Kielbone® in healing interradicular lesions in monkeys

Abstract

The purpose of this investigation was, to compare the histological features of Kielbone and autogenous jaw bone following transplantation into periodontal interradicular lesions in monkeys. Bifurcation defects were produced on mandibular molars and premolars. Kielbone was transplanted into 40 bifurcations while another 30 bifurcations received fresh autogenous cancellous jaw bone. The animals were sacrificed according to a schedule yielding observation periods up to 16 weeks. Before sacrifice the animals were perfused with a mixture of india ink and serum (3:1) by cannulization of the carotid arteries. The specimens were decalcified in a formic acid/sodium citrate solution. Cleared specimens 100μ of thickness were prepared from one half of each specimen. The other half of the specimen was cut into serial sections 8μ thick and stained with hematoxylin and eosin or Mallory's connective tissue stain. During healing of the treated defects Kielbone grafts and grafts of fresh autogenous bone from the jaws displayed similar histological features. In the bifurcation defects, Kielbone as well as autogenous bone grafts occured as isolated bone particles surrounded by a cementum‐like substance. It is suggested that Kielbone may constitute a suitable substitute for autogenous bone grafts in the treatment of periodontal intrabony defects. However, it should be emphasized that neither autogenous jaw bone nor Kielbone did favour bone regeneration in the bifurcation defects.

Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral

“…It is well known that intimate interactions occur between collagens and biological apatites in vivo. 20,40 These observations, in conjunction with our previous studies, 24 indicate that this three-dimensional culture system may provide osteoblast-like cells with an environment more similar to in vivo conditions than the collagen lattice gel 23 or the monolayer culture.…”

A comparative study on the use of a HA/collagen/chondroitin sulphate biomaterial (Biostite®) and a bovine‐derived HA xenograft (Bio‐Oss®) in the treatment of deep intra‐osseous defects

“…Clinical (Scopp et al 1966, Older 1967) and histological (Arrocha et al 1968, Nielsen et al 1980, 1981 studies promoted the use of anorganic bovine bone for grafting in intra-osseous defects. In this context, an anorganic xenograft (Bio-Oss s , Geistlich Pharma AG, Wolhusen, Switzerland) was developed for bone regeneration procedures.…”

Impact of Stem Cells in Craniofacial Regenerative Medicine

“…The periodontal ligament suspends the tooth like a cushion in order transduce the mechanical load from the teeth evenly onto the supporting bone. Early studies of different animal models demonstrated that the periodontal tissues possess some regeneration activity, suggesting the existence of stem cell population within the periodontium (Karring et al, 1980; Nielsen et al, 1980; Nyman et al, 1980, 1982; Parlar et al, 2005). After depletion of various periodontal tissues, not only the periodontal ligaments, but also cementum and alveolar bone, can be regenerated, suggesting the presence of multipotential stem cell populations (Nielsen et al, 1980; Nyman et al, 1982; Parlar et al, 2005).…”

“…Early studies of different animal models demonstrated that the periodontal tissues possess some regeneration activity, suggesting the existence of stem cell population within the periodontium (Karring et al, 1980; Nielsen et al, 1980; Nyman et al, 1980, 1982; Parlar et al, 2005). After depletion of various periodontal tissues, not only the periodontal ligaments, but also cementum and alveolar bone, can be regenerated, suggesting the presence of multipotential stem cell populations (Nielsen et al, 1980; Nyman et al, 1982; Parlar et al, 2005). Studies conducted on the human periodontium indicate the presence of a putative PDLSCs population (Seo et al, 2004), positive for MSC markers including STRO-1 and CD146 and able to differentiate into osteoblasts, adipocytes, and cementoblasts (Seo et al, 2004).…”