Kidney diseases and tissue engineering

Moon, Kee‐Chan; Ko, In Kap; Yoo, James J.; Atala, Anthony

doi:10.1016/j.ymeth.2015.06.020

Cited by 54 publications

(28 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tissue engineering applications include: bone (Bye et al 2013;Phipps et al 2012), cartilage (Steele et al 2014), ligament (Petrigliano et al 2014), cornea (Baradaran-Rafii et al 2016) and vascular (Wise et al 2011) to name a few. Polymer-based scaffolds, such as PCL, have been highlighted as a potential avenue for tissue engineered kidneys (Moon et al 2016), but there is little investigation down this stream.…”

Section: Introductionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

View full text Add to dashboard Cite

There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

show abstract

Section: Introductionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

View full text Add to dashboard Cite

show abstract

“…This process includes some growth factors that are provided in renal tissues. The growth factors include epidermal growth factor (EGF), a potent proximal tubule cell mitogen [188]; transforming growth factor-α (TGF-α), a participant in the reconstruction of the injured proximal tubule via the EGF receptor [188]; insulin-like growth factor 1 (IGF-1); fibroblast growth factor (FGF), and hepatocyte growth factor (HGF) [189]. Higuita-Castro et al have been successful in developing a platform that mimics the micro/nanoscale architecture of the alveolar environment.…”

Section: Hybrid Biomaterials Applied For Kidneysmentioning

confidence: 99%

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

2020

View full text Add to dashboard Cite

In this review, materials based on polymers and hybrids possessing both organic and inorganic contents for repairing or facilitating cell growth in tissue engineering are discussed. Pure polymer based biomaterials are predominantly used to target soft tissues. Stipulated by possibilities of tuning the composition and concentration of their inorganic content, hybrid materials allow to mimic properties of various types of harder tissues. That leads to the concept of "one-matches-all" referring to materials possessing the same polymeric base, but different inorganic content to enable tissue growth and repair, proliferation of cells, and the formation of the ECM (extra cellular matrix). Furthermore, adding drug delivery carriers to coatings and scaffolds designed with such materials brings additional functionality by encapsulating active molecules, antibacterial agents, and growth factors. We discuss here materials and methods of their assembly from a general perspective together with their applications in various tissue engineering sub-areas: interstitial, connective, vascular, nervous, visceral and musculoskeletal tissues. The overall aims of this review are two-fold: (a) to describe the needs and opportunities in the field of bio-medicine, which should be useful for material scientists, and (b) to present capabilities and resources available in the area of materials, which should be of interest for biologists and medical doctors.Polymers 2020, 12, 620 2 of 30 allows to tailor functionalize the coatings, where their responsiveness to external stimuli is one of their biggest advantages.Polymers stand out as a special class of materials due to the flexibility of design of their blocks and various functionalities, which is brought by their versatile assembly or by introducing additional side-groups or blocks, in the case of block-co-polymers. In this regard, both synthetic and biocompatible polymers are available, and polymer engineering represents a growing area tailoring the needs often driven by applications. With all advantages and the flexibility of the design, there is one significant weakness of polymers-the softness. In regard with tissue engineering, that translates to the fact that some materials can match predominantly soft tissue, but it is difficult to tune mechanical properties of polymers to match the hardness of bones, tendons, etc. And since mechanical properties of materials affect and even drive cell and tissue growth, enhancement of mechanical properties of polymers and biomaterials is essential. Chemical crosslinking can be applied to address these challenges, but that solves problems only partially.To solve these challenges the so-called hybrid materials [6] are used, which possess both inorganic and organic contents. Increasingly, hybrid materials are used in designing the extracellular matrix. The hybrid matrix design for various tissue engineering applications should meet bio-medical requirements. On the one hand, it needs to be biocompatible and biodegradable, which is achieved through the po...

show abstract

“…Regenerative medicine and tissue engineering are exploring treatment options ranging from cell therapy to creation of whole new kidneys for organ transplantation [72]. Stem cell sources from the kidney may prove ideal for these applications because of inherent renal differentiation potential and the potential for autologous treatments.…”

Section: Application Of Adult Renal Stem Cellsmentioning

confidence: 99%

Comparing adult renal stem cell identification, characterization and applications

Huling

Yoo

2017

J Biomed Sci

Self Cite

View full text Add to dashboard Cite

Despite growing interest and effort, a consensus has yet to be reached in regards to the identification of adult renal stem cells. Organ complexity and low turnover of renal cells has made stem cell identification difficult and lead to the investigation of multiple possible populations. In this review, we summarize the work that has been done toward finding and characterizing an adult renal stem cell population. In addition to giving a general overview of what has been done, we aim to highlight the variation in methods and outcomes. The methods used to locate potential stem cell populations can vary widely, but even within the relatively standard practice of BrdU labeling of slowly dividing cells, there are significant differences in protocols and results. Additional diversity exists in cell marker profiles and apparent differentiation potential seen in potential stem cell sources. Cataloging the variety of methods and outcomes seen so far may help to streamline future investigation and stear the field toward consensus. But even without firmly defined populations, the application of renal stem cells holds tantalizing potential. Populations of highly proliferative, multipotent cells of renal origin show the ability to engraft in injured kidneys, mitigate functional loss and occasionally show the ability to generate nephrons de novo. The progress toward regenerative medicine applications is also summarized.

show abstract

Kidney diseases and tissue engineering

Cited by 54 publications

References 97 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

Comparing adult renal stem cell identification, characterization and applications

Contact Info

Product

Resources

About