Kidney, blood, and endothelium: Developmental expression of stem cell leukemia during nephrogenesis

Dekel, Benjamin; Hochman, Eldar; Sánchez, María José; Maharshak, Nitsan; Amariglio, Ninette; Green, Anthony; Izraeli, Shai

doi:10.1111/j.1523-1755.2004.00489.x

Cited by 24 publications

(23 citation statements)

References 30 publications

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“…36 Given that SCL functions in hem-endothelial differentiation, it was surprising to find resident interstitial SCL 3 0 En-expressing cells in the adult kidney. 24 We now characterized these cells among which are hematopoietic (CD45 þ CD31À), mature endothelial (CD45ÀCD31 þ ), and surprisingly a relatively large fraction of nonendothelial non-hematopoietic 23 Clearly, the bone marrow contributed to formation of mature endothelial cells in peri-tubular capillaries of the kidney, representing postnatal vasculogenesis. Identification of donorderived endothelial cells in the liver suggesting that this phenomenon is widespread and not limited to the kidney is in agreement with previous reports.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, we could identify SCL 3 0 En-expressing cells in the adult renal interstitium. 24 The SCL 3 0 En driving b-galactosidase as a reporter gene is detected in hematopoietic cells and vasculature during development. However, a high degree of variation in reporter gene expression is observed in the vasculature of adult tissues, possibly due to the interaction of the regulatory elements of the 3 0 En and the lacZ reporter gene.…”

Section: Origin Of Scl 3 0 En-expressing Cells In the Adult Kidneymentioning

confidence: 99%

“…24 The early and specific expression of SCL 3 0 En in the metanephric mesenchyme suggests a role in the differentiation and growth of the mammalian kidney, especially in specifying angioblastic progenitors. Interestingly, although postnatal SCL message was abrogated, we observed SCL 3 0 En ( þ ) cells resident in the adult kidney.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, although postnatal SCL message was abrogated, we observed SCL 3 0 En ( þ ) cells resident in the adult kidney. 24 Renal developmental pathways have been shown to be activated during adult tissue regeneration, 25,26 providing possible links for both processes. In addition, blood and marrow-derived cells have been recently suggested to have vasculogenic potential during kidney damage.…”

Section: Introductionmentioning

confidence: 99%

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Organ-injury-induced reactivation of hemangioblastic precursor cells

et al. 2007

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Early in mammalian development, the stem cell leukemia (SCL/TAL1) gene and its distinct 3 0 enhancer (SCL 3 0 En) specify bipotential progenitor cells that give rise to blood and endothelium, thus termed hemangioblasts. We have previously detected a minor population of SCL ( þ ) cells in the postnatal kidney. Here, we demonstrate that cells expressing the SCL 3 0 En in the adult kidney are comprised of CD45 þ CD31À hematopoietic cells, CD45ÀCD31 þ endothelial cells and CD45ÀCD31À interstitial cells. Creation of bone marrow chimeras of SCL 3 0 En transgenic mice into wild-type hosts shows that all three types of SCL 3 0 En-expressing cells in the adult kidney can originate from the bone marrow. Ischemia/ reperfusion injury to the adult kidney of SCL 3 0 En transgenic mice results in the intrarenal elevation of SCL and FLK1 mRNA levels and of cells expressing hem-endothelial progenitor markers (CD45, CD34, c-Kit and FLK1). Furthermore, analysis of SCL 3 0 En in the ischemic kidneys reveals an increase in the abundance of SCL 3 0 En-expressing cells, predominantly within the CD45 ( þ ) hematopoietic fraction and to a lesser extent in the CD45 (À) fraction. Our results suggest organ-injury-induced reactivation of bone marrow-derived hemangioblasts and possible local angioblastic progenitors expressing SCL and SCL 3 0 En.

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Section: Discussionmentioning

confidence: 99%

Section: Origin Of Scl 3 0 En-expressing Cells In the Adult Kidneymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Organ-injury-induced reactivation of hemangioblastic precursor cells

et al. 2007

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“…A 6-hour, 60°C hybridization was performed, followed by three washes in 0.1ϫ SSC (1ϫ SSC is 0.15 M NaCl plus 0.015 M sodium citrate) at 75°C. Immunohistochemical staining for SCL was performed on mouse sections with a rabbit polyclonal anti-mouse SCL antibody (24) and on human bone marrow trephine sections with the anti-human SCL antibody 2TL242 (39) following protocols detailed elsewhere (8,24). Immunohistochemical staining of hPLAP was performed with a rabbit anti-human PLAP primary antibody (Serotec, AHP 537) followed by a biotinylated sheep anti-rabbit secondary antibody (Serotec, 2AB02B), detected with horseradish peroxidaseextravidin (Sigma) and visualized with 3-amino-9-ethylcarbazole according to the manufacturer (Serotec, BUF019A).…”

Section: Methodsmentioning

confidence: 99%

Transcriptional Link between Blood and Bone: the Stem Cell Leukemia Gene and Its +19 Stem Cell Enhancer Are Active in Bone Cells

Pimanda

Silberstein

Dominici

et al. 2006

Molecular and Cellular Biology

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Blood and vascular cells are generated during early embryogenesis from a common precursor, the hemangioblast. The stem cell leukemia gene (SCL/tal 1) encodes a basic helix-loop-helix transcription factor that is essential for the normal development of blood progenitors and blood vessels. We have previously characterized a panel of SCL enhancers including the ؉19 element, which directs expression to hematopoietic stem cells and endothelium. Here we demonstrate that SCL is expressed in bone primordia during embryonic development and in adult osteoblasts. Despite consistent expression in cells of the osteogenic lineage, SCL protein is not required for bone specification of embryonic stem cells. In transgenic mice, the SCL ؉19 core enhancer directed reporter gene expression to vascular smooth muscle and bone in addition to blood and endothelium. A 644-bp fragment containing the SCL ؉19 core enhancer was active in both blood and bone cell lines and was bound in vivo by a common array of Ets and GATA transcription factors. Taken together with the recent observation that a common progenitor can give rise to blood and bone cells, our results suggest that the SCL ؉19 enhancer targets a mesodermal progenitor capable of generating hematopoietic, vascular, and osteoblastic progeny.

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