Kidney membrane cyclic AMP receptor and cyclic AMP-dependent protein kinase activities: Comparison of plasma membrane and cytoplasmic fractions

Cyclic AMP-dependent protein kinase activity and endogenous protein phosphorylation in isolated cortical segments of rabbit nephron.

“…These obser vations are consistent witi the reported protein kinase activity of the kidney cortex (15,17). The substrate specificity of the protein kinases of the 6 cortical nephron segments were nearly identical.…”

“…Cyclic AMP-dependent protein kinase and endogenous phosphorylation of the renal cortex have been studied in the cortical cytosol and membrane fractions (15)(16)(17), brush border membranes (18)(19)(20)(21), cortical slices and isolated tubules (22). However, in order to further elucidate the role of cyclic AMP-dependent protein kinase in the physiological function of the renal cortex, it is necessay to investigate the enzyme in the morphologically defined structures of the renal cortex.…”

Distribution of membrane-bound cyclic AMP-dependent protein kinase in plasma membranes of cells of the kidney cortex

“…The identification in renal tissue of cytosolic (Kuo & Greengard, 1969;Winickoff& Aurbach, 1970;Dousa, Sands & Hechter, 1972;Forte et al, 1972;Wombacher, Reuter-Smerdka & K6rber, 1973) and membranebound (Forte et al, 1972;Shlatz, Kinne, Kinne-Saffran & Schwartz, 1973) cyclic AMP-dependent protein kinases led to the idea that these enzymes may play an effector role in the regulation of tubular transport processes by hormones which act through the adenylate cyclase-cyclic AMP system. Such a role implies that some component of the affected transport system serves as a substrate for the protein kinase and that this substrate is more active at one pole of the transporting cell.…”

“…Since the effects of adenosine 31,51-monophosphate may be mediated through regulation of the activity of 146 R. Kinne et al cyclic AMP-dependent protein kinases, it is of interest to determine whether such an enzyme is a component of the proximal tubular epithelial plasma membrane and whether it is also localized at one pole of the cell. Recently, plasma membranes isolated from the hog kidney cortex (Forte, Chao, Walkenbach & Byington, 1972) were shown to contain a cyclic AMP-dependent protein kinase. The properties of this enzyme are similar to other intrinsic protein kinases found in brain, synaptosomal and microsomal membranes (Weller & Rodnight, 1970, 1971Ueda, Maeno & Greengard, 1973), adenohypophyseal secretory granules (Labrie, Lemaire, Poirier, Pelletier & Boucher, 1971;, anterior pituitary gland microsomes (Lemay, Deschenes, Lemaire, Poirier, Poulin & Labrie, 1974), cardiac sarcoplasmic reticulum (Wray, Gray & Olsson, 1973), and erythrocyte membranes (Guthrow, Allen & Rasmussen, 1972;Rubin, Erlichman & Rosen, 1972;Roses & Appel, 1973;Rubin & Rosen, 1973).…”