Kidney Mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats

LIANG, RUI-NING; Yan, Di; Zhang, Xingping; Chen, Xu; ZHANG, WEN-HUI; JIA, HONG-LIN

doi:10.1016/j.tice.2022.101998

Cited by 5 publications

(2 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the DNA synthesis phase (S phase), BrdU can replace thymine and infiltrate into the replicated DNA molecule, and then stained with fluorescent labeled BrdU antibody, thus the cell proliferation can be detected [ 38 ]. Using BrdU method to investigate the effects of syr+cos on the proliferation of L-02 cells.…”

Section: Resultsmentioning

confidence: 99%

Hepatoprotective effect of syringin combined with costunolide against LPS-induced acute liver injury in L-02 cells via Rac1/AKT/NF-κB signaling pathway

Mao,

Tan,

Tian

et al. 2023

Aging

View full text Add to dashboard Cite

Acute liver injury (ALI) leads to abnormal liver function and damage to liver cells. Syringin (syr) and costunolide (cos) are the major extracts from Dolomiaea souliei (Franch.) C.Shih ( D. souliei ), showing diverse biological functions in various biological processes. We explored the underlying hepatoprotective effects of syr+cos against LPS-induced ALI. Cell viability and proliferation were assessed using an MTT assay and immunofluorescence staining. Flow cytometry analysis was used to detect cell cycle distribution and apoptosis. ELISA was utilized to measure liver function and antioxidant stress indexes. qRT-PCR and western blotting was performed to determine mRNA and protein levels respectively. Using shRNA approach to Rac1 analyzed transcriptional targets. The results showed that syr+cos promoted L-02 cell proliferation, inhibiting the cell apoptosis and blocking cell cycle in G1 and G2/M phase. Syr+cos decreased the production of ALT, AST, LDH, MDA and ROS while increased SOD and CAT activities. Pretreated with syr+cos may decrease expressions of caspase-3,7,9, NF-κB, TNF-α proteins, Cyclin B, CDK1 and p-IκB proteins while p-IκB increased. Silencing of Rac-1 may protect the liver by increasing AKT, S473, T308 and reducing p-AKT proteins. Syr+cos exhibits anti-ALI activity via Rac1/AKT/NF-κB signaling pathway which might act as an effective candidate drug for the treatment of ALI.

show abstract

Section: Resultsmentioning

confidence: 99%

Hepatoprotective effect of syringin combined with costunolide against LPS-induced acute liver injury in L-02 cells via Rac1/AKT/NF-κB signaling pathway

Mao,

Tan,

Tian

et al. 2023

Aging

View full text Add to dashboard Cite

show abstract

“…Kidney sections were preheated, treated with silver staining fluid and washed with distilled water. Then, the sections were incubated in 2% gold chloride solution, washed, treated in 2% sodium thiosulfate solution, washed, double stained with hematoxylin or eosin, dehydrated in graded ethanol, cleared in xylene, and sealed with neutral gum [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Astragaloside IV relieves passive heymann nephritis and podocyte injury by suppressing the TRAF6/NF-κb axis

Ma,

Hu,

Ruan

et al. 2024

Renal Failure

View full text Add to dashboard Cite

The pathogenesis of membranous nephropathy (MN) involves podocyte injury that is attributed to inflammatory responses induced by local immune deposits. Astragaloside IV (AS-IV) is known for its robust anti-inflammatory properties. Here, we investigated the effects of AS-IV on passive Heymann nephritis (PHN) rats and TNF-α-induced podocytes to determine the underlying molecular mechanisms of MN. Serum biochemical parameters, 24-h urine protein excretion and renal histopathology were evaluated in PHN and control rats. The expression of tumor necrosis factor receptor associated factor 6 (TRAF6), the phosphorylation of nuclear factor kappa B (p-NF-κB), the expression of associated proinflammatory cytokines (TNF-α, IL-6 and IL-1β) and the ubiquitination of TRAF6 were measured in PHN rats and TNF-α-induced podocytes. We detected a marked increase in mRNA expression of TNF-α, IL-6 and IL-1β and in the protein abundance of p-NF-κB and TRAF6 within the renal tissues of PHN rats and TNF-α-induced podocytes. Conversely, there was a reduction in the K48-linked ubiquitination of TRAF6. Additionally, AS-IV was effective in ameliorating serum creatinine, proteinuria, and renal histopathology in PHN rats. This effect was concomitant with the suppression of NF-κB pathway activation and decreased expression of TNF-α, IL-6, IL-1β and TRAF6. AS-IV decreased TRAF6 levels by promoting K48-linked ubiquitin conjugation to TRAF6, which triggered ubiquitin-mediated degradation. In summary, AS-IV averted renal impairment in PHN rats and TNF-α-induced podocytes, likely by modulating the inflammatory response through the TRAF6/NF-κB axis. Targeting TRAF6 holds therapeutic promise for managing MN.

show abstract

Cardioprotective effect of oleuropein in a cisplatin-induced cardiotoxicity model in rats

Gökçek

2023

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

Kidney Mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats

Cited by 5 publications

References 57 publications

Hepatoprotective effect of syringin combined with costunolide against LPS-induced acute liver injury in L-02 cells via Rac1/AKT/NF-κB signaling pathway

Hepatoprotective effect of syringin combined with costunolide against LPS-induced acute liver injury in L-02 cells via Rac1/AKT/NF-κB signaling pathway

Astragaloside IV relieves passive heymann nephritis and podocyte injury by suppressing the TRAF6/NF-κb axis

Cardioprotective effect of oleuropein in a cisplatin-induced cardiotoxicity model in rats

Contact Info

Product

Resources

About