule patterning. This review first revisits the basic principle of microtubule dynamics, and then provides an overview of how microtubules are arranged in highly shaped and functional patterns in cells changing their morphology by factors controlling the fate of microtubule ends. V C 2011 Wiley Periodicals, Inc.

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KIF17 stabilizes microtubules and contributes to epithelial morphogenesis by acting at MT plus ends with EB1 and APC

Abstract: Cell polarity is determined in part by Kif17-mediated regulation of microtubule dynamics and polymerization rates.

Cited by 91 publications

References 88 publications

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells

Adhesive interactions of N-cadherin limit the recruitment of microtubules to cell–cell contacts through organization of actomyosin

Shaping microtubules into diverse patterns: Molecular connections for setting up both ends

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