Kidney-targeted liposome-mediated gene transfer in mice

Systemic administration of naked DNA with targeting specificity to mammalian kidneys

“…[41][42][43] In contrast, nonviral vectors consistently produce high gene expression either in the cortex or in the outer medulla and almost no expression could be detected in the inner medulla. 46 Lai et al 46 demonstrated a prolonged systemic gene expression in the kidney after intravenous delivery of DNA-liposome complex in mice. Although the level of exogenous gene expression in the kidney is relatively low, it was mostly restricted to the endothelium.…”

“…Although the level of exogenous gene expression in the kidney is relatively low, it was mostly restricted to the endothelium. 46 Lai et al 46 reported kidney-targeted gene delivery in mice using DOTMA-DOPE via intrarenal-pelvic or intrarenal-arterial injections. Gene expression was, however, mostly confined to the outer medulla.…”

“…Gene expression was, however, mostly confined to the outer medulla. 46 Although the mechanism of DNA uptake mediated by nonviral gene delivery systems is not well characterized, it is possible that differences in the microenvironment between the inner and outer medulla would affect the interactions between the internalization of the exogenous DNA and the target cells for viral and nonviral vectors. Such differences in interactions at the local microenvironment may promote or prevent DNA uptake by the different target cells and could result in differences in gene expression.…”

Systemic administration of naked plasmid encoding hepatocyte growth factor ameliorates chronic renal fibrosis in mice

“…Second, the injection procedure itself is simple and convenient without involvement of any surgical procedures as seen by renal artery and retrograde uerteric infusions. 25,31 Third, delivery of plasmid vector, as well as HGF expression is proven to be safe in vivo. 20 Fourth, the duration of HGF expression in vivo is controllable via repeated administrations.…”

Glomerular filtration is required for transfection of proximal tubular cells in the rat kidney following injection of DNA complexes into the renal artery

“…6 In these cases, access to the kidney was achieved via direct injection into the left renal artery, but intrapelvic injections have also been employed, leading to transgene expression in the papilla and medulla, 3 or in the outer medulla. 7 In our previous work, we showed that DNA complexed with branched 25 kDa PEI at 10 equivalents (eq) of amino to phosphate (N/P) groups is able to transfect proximal tubular cells following injection into the rat renal artery. Conversely, transfection efficiency of DNA complexed to 5 N/P eq of PEI, or to cationic lipids such as DOTAP, was significantly lower.…”