Kidney-resident macrophages promote a proangiogenic environment in the normal and chronically ischemic mouse kidney

Puranik, Amrutesh S.; Leaf, Irina A.; Jensen, Mark A.; Hedayat, Ahmad F.; Saad, Ahmed; Kim, Ki Wook; Saadalla, Abdulrahman; Woollard, John R.; Kashyap, Sonu; Textor, Stephen C.; Grande, Joseph P.; Lerman, Amir; Simari, Robert D.; Randolph, Gwendalyn J.; Duffield, Jeremy S.; Lerman, Lilach O.

doi:10.1038/s41598-018-31887-4

Cited by 84 publications

(80 citation statements)

References 66 publications

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“…These included major early and late injury-induced changes in kidney-resident macrophages and T cell populations. The changes in macrophage subtypes from a tissue-resident to monocyte-derived population following AKI has also been described by others (48,49). Interestingly, we saw a major contribution of the T cell response and a highly dynamic adaptation of this population during injury, regeneration, and fibrosis.…”

Section: Discussionsupporting

confidence: 84%

Immune cell landscaping reveals a protective role for regulatory T cells during kidney injury and fibrosis

Durães¹,

Lafont²,

Beibel³

et al. 2020

JCI Insight

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Section: Discussionsupporting

confidence: 84%

Immune cell landscaping reveals a protective role for regulatory T cells during kidney injury and fibrosis

Durães¹,

Lafont²,

Beibel³

et al. 2020

JCI Insight

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“…These phenomena may be crucial in diseases attributable to the insufficient clearance of immune complexes 24 . The proangiogenic activity of kidney-resident macrophages during renal artery stenosis was also reported 25 . This frontline and potential homeostatic activity of rMac may be related to their high proportion in mouse kidneys.…”

Section: Discussionmentioning

confidence: 89%

Immune cell composition in normal human kidneys

Park

Kim

et al. 2020

Sci Rep

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An understanding of immunological mechanisms in kidney diseases has advanced using mouse kidneys. However, the profiling of immune cell subsets in human kidneys remains undetermined, particularly compared with mouse kidneys. Normal human kidneys were obtained from radically nephrectomised patients with urogenital malignancy (n = 15). Subsequently, human kidney immune cell subsets were analysed using multicolor flow cytometry and compared with subsets from C57BL/6 or BALB/c mice under specific pathogen-free conditions. Twenty kidney sections from healthy kidney donors or subjects without specific renal lesions were additionally analysed by immunohistochemistry. In human kidneys, 47% ± 12% (maximum 63%) of immune cells were CD3+ T cells. Kidney CD4+ and CD8+ T cells comprised 44% and 56% of total T cells. Of these, 47% ± 15% of T cells displayed an effector memory phenotype (CCR7− CD45RA− CD69−), and 48% ± 19% were kidney-resident cells (CCR7− CD45RA− CD69+). However, the proportions of human CD14+ and CD16+ myeloid cells were approximately 10% of total immune cells. A predominance of CD3+ T cells and a low proportion of CD14+ or CD68+ myeloid cells were also identified in healthy human kidney sections. In mouse kidneys, kidney-resident macrophages (CD11blow F4/80high) were the most predominant subset (up to 50%) but the proportion of CD3+ T cells was less than 20%. These results will be of use in studies in which mouse results are translated into human cases under homeostatic conditions or with disease.

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“…PRJNA478258 [54] Kidney-resident and monocyte-derived subpopulations, effect of ischaemia (F4/80, CD64, CD11B, CD11C, MHCII).…”

Section: Gse116094mentioning

confidence: 99%

Network analysis of transcriptomic diversity amongst resident tissue macrophages and dendritic cells in the mouse mononuclear phagocyte system

2020

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The mononuclear phagocyte system (MPS) is a family of cells including progenitors, circulating blood monocytes, resident tissue macrophages, and dendritic cells (DCs) present in every tissue in the body. To test the relationships between markers and transcriptomic diversity in the MPS, we collected from National Center for Biotechnology Information Gene Expression Omnibus (NCBI-GEO) a total of 466 quality RNA sequencing (RNA-seq) data sets generated from mouse MPS cells isolated from bone marrow, blood, and multiple tissues. The primary data were randomly downsized to a depth of 10 million reads and requantified. The resulting data set was clustered using the network analysis tool BioLayout. A sample-to-sample matrix revealed that MPS populations could be separated based upon tissue of origin. Cells identified as classical DC subsets, cDC1s and cDC2s, and lacking Fcgr1 (encoding the protein CD64) were contained within the MPS cluster, no more distinct than other MPS cells. A gene-to-gene correlation matrix identified large generic coexpression clusters associated with MPS maturation and innate immune function. Smaller coexpression gene clusters, including the transcription factors that drive them, showed higher expression within defined isolated cells, including monocytes, macrophages, and DCs isolated from specific tissues. They include a cluster containing Lyve1 that implies a function in endothelial cell (EC) homeostasis, a cluster of transcripts enriched in intestinal macrophages, and a generic lymphoid tissue cDC cluster associated with Ccr7. However, transcripts encoding Adgre1, Itgax, Itgam, Clec9a, Cd163, Mertk, Mrc1, Retnla, and H2-a/e (encoding class II major histocompatibility complex [MHC] proteins) and many other proposed macrophage subset and DC lineage markers each had idiosyncratic expression profiles. Coexpression of immediate early genes (for example, Egr1, Fos, Dusp1) and inflammatory cytokines and chemokines (tumour necrosis factor [Tnf], Il1b, Ccl3/4) indicated that all tissue disaggregation and separation protocols activate MPS cells. Tissue-specific expression clusters indicated that all cell isolation procedures also co-purify other unrelated cell types that may interact with MPS cells in vivo. Comparative analysis of RNA-seq and single-cell RNA-seq (scRNA-seq) data from the same lung cell populations indicated that MPS

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Kidney-resident macrophages promote a proangiogenic environment in the normal and chronically ischemic mouse kidney

Cited by 84 publications

References 66 publications

Immune cell landscaping reveals a protective role for regulatory T cells during kidney injury and fibrosis

Immune cell landscaping reveals a protective role for regulatory T cells during kidney injury and fibrosis

Immune cell composition in normal human kidneys

Network analysis of transcriptomic diversity amongst resident tissue macrophages and dendritic cells in the mouse mononuclear phagocyte system

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