Kibdelins, novel glycopeptide antibiotics. I Discovery, production, and biological evaluation.

A novel actinomycete, designated strain KLBMP 1111(T), was isolated from the root of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Strain KLBMP 1111(T) formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus Kibdelosporangium, with the highest similarity to Kibdelosporangium aridum subsp. aridum DSM 43828(T) (98.8%), K. aridum subsp. largum DSM 44150(T) (98.1%) and Kibdelosporangium philippinense DSM 44226(T) (98.1%). The organism produced sporangium-like structures, the typical morphological characteristic of the genus Kibdelosporangium. The chemotaxonomic properties of this strain were also consistent with those of the genus Kibdelosporangium: the peptidoglycan contained meso-diaminopimelic acid; the predominant menaquinone was MK-9(H(4)); phospholipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol and an unknown phospholipid; iso-C(16:0), C(16:0), anteiso-C(15:0) and iso-C(15:0) as the predominant cellular fatty acids and the G+C content was 67.2 mol%. DNA-DNA hybridization values between strain KLBMP 1111(T) and the three Kibdelosporangium species were less than 50%. This strain had the ability to produce a siderophore, utilized 1-aminocyclopropane-1-carboxylic acid (ACC) as sole source of nitrogen and possessed ACC deaminase enzyme. Based on genotypic and phenotypic data, strain KLBMP 1111(T) represents a novel species in the genus Kibdelosporangium. We propose the name Kibdelosporangium phytohabitans sp. nov. for this species. The type strain is the strain KLBMP 1111(T) (=KCTC 19775(T) = CCTCC AA 2010001(T)).

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“…aridum DSM 43828 T , K. aridum subsp. largum DSM 44150 T (Shearer et al 1986b) and K. philippinense DSM 44226 T (Mertz and Yao 1988).…”

Section: Introductionmentioning

confidence: 99%

Kibdelosporangium phytohabitans sp. nov., a novel endophytic actinomycete isolated from oil-seed plant Jatropha curcas L. containing 1-aminocyclopropane-1-carboxylic acid deaminase

Xing

Bian

Qin

et al. 2011

Antonie van Leeuwenhoek

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“…[93] The yields of ardacins and of the related kibdelins, produced in fermentations of Kibdelosporangium species, are significantly higher in the presence of methyl oleate, a fact attributed to the oleates facile conversion to acetyl-CoA, a precursor of both mdihydroxyphenylglycine and of the fatty acid groups of ardacins and kibdelins. [94] Soy meal, peptone, or yeast extract are frequently employed as nitrogen sources in the fermentation. Finally, several reports point to feedback inhibitions in the biosynthetic pathway.…”

Section: Structure Classification and Occurrence Of The Glycopeptidmentioning

confidence: 99%

Chemistry, Biology, and Medicine of the Glycopeptide Antibiotics

Nicolaou

Boddy

Bräse³

et al. 1999

Angew. Chem. Int. Ed.

719

445

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The war against infectious bacteria is not over! Although vancomycin and glycopeptide antibiotics have provided a strong last line of defence against many drug-resistant bacteria, their overuse has given rise to more dangerous strains of bacteria. An understanding of the chemistry and biology of these highly complex glycopeptides are destined to play a crucial role in the discovery of new antibiotics.

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“…[92] Alle Glycopeptid-Antibiotika, die bis heute isoliert worden sind, haben ihren Ursprung in der Ordnung Actinomycetales. [93] Die Ausbeuten an Ardacin und den verwandten Kibdelinen, die bei der Fermentierung von Kibdelosporangium-Arten erhalten werden, sind in Gegenwart von Methyloleat deutlich höher, was auf die leichte Umwandlung von Oleat zu Acetyl-CoA, einer Vorstufe sowohl von mDihydroxyphenylglycin als auch der Fettsäuregruppe in Ardacinen und Kibdelinen, [94] zurückzuführen ist. Als Stickstoffquellen werden häufig Sojamehl, Pepton oder Hefeextrakt bei der Fermentierung hinzugesetzt.…”

Section: Struktur Klassifizierung Und Vorkommen Der Glycopeptid-antiunclassified

Chemie, Biologie und medizinische Anwendungen der Glycopeptid-Antibiotika

Nicolaou

Boddy

Bräse³

et al. 1999

Angewandte Chemie

166

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Der Krieg gegen pathogene Bakterien ist noch nicht vorbei! Zwar bilden Vancomycin und andere Glycopeptid‐Antibiotika eine letzte Verteidigungslinie gegen zahlreiche mehrfachresistente Bakterien, doch ihr leichtfertiger Gebrauch hat zur Bildung etlicher gefährlicherer Bakterienstämme geführt. Das Verständnis der chemischen und biologischen Eigenschaften dieser hochkomplexen Glycopeptide dürfte eine entscheidende Rolle für die Entdeckung und Entwicklung neuer Antibiotika spielen.

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Kibdelins, novel glycopeptide antibiotics. I Discovery, production, and biological evaluation.

Cited by 38 publications

References 13 publications

Kibdelosporangium phytohabitans sp. nov., a novel endophytic actinomycete isolated from oil-seed plant Jatropha curcas L. containing 1-aminocyclopropane-1-carboxylic acid deaminase

Kibdelosporangium phytohabitans sp. nov., a novel endophytic actinomycete isolated from oil-seed plant Jatropha curcas L. containing 1-aminocyclopropane-1-carboxylic acid deaminase

Chemistry, Biology, and Medicine of the Glycopeptide Antibiotics

Chemie, Biologie und medizinische Anwendungen der Glycopeptid-Antibiotika

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